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High throughput and miniaturised systems for biodegradability assessments

Environmental Science and Pollution Research volume 21, pages 9545–9552 (2014)Cite this article

Abstract

The society demands safer products with a better ecological profile. Regulatory criteria have been developed to prevent risks for human health and the environment, for example, within the framework of the European regulation REACH (Regulation (EC) No 1907, 2006). This has driven industry to consider the development of high throughput screening methodologies for assessing chemical biodegradability. These new screening methodologies must be scalable for miniaturisation, reproducible and as reliable as existing procedures for enhanced biodegradability assessment. Here, we evaluate two alternative systems that can be scaled for high throughput screening and conveniently miniaturised to limit costs in comparison with traditional testing. These systems are based on two dyes as follows: an invasive fluorescent dyes that serves as a cellular activity marker (a resazurin-like dye reagent) and a noninvasive fluorescent oxygen optosensor dye (an optical sensor). The advantages and limitations of these platforms for biodegradability assessment are presented. Our results confirm the feasibility of these systems for evaluating and screening chemicals for ready biodegradability. The optosensor is a miniaturised version of a component already used in traditional ready biodegradability testing, whereas the resazurin dye offers an interesting new screening mechanism for chemical concentrations greater than 10 mg/l that are not amenable to traditional closed bottle tests. The use of these approaches allows generalisation of high throughput screening methodologies to meet the need of developing new compounds with a favourable ecological profile and also assessment for regulatory purpose.

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References

  • AFNOR (1998) Qualité de l’eau—Détermination de la demande biochimique en oxygène après n jours (DBOn)—Partie 1: Méthode par dilution et ensemencement avec apport d’allyl thio-urée (ISO 5818: 1989, modifiée). EN 1899–1 pp 28

  • Akcha F, Spagnol C, Rouxel J (2012) Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos. Aquat Toxicol 106–107:104–113

    Article  Google Scholar 

  • Comber M, Holt M (2010) Developing a set of reference chemicals for use in biodegradability tests for assessing the persistency of chemicals. MCC Report: MCC/007: available at: LRi http:www.cefic-lri.org/

  • Cregut M, Bedas M, Assaf A, Durand-Thouand M-J, Thouand G (2013a) Applying Raman spectroscopy to the assessment of the biodegradation of industrial polyurethanes wastes. Environ Sci Pollut Res. doi:10.1007/s11356-013-1772-0

    Google Scholar 

  • Cregut M, Piutti S, Slezack-Deschaumes S, Benizri E (2013b) Compartmentalization and regulation of arylsulfatase activities in Streptomyces sp., Microbacterium sp. and Rhodococcus sp. soil isolates in response to inorganic sulfate limitation. Microbiol Res 168:12–21

    CAS  Article  Google Scholar 

  • Deshpande RR, Wittmann C, Heinzle E (2004) Microplates with integrated oxygen sensing for medium optimization in animal cell culture. Cytotechnology 46:1–8

    CAS  Article  Google Scholar 

  • European Commission- European Chemicals Bureau (2000a) IUCLID Dataset: Substance ID: 532-32-1. http://esis.jrc.ec.europa.eu/doc/IUCLID/data_sheets/532321.pdf Accessed 5 Jun 2013

  • Haltrich (1983) GSF-Bericht Oe-714 cited in European Commission- European Chemicals Bureau (2000b) IUCLID Dataset: Substance ID: 100-02-7. http://esis.jrc.ec.europa.eu/doc/IUCLID/data_sheets/100027.pdf Accessed 5 Jun 2013

  • Gerike P, Fischer WK (1979) A correlation study of biodegradability determinations with various chemicals in various tests. Ecotoxicol Environ Saf 3:159–173

    CAS  Article  Google Scholar 

  • Guyard C (2010) BOD5: a rising parameter. L’eau, l’industrie, les nuisances. (ISSN: 0755–5016) 334:51–58

  • Hancock MK, Hermanson SB, Dolman NJ (2012) A quantitative TR-FRET plate reader immunoassay for measuring autophagy. Autophagy 8(8):1227–1244

    CAS  Article  Google Scholar 

  • Ingerslev F, Torang L, Nyholm N (2000) Importance of the test volume on the lag phase in biodegradation studies. Environ Toxicol Chem 19:2443–2447

    CAS  Article  Google Scholar 

  • Kensy F, John GT, Hofmann B, Büchs J (2005) Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates. Bioprocess Biosyst Eng 75:75–81

    Article  Google Scholar 

  • King EF, Painter HA (1983) Ring-test programme 1981–82. Assessment of biodegradability of chemicals in water by manometric respirometry, Commission of the European Communities, Luxembourg, Contract No. W/81/217

  • Köster M, Paffenhöfer G-A, Baker CV, Williams JE (2010) Oxygen consumption of doliolids (Tunicata thaliacea). J Plankton Res 32(2):171–180

    Article  Google Scholar 

  • Nyholm N, Lindgaard-Jorgensen P, Hansen N (1984) Biodegradation of 4-nitrophenol in standardised aquatic degradation tests. Ecotoxiol Environ Saf 8:451–470

    CAS  Article  Google Scholar 

  • O’Brien J, Wilson I, Orton T, Pognan F (2000) Investigation of the Alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 267:5421–5426

