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Alteration of in vitro and acute in vivo toxicity of textile dyeing wastewater after chemical and biological remediation

Environmental Science and Pollution Research volume 19, pages 2634–2643 (2012)Cite this article

Abstract

Introduction

Textile industry is one of the most common and essential sectors in Tunisia. However, the treatment of textile effluents becomes a university because of their toxic impacts on waters, soils, flora, and fauna.

Materials and methods

The aim of this work was to evaluate the ability of Pseudomonas putida mt-2 to decolorize a textile wastewater and to compare the biologic decolorization process to the chemical one currently used by the textile industry.

Results

P. putida exhibited a high decolorizing capacity of the studied effluent, compared to the coagulation–flocculation method with decolorization percentage of 86% and 34.5%, respectively. Genotoxicity of the studied effluent, before and after decolorization by P. putida mt-2, was evaluated in vitro, using the SOS chromotest, and in vivo, in mouse bone marrow, by assessing the percentage of cells bearing different chromosome aberrations compared to not treated mice. In addition, textile effluent statistically significant influenced acetylcholinesterase and butyrylcholinesterase activities and lipid peroxidation (p < 0.01) when compared to not-treated mice. Coagulation–flocculation treatment process used by industry was revealed to be ineffective. Indeed toxicities persisted after treatment and the effluent did not show any statistically significant decrease in toxicities compared to non-treated effluent. Our results indicate that P. putida is a promising and improved alternative to treating industrial scale effluent compared to current chemical decolorization procedures used by the Tunisian textile industry.

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Affiliations

  1. Equipe de Recherche en Physico-chimie et Biotechnologie (E.R.P.C.B—EA3914), IUT-UFR Sciences, Université de Caen—Basse, Normandie, France

    Hedi Ben Mansour, Daniel Barillier & Ridha Mosrati

  2. Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médecine Dentaire, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisia

    Hedi Ben Mansour, Ikram Houas, Fadoua Montassar & Leila Chekir-Ghedira

  3. Unité de Recherche Pharmacognosie Biologie Moléculaire Faculté de Pharmacie, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisia

    Kamel Ghedira

Authors
  1. Hedi Ben Mansour
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  2. Ikram Houas
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  3. Fadoua Montassar
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  4. Kamel Ghedira
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  5. Daniel Barillier
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  6. Ridha Mosrati
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  7. Leila Chekir-Ghedira
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Corresponding author

Correspondence to Leila Chekir-Ghedira.

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Responsible editor Markus Hecker.

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Ben Mansour, H., Houas, I., Montassar, F. et al. Alteration of in vitro and acute in vivo toxicity of textile dyeing wastewater after chemical and biological remediation. Environ Sci Pollut Res 19, 2634–2643 (2012). https://doi.org/10.1007/s11356-012-0802-7

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  • DOI: https://doi.org/10.1007/s11356-012-0802-7

Keywords

  • Textile wastewater
  • Pseudomonas putida mt-2
  • Biodegradation
  • Genotoxicity
  • Lipoperoxidation
  • Cholinesterase activities