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Phytoremediation of a sulphonated azo dye Green HE4B by Glandularia pulchella (Sweet) Tronc. (Moss Verbena)

Abstract

Purpose

The dyes and dye stuffs present in effluents released from textile dyeing industries are potentially mutagenic and carcinogenic. Phytoremediation technology can be used for remediating sites contaminated with such textile dyeing effluents. The purpose of the work was to explore the potential of Glandularia pulchella (Sweet) Tronc. to decolorize different textile dyes, textile dyeing effluent, and synthetic mixture of dyes.

Methods

Enzymatic analysis of the plant roots was performed before and after decolorization of dye Green HE4B. Analysis of the metabolites of Green HE4B degradation was done using UV–Vis spectroscopy, high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR), and gas chromatography–mass spectroscopy (GC-MS). The ability of the plant to decolorize and detoxify a textile dyeing effluent and a synthetic mixture of dyes was studied by a determination of the American Dye Manufacturer’s Institute (ADMI), biological oxygen demand (BOD), and chemical oxygen demand (COD). Phytotoxicity studies were performed.

Result

Induction of the activities of lignin peroxidase, laccase, tyrosinase, and 2,6-dichlorophenol indophenol reductase was obtained, suggesting their involvement in the dye degradation. UV–Vis spectroscopy, HPLC, and FTIR analysis confirmed the degradation of the dye. Three metabolites of the dye degradation were identified, namely, 1-(4-methylphenyl)-2-{7-[(Z)-phenyldiazenyl] naphthalen-2-yl} diazene; 7,8-diamino-2-(phenyldiazenyl) naphthalen-1-ol; and (Z)-1,1′-naphthalene-2,7-diylbis (phenyldiazene) using GC-MS. ADMI, BOD, and COD values were reduced. The non-toxic nature of the metabolites of Green HE4B degradation was revealed by phytotoxicity studies.

Conclusion

This study explored the phytoremediation ability of G. pulchella (Sweet) Tronc. in degrading Green HE4B into non-toxic metabolites.

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References

  1. APHA (1995) Standard methods for the examination of water and wastewater, 19th edn. American Public Health Association, Washington

    Google Scholar 

  2. Aubert S, Schwitzguebel J-P (2002) Capillary elctrophoretic separation of sulphonated anthraquinones in a variety of matrices. Chromatographia 56:693–697

    Article  CAS  Google Scholar 

  3. Aubert S, Schwitzguebel JP (2004) Screening of plant species for the phytotreatment of wastewater containing sulphonated anthraquinones. Water Res 38:3569–3575

    Article  CAS  Google Scholar 

  4. Bafana A, Jain M, Agrawal G, Chakrabarti T (2009) Bacterial reduction in genotoxicity of Direct Red 28 dye. Chemosphere 74:1404–1406

    Article  CAS  Google Scholar 

  5. Carias CC, Novais JM, Martins-Dias S (2007) Phragmites australis peroxidases role in the degradation of an azo dye. Water Sci Technol 56:263–269

    Article  CAS  Google Scholar 

  6. Casieri L, Varese GC, Anastasi A, Prigione V, Svobodova K, Marchisio VF, Novotny C (2008) Decolorization and detoxification of reactive industrial dyes by immobilized fungi Trametes pubescens and Pleurotus ostreatus. Folia Microbiol 53:44–52

    Article  CAS  Google Scholar 

  7. Chen KC, Wu JY, Liou DJ, Hwang SJ (2003) Decolorization of the textile dyes by newly isolated bacterial strains. J Biotechnol 101:57–68

    Article  CAS  Google Scholar 

  8. Cluis C (2004) Junk-greedy greens: phytoremediation as a new option for soil decontamination. BioTeach J 2:61–67

    Google Scholar 

  9. Ghodake GS, Telke AA, Jadhav JP, Govindwar SP (2009) Potential of Brassica juncea in order to treat textile effluent contaminated sites. Int J Phytoreme 11:297–312

    Article  Google Scholar 

  10. Hatvani N, Mecs I (2001) Production of laccase and manganese peroxidase by Lentinus edodes on malt containing byproduct of the brewing process. Process Biochem 37:491–496

