Abstract
Introduction
The effect of oceanic CO2 sequestration was examined exposing a deep-sea bacterium identified as Vibrio alginolyticus (9NA) to elevated levels of carbon dioxide and monitoring its growth at 2,750 psi (1,846 m depth).
Findings
The wild-type strain of 9NA could not grow in acidified marine broth below a pH of 5. The pH of marine broth did not drop below this level until at least 20.8 mM of CO2 was injected into the medium. 9NA did not grow at this CO2 concentration or higher concentrations (31.2 and 41.6 mM) for at least 72 h. Carbon dioxide at 10.4 mM also inhibited growth, but the bacterium was able to recover and grow. Exposure to CO2 caused the cell to undergo a morphological change and form a dimple-like structure. The membrane was also damaged but with no protein leakage.
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Acknowledgements
The authors thank Professor Frank Millero, University of Miami, for providing the sea water equilibrium model and Dr. Carl Wirsen for the strain of 9NA. This work was supported by a grant from the Army Research Office and the Department of Chemistry and Life Science’s Undergraduate Research Program.
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Labare, M.P., Bays, J.T., Butkus, M.A. et al. The effects of elevated carbon dioxide levels on a Vibrio sp. isolated from the deep-sea. Environ Sci Pollut Res 17, 1009–1015 (2010). https://doi.org/10.1007/s11356-010-0297-z
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DOI: https://doi.org/10.1007/s11356-010-0297-z