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Enhanced reduction of phenol content and toxicity in olive mill wastewaters by a newly isolated strain of Coriolopsis gallica

Environmental Science and Pollution Research volume 21, pages 1746–1758 (2014)Cite this article

Abstract

The search for novel microorganisms able to degrade olive mill wastewaters (OMW) and withstand the toxic effects of the initially high phenolic concentrations is of great scientific and industrial interest. In this work, the possibility of reducing the phenolic content of OMW using new isolates of fungal strains (Coriolopsis gallica, Bjerkandera adusta, Trametes versicolor, Trichoderma citrinoviride, Phanerochaete chrysosporium, Gloeophyllum trabeum, Trametes trogii, and Fusarium solani) was investigated. In vitro, all fungal isolates tested caused an outstanding decolorization of OMW. However, C. gallica gave the highest decolorization and dephenolization rates at 30 % v/v OMW dilution in water. Fungal growth in OMW medium was affected by several parameters including phenolic compound concentration, nitrogen source, and inoculum size. The optimal OMW medium for the removal of phenolics and color was with the OMW concentration (in percent)/[(NH4)2SO4]/inoculum ratio of 30:6:3. Under these conditions, 90 and 85 % of the initial phenolic compounds and color were removed, respectively. High-pressure liquid chromatography analysis of extracts from treated and untreated OMW showed a clear and substantial reduction in phenolic compound concentrations. Phytotoxicity, assessed using radish (Raphanus sativus) seeds, indicated an increase in germination index of 23–92 % when a 30 % OMW concentration was treated with C. gallica in different dilutions (1/2, 1/4, and 1/8).

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Affiliations

  1. Laboratory of Enzyme Engineering and Microbiology, Ecole Nationale d’Ingénieurs de Sfax, University of Sfax, Route de Soukra km 4.5, BP 1173, 3038, Sfax, Tunisia

    Dalel Daâssi, Moncef Nasri & Tahar Mechichi

  2. Laboratory of Soil Biology, University of Neuchatel, Rue Emile Argand 11, 2009, Neuchatel, Switzerland

    Lassaad Belbahri

  3. Institute of Chemistry, University of Neuchatel, Avenue de Bellevaux 51, 2000, Neuchatel, Switzerland

    Armelle Vallat

  4. Department of Plant and Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen, AB24 3UU, Scotland, UK

    Steve Woodward

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  1. Dalel Daâssi
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  2. Lassaad Belbahri
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  3. Armelle Vallat
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  4. Steve Woodward
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  5. Moncef Nasri
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  6. Tahar Mechichi
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Correspondence to Tahar Mechichi.

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Daâssi, D., Belbahri, L., Vallat, A. et al. Enhanced reduction of phenol content and toxicity in olive mill wastewaters by a newly isolated strain of Coriolopsis gallica . Environ Sci Pollut Res 21, 1746–1758 (2014). https://doi.org/10.1007/s11356-013-2019-9

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Keywords

  • Laccase
  • Coriolopsis gallica
  • Oil mill wastewater
  • Phenolic compounds