    Article  Google Scholar 

  11. Kagalkar AN, Jagtap UB, Jadhav JP, Bapat VA, Govindwar SP (2009) Biotechnological strategies for phytoremediation of the sulphonated azo dye Direct Red 5B using Blumea malcolmii Hook. Bioresour Technol 100:4104–4110

    Article  CAS  Google Scholar 

  12. Kagalkar AN, Jagtap UB, Jadhav JP, Govindwar SP, Bapat VA (2010) Studies on phytoremediation potentiality of Typhonium flagelliforme for the degradation of Brilliant Blue R. Planta 232:271–285

    Article  CAS  Google Scholar 

  13. Kalme SD, Parshetti GK, Jadhav SU, Govindwar SP (2007) Biodegradation of benzidine based dye Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112. Bioresour Technol 98:1405–1410

    Article  CAS  Google Scholar 

  14. Kandaswami C, Vaidyanathan CS (1973) Oxidation of catechol in plants. IV. Purification and properties of the 3,4,3′,4′-tetrahydroxydiphenyl forming enzyme system from Tecoma leaves. J Biol Chem 248:4035–4039

    CAS  Google Scholar 

  15. Kao CM, Chou MS, Fang WL, Liu BW, Huang BR (2001) Regulating colored textile wastewater by 3/31 wavelength ADMI methods in Taiwan. Chemosphere 44:1055–1063

    Article  CAS  Google Scholar 

  16. Nilratnisakorn S, Thiravetyan P, Nakbanpote W (2007) Synthetic reactive dye wastewater treatment by narrow-leaved cattails (Typha angustifolia Linn.): effects of dye, salinity and metals. Sci Total Environ 384:67–76

    Article  CAS  Google Scholar 

  17. Novotny CK, Dias N, Kapanen A, Malachova K, Vandrovcova M, Itavaara M, Lima N (2006) Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes. Chemosphere 63:1436–1442

    Article  CAS  Google Scholar 

  18. Patil P, Desai N, Govindwar SP, Jadhav JP, Bapat VA (2009) Degradation analysis of Reactive Red 198 by hairy roots of Tagetes patula L. (Marigold). Planta 230:725–735

    Article  CAS  Google Scholar 

  19. Raghukumar C, Chandramohan D, Jr M, Reddy CA (1996) Degradation of lignin and decolourization of paper mill bleach plant effluent (BPE) by marine fungi. Biotechnol Lett 18:105–106

    Article  CAS  Google Scholar 

  20. Robinson T, McMullan G, Marchant R, Nigam P (2001) Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresour Technol 77:247–255

    Article  CAS  Google Scholar 

  21. Salokhe MD, Govindwar SP (1999) Effect of carbon source on the biotransformation enzymes in Serratia marcescens. World J Microbiol Biotechnol 15:229–232

    Article  CAS  Google Scholar 

  22. Shanmugam V, Kumari M, Yadav K (1999) n-Propanol as a substrate for assaying the lignin peroxidase activity of Phanerochaete chrysoporium. Ind J Biochem Biophys 36:39–43

    CAS  Google Scholar 

  23. Sparado JT, Gold MH, Renganathan V (1992) Degradation of azo dyes by lignin degrading fungus Penicillium chrysosporium. Appl Environ Microbiol 58:2397–2401

    Google Scholar 

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Acknowledgments

A. N. Kabra, R. V. Khandare, and M. B. Kurade wish to thank the Department of Biotechnology (DBT), New Delhi, India, for providing Junior Research Fellowship.

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Correspondence to Sanjay P. Govindwar.

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Responsible editor: Elena Maestri

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Kabra, A.N., Khandare, R.V., Kurade, M.B. et al. Phytoremediation of a sulphonated azo dye Green HE4B by Glandularia pulchella (Sweet) Tronc. (Moss Verbena). Environ Sci Pollut Res 18, 1360–1373 (2011). https://doi.org/10.1007/s11356-011-0491-7

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Keywords

  • Phytoremediation
  • Glandularia pulchella (Sweet) Tronc.
  • GreenHE4B
  • Decolorization
  • Textile dyeing effluent