Abstract
Background
Pulmonary artery hypertension (PAH) is a complex disorder that can lead to right heart failure. The generation of caveolin-1 deficient mice (CAV-1−/−) has provided an alternative genetic model to study the mechanisms of pulmonary hypertension. However, the vascular adaptations in these mice have not been characterized.
Objective
To determine the histological and functional changes in the pulmonary and carotid arteries in CAV-1−/− induced PAH.
Methods
Pulmonary and carotid arteries of young (4–6 months old) and mature (9–12 months old) CAV-1−/− mice were tested and compared to normal wild type mice.
Results
Artery stiffness increases in CAV-1−/− mice, especially the circumferential stiffness of the pulmonary arteries. Increases in stiffness were quantified by a decrease in circumferential stretch and transition strain, increases in elastic moduli, and an increase in total strain energy at physiologic strains. Changes in mechanical properties for the pulmonary artery correlated with increased collagen content while changes in the carotid artery correlated with decreased elastin content.
Conclusions
We demonstrated that an increase in artery stiffness is associated with CAV-1 deficiency-induced pulmonary hypertension. These results improve our understanding of arterial remodeling in PAH.
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Change history
23 November 2020
A Correction to this paper has been published: https://doi.org/10.1007/s11340-020-00675-5
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Acknowledgments
This work was partially supported by the National Institutes of Health HL095852 (HCH), NHLBI HHSN 268201000036C (N01-HV-00244) for the UTHSCSA Cardiovascular Proteomics Center, and the Janey Briscoe Center of Excellence in Cardiovascular Research (CJLS).
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This study did not involve any human subject and the use of lab animal followed a protocol which minimized the use of animal and fully considered the welfare of the animal was approved by IACUC.
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The original article was updated to include the missing section from equation (4) to equation (8). This error was introduced during production process and was not the fault of author.
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Moreno, J., Escobedo, D., Calhoun, C. et al. Arterial Wall Stiffening in Caveolin-1 Deficiency-Induced Pulmonary Artery Hypertension in Mice. Exp Mech 61, 217–228 (2021). https://doi.org/10.1007/s11340-020-00666-6
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DOI: https://doi.org/10.1007/s11340-020-00666-6