Abstract
A new polyclonal antibody (pAb) was prepared and used for the determination of polychlorinated biphenyls (PCBs) in air samples to promote the application of immunoassay technology in the determination of PCBs. Three PCB congeners immunogen mixture was used to stimulate immune responses in rabbits. The specific pAb to PCBs was obtained and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). A standard curve for Aroclor 1248 was prepared using concentrations ranging from 0.1 to 100 μg L−1. The average IC50 value was 16.21 μg L−1 and the limit of detection at 10 % inhibition (IC90) was 0.069 μg L−1. The entire procedure was then evaluated using spiked air samples. The recoveries of Aroclor 1248 at various spiking levels in the air samples ranged from 84 to 113 %, with relative standard deviations of 3 to 6 %. Under optimum conditions, the cross-reactivity profiles of the assays were obtained using three selected congeners, four Aroclor products, and other structurally related compounds of PCBs. The assays were found to be highly specific for PCB congeners and Aroclors 1248 and 1242. The air samples were then analyzed using gas chromatography coupled with high-resolution mass spectrometry to confirm the ic-ELISA results. The attained results demonstrated that the proposed method was an effective and inexpensive technique for the PCBs determination in air samples.
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Acknowledgments
The authors gratefully acknowledge the National Natural Science Foundation of China (Project No. 21177082), the Science and Technology Commission of Shanghai Municipality in China (Key Project of Fundamental Research No. 09JC1407600), and the Scientific and Technical Innovation Develop Special Founds of Shanghai Jiaotong University, and Fok Ying Tung Education Foundation for Excellent Youth Scholars (114044), as well as the Postdoctoral Science Foundation of Shanghai (No. 11R21414600) for their financial support.
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Chen, HY., Zhuang, HS., Yang, GX. et al. Development of a new polyclonal antibody for the determination of polychlorinated biphenyls in indoor air by ic-ELISA. Environ Sci Pollut Res 20, 2244–2251 (2013). https://doi.org/10.1007/s11356-012-1090-y
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DOI: https://doi.org/10.1007/s11356-012-1090-y