Abstract
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous compounds with carcinogenic and/or mutagenic potential. To address the limitations of individual remediation techniques and to achieve better PAH removal efficiencies, the combination of chemical and biological treatments can be used. The degradation of phenanthrene (chosen as a model of PAH) by persulfate in freshly contaminated soil microcosms was studied to assess its impact on the biodegradation process and on soil properties. Soil microcosms contaminated with 140 mg/kgDRY SOIL of phenanthrene were treated with different persulfate (PS) concentrations 0.86–41.7 g/kgDRY SOIL and incubated for 28 days. Analyses of phenanthrene and persulfate concentrations and soil pH were performed. Cultivable heterotrophic bacterial count was carried out after 28 days of treatment. Genetic diversity analysis of the soil microcosm bacterial community was performed by PCR amplification of bacterial 16S rDNA fragments followed by denaturing gradient gel electrophoresis (DGGE). The addition of PS in low concentrations could be an interesting biostimulatory strategy that managed to shorten the lag phase of the phenanthrene biological elimination, without negative effects on the physicochemical and biological soil properties, improving the remediation treatment.
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Acknowledgments
This work was partially supported by the Agencia Nacional de Promoción Científica y Tecnológica (PICT 2010-0366). Mora V. C. and Rosso J. A. are research members of CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas., Argentina), and Morelli I. S. is research member of CIC-PBA (Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Argentina). Madueño L. and Peluffo M. are doctoral fellows of CONICET.
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Mora, V.C., Madueño, L., Peluffo, M. et al. Remediation of phenanthrene-contaminated soil by simultaneous persulfate chemical oxidation and biodegradation processes. Environ Sci Pollut Res 21, 7548–7556 (2014). https://doi.org/10.1007/s11356-014-2687-0
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DOI: https://doi.org/10.1007/s11356-014-2687-0