Experimental Mechanics

, Volume 55, Issue 8, pp 1441–1449 | Cite as

HAMr: A Mechanical Impactor for Repeated Dynamic Loading of In vitro Neuronal Networks

  • S. Koumlis
  • D. Buecker
  • G. Moler
  • V. Eliasson
  • P. Sengupta
Article

Abstract

A new tool for exploring the effects of repeated low-amplitude mechanical impacts onto in vitro neuronal networks is presented. The experimental setup, HAMr, is specifically designed to allow variability in impact conditions while ensuring a highly repeatable result. HAMr’s functionality to induce inflammation related to mild traumatic brain injuries has been validated by assessing its capability to induce elevated expression of the inflammatory protein IL-1 β in in vitro neuronal cell cultures. The two main results obtained for the inflammatory response in dissociated cortical networks, presented for a range of impact force amplitude and total number of impacts, can be summarized as follows. First, the results demonstrate a strong correlation between ensuing inflammation level and numbers of impact. Second, the results indicate a possible existence of a safe threshold of number of impacts that does not initiate an inflammatory response.

Keywords

In vitro Neuronal networks Dynamic loading Blunt impact Repeated impacts 

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Copyright information

© Society for Experimental Mechanics 2015

Authors and Affiliations

  • S. Koumlis
    • 1
  • D. Buecker
    • 1
  • G. Moler
    • 1
  • V. Eliasson
    • 1
  • P. Sengupta
    • 2
    • 3
  1. 1.Aerospace and Mechanical EngineeringUniversity of Southern CaliforniaLos AngelesUSA
  2. 2.ISP/Applied Sciences LaboratoryWashington State UniversityPullmanUSA
  3. 3.Department of Bioengineering and Beckman Institute for Advanced Science and TechnologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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