Abstract
Purpose
Contamination with petroleum hydrocarbons (PHC) is a global problem with environmental implications. Physico-chemical treatments can be used for soil cleanup, but they are expensive, and can have implications for soil structure and environment. Otherwise, biological remediation treatments are cost-effective and restore soil structure. Several remediation experiments have been carried out in the lab and in the field; however, there is the challenge to achieve as good or better results in the field as in the laboratory. In the ambit of a project aiming at investigating suitable biological remediation approaches for recovering a refinery contaminated soil, we present here results obtained in bioremediation trials. The approaches biostimulation and bioaugmentation were tested, in parallel, and compared with natural attenuation. For this purpose, mesocosm experiments were carried out inside the refinery area, which constitutes a real asset of this work.
Methods
Soil contaminated with crude oil was excavated, re-contaminated with turbine oil, homogenised and used to fill several 0.5 m3 high-density polyethylene containers. The efficiency of procedures as follows: (1) natural attenuation; (2) manual aeration; (3) biostimulation by adding (3.1) only nutrients; and (3.2) nutrients and a non-ionic surfactant; and (4) bioaugmentation in the presence of added (4.1) nutrients or (4.2) nutrients and a non-ionic surfactant were evaluated after a 9-month period of experiment. For bioaugmentation, a commercial bacterial product was used. In addition to physico-chemical characterization, initial and final soil contents in total petroleum hydrocarbons (TPH) (by Fourier transform infrared spectrophotometry) and the total number of bacteria (by total cell counts) were carried out. For TPH degradation evaluation the soil was divided in four fractions corresponding to different depths: 0–5; 5–10; 10–15; and 15–20 cm. Mean values of percentages of PHC degradation varied between 20 and 50% at surface and between 10 and 35% below 5-cm depth. Natural attenuation was as efficient as most of the tested treatments (about 30% TPH degradation) being exceeded only by bioaugmentation combined with nutrient and surfactant amendments (about 50% TPH degradation). Higher TPH degradation at surface suggests that a combination of sufficient dioxygen, propitious for aerobically degradation, with sunlight required for production of strong photochemical oxidants like ozone, contributed for enhancing degradation. Indeed, the atmosphere of the refineries is relatively rich in volatile organic compounds and nitrogen dioxide (a side-product of the combustion of residual volatile PHC released by the chimneys), which are precursors of O3 and other photochemical oxidants produced in sunny days, which are very common in Portugal. The fact that natural attenuation was as efficient as most of the soil treatments tested was very probably a result of the presence, in the initial soil, of physiologically adapted native microorganisms, which could be efficient in degrading PHC.
Conclusions
A cost-effective way to reduce half-life for the degradation of PHC of contaminated soil of the refinery will be a periodic revolving of the soil, like tillage, in order to expose to the oxidative atmosphere the different layers of contaminated soil. A combination of soil revolving with bioaugmentation together with nutrients and surfactant amendments may result in an additional improvement of PHC degradation rate. However, this last procedure will raise markedly the price of the remediation treatment.
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References
Bento FM, Camargo FAO, Okeke BC, Frankenberger WT (2005) Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresour Technol 96(9):1049–1055
Del’Arco JP, de França FP (1999) Biodegradation of crude oil in sandy sediment. Int Biodeter Biodegr 44(2–3):87–92
Devinny JS, Chang S-H (2000) Bioaugmentation for soil remediation. In: Wise DL, Trantolo DJ, Cichon EJ, Inyang HI, Stottmeister U (eds) Bioremediation of contaminated soils. Marcel Dekker, Inc., New York, pp 465–488
El Fantroussi S, Agathos SN (2005) Is bioaugmentation a feasible strategy for pollutant removal and site remediation? Curr Opin Microbiol 8(3):268–275
Gogoi BK, Dutta NN, Goswami P, Krishna Mohan TR (2003) A case study of bioremediation of petroleum-hydrocarbon contaminated soil at a crude oil spill site. Adv Environ Res 7(4):767–782
Haigh SD (1996) A review of the interaction of surfactants with organic contaminants in soil. Sci Total Environ 185(1–3):161–170
Hamdi H, Benzarti S, Manusadzianas L, Aoyama I, Jedidi N (2007) Bioaugmentation and biostimulation effects on PAH dissipation and soil ecotoxicity under controlled conditions. Soil Biol Biochem 39(8):1926–1935
Jørgensen KS, Puustinen J, Suortti AM (2000) Bioremediation of petroleum hydrocarbon-contaminated soil by composting in biopiles. Environ Pollut 107(2):245–254
Kepner RL Jr, Pratt JR (1994) Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. Microbiol Rev 58:603–615
Klimkowicz-Pawlas A, Maliszewska-Kordybach B (2008) Effect of the selected organic solvents on the activity of soil microorganisms. Rocz Panstw Zakl Hig 59(1):83–96
Lee S-H, Lee W-S, Lee C-H, Kim J-G (2008) Degradation of phenanthrene and pyrene in rhizosphere of grasses and legumes. J Hazard Mater 153(1–2):892–898
Leverage E (2007) http://www.environmentalleverage.com/MicroSolv%20400.htm (Accessed 20 April 2009)
Liste H-H, Felgentreu D (2006) Crop growth, culturable bacteria, and degradation of petrol hydrocarbons (PHCs) in a long-term contaminated field soil. Appl Soil Ecol 31(1–2):43–52
Method 8440 (1996) Total recoverable petroleum hydrocarbons by infrared spectrophotometry, Environmental Protection Agency
Ouyang W, Liu H, Murygina V, Yu Y, Xiu Z, Kalyuzhnyi S (2005) Comparison of bio-augmentation and composting for remediation of oily sludge: a field-scale study in China. Process Biochem 40(12):3763–3768
Porter KG, Feig YS (1980) The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25:943–948
Sabaté J, Viñas M, Solanas AM (2004) Laboratory-scale bioremediation experiments on hydrocarbon-contaminated soils. Int Biodeter Biodegr 54(1):19–25
Van Veen JA, Van Overbeek LS, Van Elsas JD (1997) Fate and activity of microorganisms introduced into soil. Microbiol Mol Biol R 61(2):121–135
Vasudevan N, Rajaram P (2001) Bioremediation of oil sludge-contaminated soil. Environ Int 26(5–6):409–411
Acknowledgement
To FC&T for the Ph.D. scholarship of M. N. Couto (SFRH/31816/2006). To Refinaria do Porto (GALP Energy) for the financial support. Logistical support by J. Amorim, from Refinaria do Porto and technological advices and logistical support by I. Teixeira, R. Salé and E. Bernardes from Berka—Engº E. Bernardes, Lda and M. I. Caçador from Oceanographic Institute of the Science Faculty of Lisbon University. To A.A. Bordalo from Laboratório de Hidrobiologia, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS).
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Couto, M.N.P.F.S., Monteiro, E. & Vasconcelos, M.T.S.D. Mesocosm trials of bioremediation of contaminated soil of a petroleum refinery: comparison of natural attenuation, biostimulation and bioaugmentation. Environ Sci Pollut Res 17, 1339–1346 (2010). https://doi.org/10.1007/s11356-010-0318-y
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DOI: https://doi.org/10.1007/s11356-010-0318-y