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Environmental Science and Pollution Research

, Volume 20, Issue 2, pp 676–689 | Cite as

Responses of juvenile European flounder (Platichthys flesus) to multistress in the Vilaine estuary, during a 6-month survey

  • Estérine Evrard
  • Alain Devaux
  • Sylvie Bony
  • Jérôme Cachot
  • Grégory Charrier
  • Louis Quiniou
  • Jean LarocheEmail author
Ecotoxicology of estuaries in France and Québec, Canada

Abstract

Physiological and genetic responses of age 0+ Platichthys flesus were investigated in the eutrophicated and moderately contaminated Vilaine estuary, during a 6-month survey. The main objective of this study was to explore the biological responses of fishes during their juvenile period in an estuarine system in order to detect a possible selective pressure induced by the environmental stress. Our results showed a general convergence in physiological responses along the survey: an increase in genotoxicity was associated with an increase in mRNA expression of ATPase and betaine homocysteine methyltransferase. These results could suggest an increase of cellular damage, energetic request, and detoxification rate related to the growing exposure time to stress. Considering the aging of the cohort, the genetic characteristics of the Vilaine flounder cohort came closer to the one observed in a highly stressed system, the Seine estuary, suggesting a potential selective pressure mainly induced by the chemical stress.

Keywords

Flounder Chemical contamination DNA damage Genetic variability 

Notes

Acknowledgments

This study was supported by the INTERREG program: DIESE by the DEVIL-INERIS program and by the EVOLFISH project (ANR-VMCS). Authors also thank F. and R. Triballier (Vilaine) and C. Drouin (Seine) for field sampling. The authors also wish to thank Dr Henrique Cabral for his helpful comments on the manuscript.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Estérine Evrard
    • 1
  • Alain Devaux
    • 2
  • Sylvie Bony
    • 2
  • Jérôme Cachot
    • 3
  • Grégory Charrier
    • 4
  • Louis Quiniou
    • 5
  • Jean Laroche
    • 5
    Email author
  1. 1.CEDRE, rue Alain ColasBrest Cedex 2France
  2. 2.USC INRA “Impact génotoxique sur les hydrosystèmes”, UMR LEHNA 5023, ENTPE, Rue Maurice AudinCedexFrance
  3. 3.Univ. Bordeaux, EPOC UMR 5805, LPTC group, avenue des FacultésTalenceFrance
  4. 4.Department of Marine EcologyUniv. GothenburgStrömstadSweden
  5. 5.Université Européenne de Bretagne, Université de Brest, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l’Environnement Marin LEMARInstitut Universitaire Européen de la MerPlouzanéFrance

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