Abstract
Background, aim, and scope
For many years, highly concentrated wastewater generated from the pulp and paper industry has become the focus of much concern worldwide. The objectives of this study were to determine the treatment efficiency of the alkaline peroxide mechanical pulp (APMP) process wastewater using a sequencing batch reactor (SBR) and analyze the microbial dynamics of the wastewater treatment system using the random amplified polymorphic DNA (RAPD) method.
Materials and methods
An SBR was applied to the treatment of APMP pulp and paper process wastewater. The wastewater characteristics and many physicochemical operator indicators in the wastewater treatment process were analyzed and determined according to standard methods. Microbial 16 S rDNA in active sludge was extracted, amplified, and analyzed using the RAPD method for the microbial dynamics of the wastewater treatment system.
Results and discussion
Ten kinds of natural organic compounds of plants such as monoterpene were detected in the APMP pulp and paper process wastewater. With an influent chemical oxygen demand (COD) that varied in the range of 685.7 to 907.5 mg/L, the corresponding effluent COD was 176.5 to 266.1 mg/L and the removal efficiency was 70.3% to 79.8%. An optimal strain (S308: CAGGGGTGGA) was selected to study the population dynamics and diversity of the bacterial community. The RAPD-polymerase chain reaction (PCR) fingerprints showed very high polymorphism of the genetic bands (78–100%). Four groups of species were clustered using the unweighted pair group method with arithmetic (UPGMA) analysis, and the genetic distance was close between the species within each group. The Shannon–Weaver index was high and varied over time with the COD removal.
Conclusions
The RAPD-PCR technique can be used to study microbial dynamics, which was shown to vary over time with the removal efficiency of SBR treating APMP pulp and paper process wastewater.
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References
Alves L, Marques S, Matos J, Tenreiro R, Gírio FM (2008) Dibenzothiophene desulphurization by Gordonia alkanivorans strain 1B using recycled paper sludge hydrolyze. Chemosphere 70:967–973
Becker PM, Wand H, Martius GG, Weissbrodt E, Stottmeister U (1998) Functional and structural successions in arbitrary samples of heterotrophic bacteria during aerobic treatments of lignite-carbonization wastewater in in situ enclosures. Can J Microbiol 44:211–220
Beun JJ, Hendriks A, Loosdrecht MCM, Morgenroth E, Wilderer PA, Heijnen JJ (1999) Aerobic granulation in a sequencing batch reactor. Water Res 33:2283–2290
Camarero S, Sarkar S, Francisco JRD, María JM, MÁngel T (1999) Description of a versatile peroxides involved in the natural degradation of lignin that has both manganese peroxides and lignin peroxides substrate interaction sites. J Biol Chem 274:10324–10330
Choi JH, Lee SH, Fukushi K, Kazuo Y (2007) Comparison of sludge characteristics and PCR-DGGE based microbial diversity of nano-filtration and micro-filtration membrane bioreactors. Chemosphere 67:1543–1550
Das IK, Fakrudinand B, Arora DK (2008) RAPD cluster analysis and chlorate sensitivity of some Indian isolates of Macrophomina phaseolina from sorghum and their relationships with pathogenicity. Res Microbio 163:215–224
Diao HF, Li XY, Gu JD, Shi HC, Xie ZM (2004) Electron microscopic investigation of the bactericidal action of electrochemical disinfection in comparison with chlorination, ozonation and Fenton reaction. Process Biochem 39:1421–1426
EPA (2002) Analyses in water and wastewater, 4th edn. Environmental Science Press, Beijing
Franta JR, Wilderer PA (1997) Biological treatment of papermill wastewater by sequencing batch reactor technology to reduce residual organics. Water Sci Technol 35:129–136
Fulthorpe RR, Allen DG (1995) A comparison of organochlorine removal from bleached kraft pulp and paper-mill effluents by dehalogenating Pseudomonas, Ancylobacter and Methylobacterium strains. Appl Microbiol Biotechnol 42:782–789