    Article  Google Scholar 

  • OECD (1992a). Ready biodegradability. 301 B CO2 evolution (modified Sturm test). OECD Guideline for Testing of Chemicals. Organisation for Economic Cooperation and Development: Paris; p 18–24

  • OECD (1992b). Ready biodegradability. 301 D Closed bottle test. OECD Guideline for Testing of Chemicals. Organisation for Economic Cooperation and Development: Paris; p 32–40

  • OECD (1992c). Ready biodegradability. 301 F Manometric respirometry test. OECD Guideline for Testing of Chemicals. Organisation for Economic Cooperation and Development: Paris; p 48–55

  • Potapova I, Eglin D, Laschke MW, Bischoff M, Geoff Richards R, Fintan Moriarty T (2013) Two-step labelling of Staphylococcus aureus with Lysostaphin-Azide and DIBO-Alexa using chemistry. J Microbiol Meth 92(1):90–98

    CAS  Article  Google Scholar 

  • Regulation (EC) No 1907 (2006) of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH), establishing a European Chemicals Agency, amending Directive 1999/45/EC and repealing Council Regulation (EEC) No 793/93 and Commission Regulation (EC) No 1488/94 as well as Council Directive 76/769/EEC and Commission Directives 91/155/EEC, 93/67/EEC, 93/105/EC and 2000/21/EC

  • Richterich K, Steber J (1989) Prevention of nitrification-caused erroneous biodegradability data in the closed bottle test. Chemosphere 19(10–11):1643–1654

    CAS  Article  Google Scholar 

  • Royal Commission on Sewage Disposal (1908) Fifth report: treating and disposing of sewage. Br Med J 7:1447

    Google Scholar 

  • Salanitro JP, Langston GC, Dorn PB, Kravetz L (1988) Activated sludge treatment of ethoxylate surfactants at high industrial use concentrations. Water Sci Technol 20(11–12):125–130

    CAS  Google Scholar 

  • Strecker R, Seiler T-B, Hollert H, Braunbeck T (2011) Oxygen requirements of zebrafish (Danio rerio) embryos in embryo toxicity tests with environmental samples. Comp Biochem Physiol C Toxicol Pharmacol 153:318–327

    Article  Google Scholar 

  • Sykes ML, Baell JB, Kaiser M, Chatelain E, Moawad SR, Ganame D, Ioset J-R, Avery VM (2011) Identification of compounds with anti-proliferative activity against Trypanosoma brucei brucei strain 427 by a whole cell viability based HTS campaign. PLoS Negl Trop Dis 6(11):e1896

    Article  Google Scholar 

  • Thouand G, Capdeville B, Block JC (1996) Pre-adapted inocula for limiting the risk of errors in biodegradability tests. Ecotoxicol Environ Saf 33:261–267

    CAS  Article  Google Scholar 

  • Thouand G, Durand MJ, Maul A, Gancet C, Blok H (2011) New concepts in the evaluation of biodegradation/persistence of chemical substances using a microbial inoculums. Front Microbiol 2:164

    Article  Google Scholar 

  • Thouand G, Friant G, Friant P, Bois F, Cartier A, Maul A, Block JC (1995) Bacterial inoculums density and probability of para-nitrophenol biodegradability test response. Ecotoxicol Environ Saf 30:274–282

    CAS  Article  Google Scholar 

  • Wesolowski J, Hassan RYA, Hodde S, Bardroff C, Bilitewski U (2008) Sensing of oxygen in microtiter plates: a novel tool for screening drugs against pathogenic yeasts. Anal Bioanal Chem 391:1731–1737

    CAS  Article  Google Scholar 

  • Yashiki Y, Yamashoji S (1996) Extracellular reduction of menadione and ferricyanide in yeast cell suspension. J Ferment Bioeng 82:319–321

    CAS  Article  Google Scholar 

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Authors and Affiliations

  1. UMR CNRS 6144 GEPEA CBAC lab, University of Nantes, 18 Blvd Gaston Defferre, 85035, La Roche sur Yon, France

    Mickael Cregut, Sulivan Jouanneau, François Brillet, Marie-José Durand, Cyril Sweetlove & Gérald Thouand

  2. L’Oréal, 1 Ave Eugène Schueller, 93600, Aulnay-sous-Bois, France

    Cyril Sweetlove, Jean-Charles Chenèble & Jacques L’Haridon

Authors
  1. Mickael Cregut
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  2. Sulivan Jouanneau
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  3. François Brillet
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  4. Marie-José Durand
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  5. Cyril Sweetlove
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  6. Jean-Charles Chenèble
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  7. Jacques L’Haridon
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  8. Gérald Thouand
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Correspondence to Gérald Thouand.

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Responsible editor: Philippe Garrigues

This short communication is submitted for publication in the special issue dedicated to biodegradation.

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Cregut, M., Jouanneau, S., Brillet, F. et al. High throughput and miniaturised systems for biodegradability assessments. Environ Sci Pollut Res 21, 9545–9552 (2014). https://doi.org/10.1007/s11356-013-2236-2

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  • DOI: https://doi.org/10.1007/s11356-013-2236-2

Keywords

  • Biodegradation
  • Ready biodegradability test
  • Biodegradability assessment
  • Optical sensor
  • Resazurin dye reagent