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Solid-state fermentation: tool for bioremediation of adsorbed textile dyestuff on distillery industry waste-yeast biomass using isolated Bacillus cereus strain EBT1

Abstract

Bioremediation of textile dyestuffs under solid-state fermentation (SSF) using industrial wastes as substrate pose an economically feasible, promising, and eco-friendly alternative. The purpose of this study was to adsorb Red M5B dye, a sample of dyes mixture and a real textile effluent on distillery industry waste-yeast biomass (DIW-YB) and its further bioremediation using Bacillus cereus EBT1 under SSF. Textile dyestuffs were allowed to adsorb on DIW-YB. DIW-YB adsorbed dyestuffs were decolorized under SSF by using B. cereus. Enzyme analysis was carried out to ensure decolorization of Red M5B. Metabolites after dye degradation were analyzed using UV–Vis spectroscopy, FTIR, HPLC, and GC-MS. DIW-YB showed adsorption of Red M5B, dyes mixture and a textile wastewater sample up to 87, 70, and 81 %, respectively. DIW-YB adsorbed Red M5B was decolorized up to 98 % by B. cereus in 36 h. Whereas B. cereus could effectively reduce American Dye Manufacture Institute value from DIW-YB adsorbed mixture of textile dyes and textile wastewater up to 70 and 100 %, respectively. Induction of extracellular enzymes such as laccase and azoreductase suggests their involvement in dye degradation. Repeated utilization of DIW-YB showed consistent adsorption and ADMI removal from textile wastewater up to seven cycles. HPLC and FTIR analysis confirms the biodegradation of Red M5B. GC-MS analysis revealed the formation of new metabolites. B. cereus has potential to bioremediate adsorbed textile dyestuffs on DIW-YB. B. cereus along with DIW-YB showed enhanced decolorization performance in tray bioreactor which suggests its potential for large-scale treatment procedures.

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Acknowledgments

The author Avinash A. Kadam is thankful to Department of Science and Technology, New Delhi for providing the DST-PURSE fellowship.

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Correspondence to Sanjay P. Govindwar.

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Responsible editor: Vinod Kumar Gupta

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Kadam, A.A., Kamatkar, J.D., Khandare, R.V. et al. Solid-state fermentation: tool for bioremediation of adsorbed textile dyestuff on distillery industry waste-yeast biomass using isolated Bacillus cereus strain EBT1. Environ Sci Pollut Res 20, 1009–1020 (2013). https://doi.org/10.1007/s11356-012-0929-6

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Keywords

  • Decolorization
  • Distillery industry waste-yeast biomass
  • Bacillus cereus
  • Red M5B
  • Solid-state fermentation