Hardrys H, Balick M, Schier W (1992) Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol Ecol 1:55–63
Luxmy BS, Nakajima F, Yamamoto K (2000) Analysis of bacterial community in membrane-separation bioreactors by fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) techniques. Water Sci Technol 41:259–268
Martín-Cereceda M, Serrano S, Guinea A (2006) A comparative study of ciliated protozoa communities in active-sludge plants. FEMS Microbiol Ecol 21:267–276
Misra G, Pavlostathis SG, Perdue EM, Araujo R (1996) Aerobic biodegradation of selected monoterpenes. Appl Microbiol Biotechnol 45:831–838
Pokhrel D, Viraraghavan T (2004) Treatment of pulp and paper mill wastewater—a review. Sci Total Environ 333:37–58
Ronimus RS, Morgan HW, Parker LE (1997) The utilization of RAPD-PCR for identifying thermophilic and mesophilic Bacillus species. FEMS Microbiol Lett 147:75–79
Sang TS, Kenichi T (2005) Genotypic characterization of Burkholderia cenocepacia strains by rep-PCR and PCR-RFLP of the fliC gene. FEMS Microbiol Lett 245:19–24
Seppola M, Olsen RE, Sandaker E, Kanapathippillai P, Holzapfel W, Ringø E (2006) Random amplification of polymorphic DNA (RAPD) typing of carnobacteria isolated from hindgut chamber and large intestine of Atlantic cod (Gadus morhua L.). Syst Appl Microbiol 29:131–137
Spellerberg IF, Fedor PJ (2003) A tribute to Claude Shannon (1916–2001) and a plea for more rigorous use of species richness, species diversity and the ‘Shannon-Wiener’ Index. Global Ecol Biogeogr 12:177–179
Svenson A, Sandén B, Dalhammar G, Remberger M, Kaj L (2000) Toxicity identification and evaluation of nitrification inhibitors in wastewaters. Environ Toxicol 15:527–532
Swaileh KM, Hussein R, Ezzughayyar A (2008) Evaluating wastewater-induced plant genotoxicity using randomly amplified polymorphic DNA. Environ Toxicol 23:117–122
Tiirola MA, Suvilampi JE, Kulomaa MS, Rintala JA (2003) Microbial diversity in a thermophilic aerobic biofilm process: analysis by length heterogeneity PCR (LH-PCR). Water Res 37:2259–2268
Tripathi CS, Allen DG (1999) Comparison of mesophilic and thermophilic aerobic biological treatment in sequencing batch reactors treating bleached kraft pulp mill effluent. Water Res 33:836–846
Tsang YF, Hua FL, Chua H, Sin SN, Wang YJ (2007) Optimization of biological treatment of paper mill effluent in a sequencing batch reactor. Biochem Eng J 34:193–199
Werker AG, Becker J, Huitema C (2003) Assessment of active sludge microbial community analysis in full-scale biological wastewater treatment plants using patterns of fatty acid isopropyl esters (FAPEs). Water Res 37:2162–2172
Wu J, Xiao YZ, Yu H (2005) Degradation of lignin in pulp mill wastewaters by white-rot fungi on biofilm. Biores Technol 96:1357–1363
Zhang XL, Yan X, Gao PP, Wang LH, Zhou ZH, Zhao LP (2005) Optimized sequence retrieval from single bands of temperature gradient gel electrophoresis profiles of the amplified 16 S rDNA fragments from an active sludge system. J Microbiol Methods 60:1–11
Zhang XX, Jia HY, Wu B, Zhao DY, Li WX, Cheng SP (2009) Genetic analysis of protoplast fusant Xhhh constructed for pharmaceutical wastewater treatment. Bioresour Technol 100:1910–1914
Zwirglmaier K (2005) Fluorescence in situ hybridisation (FISH)—the next generation. FEMS Microbiol Lett 246:151–158
Acknowledgements
This work was supported by the National Key Science and Technology R&D Program of the 11th Five-Year Plan of China (grant no. 2006BAD18B0503).
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Zhan, P., Chen, J., He, G. et al. Microbial dynamics in a sequencing batch reactor treating alkaline peroxide mechanical pulp and paper process wastewater. Environ Sci Pollut Res 17, 1599–1605 (2010). https://doi.org/10.1007/s11356-010-0348-5
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DOI: https://doi.org/10.1007/s11356-010-0348-5