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The combined effect of high-intensity interval training and intermittent fasting on lipid profile and peroxidation in Wistar rats under high-fat diet



High-fat diet is considered as the main risk factor for the development of dyslipidemia and lipid peroxidation, which in turn results in serious health problems such as cardiovascular disease, type 2 diabetes, and some forms of cancers. Therefore, the purpose of this study was to investigate the combined effect of high-intensity interval training (HIIT) and intermittent fasting (IF) on lipid profile and peroxidation in Wistar rats under a high-fat diet.


A total of 28 male Wistar rats went through a high-fat diet for 12 weeks. Then, they were randomly divided into four groups: (1) HIIT (3 days week−1 for 6 weeks), (2) IF (3 days week−1 for 6 weeks), (3) combined HIIT and IF (received both treatment for 6 weeks), and (4) control (CON). All groups were under a high-fat diet until the end of the study.


According to the results, LDL levels significantly decreased in the HIIT group compared to the CON group, and in the IF and HIIT + IF groups, a non-significant increase in HDL was observed compared to the CON group. In HIIT + IF and HIIT groups, LDL/HDL ratio decreased significantly compared to the CON group (P < 0.05). Moreover, in IF group, a significant decrease in TG occurred in comparison with HIIT and HIIT + IF groups. A significant increase in serum MDA levels was observed in IF and HIIT + IF groups but not in the HIIT group in comparison with the CON group.


Our results suggest that in subjects under a high-fat diet, both HIIT and IF may help to improve lipid profile, but their combination may not have any synergistic effect. Also, IF and HIIT + IF may increase lipid peroxidation in subjects under a high-fat diet.

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High-fat diet


High-intensity interval training


Intermittent fasting


Lipid peroxidation




Cardiovascular disease


Low-density lipoprotein


High-density lipoprotein




Total cholesterol


Reactive oxygen species


Deoxyribonucleic acid


Polyunsaturated fatty acids


Dietary restriction


  1. Nakamura T, Bando H, Kawashima T (2019) Weight reduction by effective protocol of diet and exercise. J Diab Obes Metab 2(1):106

    Google Scholar 

  2. Duan Y, Zeng L, Zheng C, Song B, Li F, Kong X, Xu K (2018) Inflammatory links between high fat diets and diseases. Front Immunol 9:2649–2649.

    Article  CAS  Google Scholar 

  3. Ghibaudi L, Cook J, Farley C, van Heek M, Hwa JJ (2002) Fat Intake affects adiposity, comorbidity factors, and energy metabolism of Sprague–Dawley rats. Obes Res 10(9):956–963.

    Article  CAS  Google Scholar 

  4. Hariri N, Thibault L (2010) High-fat diet-induced obesity in animal models. Nutr Res Rev 23(2):270–299.

    Article  CAS  Google Scholar 

  5. Woods SC, Seeley RJ, Rushing PA, D’Alessio D, Tso P (2003) A controlled high-fat diet induces an obese syndrome in rats. J Nutr 133(4):1081–1087.

    Article  CAS  Google Scholar 

  6. Yang R-l, Li W, Shi Y-H, Le G-W (2008) Lipoic acid prevents high-fat diet-induced dyslipidemia and oxidative stress: a microarray analysis. Nutrition 24(6):582–588.

    Article  CAS  Google Scholar 

  7. Blagojević IP, Ignjatović S, Macut D, Kotur-Stevuljević J, Božić-Antić I, Vekić J, Bjekić-Macut J, Kastratović-Kotlica B, Andrić Z, Ilić D (2018) Evaluation of a summary score for dyslipidemia, oxidative stress and inflammation (the doi score) in women with polycystic ovary syndrome and its relationship with obesity. J Med Biochem 37(4):476.

    Article  CAS  Google Scholar 

  8. Marseglia L, Manti S, D’Angelo G, Nicotera A, Parisi E, Di Rosa G, Gitto E, Arrigo T (2015) Oxidative stress in obesity: a critical component in human diseases. Int J Mol Sci 16(1):378–400.

    Article  CAS  Google Scholar 

  9. Ighodaro OM, Akinloye OA (2018) First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid. Alex J Med 54(4):287–293.

    Article  Google Scholar 

  10. Huang C-J, McAllister MJ, Slusher AL, Webb HE, Mock JT, Acevedo EO (2015) Obesity-related oxidative stress: the impact of physical activity and diet manipulation. Sports Med Open 1(1):32.

    Article  Google Scholar 

  11. McMurray F, Patten DA, Harper M-E (2016) Reactive oxygen species and oxidative stress in obesity—recent findings and empirical approaches. Obesity 24(11):2301–2310.

    Article  CAS  Google Scholar 

  12. Ayala A, Munoz MF, Arguelles S (2014) Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014:31.

    Article  CAS  Google Scholar 

  13. Barrera G, Pizzimenti S, Daga M, Dianzani C, Arcaro A, Cetrangolo GP, Giordano G, Cucci MA, Graf M, Gentile F (2018) Lipid peroxidation-derived aldehydes, 4-hydroxynonenal and malondialdehyde in aging-related disorders. Antioxidants 7(8):102.

    Article  CAS  Google Scholar 

  14. Wilson RA, Deasy W, Stathis CG, Hayes A, Cooke MB (2018) Intermittent fasting with or without exercise prevents weight gain and improves lipids in diet-induced obese mice. Nutrients 10(3):346.

    Article  CAS  Google Scholar 

  15. Yan Z, Kronemberger A, Blomme J, Call JA, Caster HM, Pereira RO, Zhao H, de Melo VU, Laker RC, Zhang M, Lira VA (2017) Exercise leads to unfavourable cardiac remodelling and enhanced metabolic homeostasis in obese mice with cardiac and skeletal muscle autophagy deficiency. Sci Rep 7(1):7894.

    Article  CAS  Google Scholar 

  16. Gillen JB, Percival ME, Ludzki A, Tarnopolsky MA, Gibala MJ (2013) Interval training in the fed or fasted state improves body composition and muscle oxidative capacity in overweight women. Obesity 21(11):2249–2255.

    Article  CAS  Google Scholar 

  17. Costa KB, Magalhães SM, Aguiar PF, de Oliveira OV, Tossige-Gomes R, de Castro MF, Amorim FT, Rocha-Vieira E (2018) Modification of blood redox homeostasis by high-intensity interval training. React Oxyg Species 5(13):56–67.

    Article  Google Scholar 

  18. Mirghani S, Yousefi M (2015) The effect of interval recovery periods during HIIT on liver enzymes and lipid profile in overweight women. Sci Sports 30(3):147–154.

    Article  Google Scholar 

  19. Astorino TA, Edmunds RM, Clark A, King L, Gallant RA, Namm S, Fischer A, Wood KM (2017) High-intensity interval training increases cardiac output and VO2max. Med Sci Sports Exerc 49(2):265–273.

    Article  Google Scholar 

  20. Airin S, Linoby A, Zaki MSM, Baki H, Sariman H, Esham B, Azam MZM, Mohamed MN (2014) The effects of high-intensity interval training and continuous training on weight loss and body composition in overweight females. In: Proceedings of the international colloquium on sports science, exercise, engineering and technology 2014 (ICoSSEET 2014) Springer, Singapore, pp 401–409.

  21. Urso ML, Clarkson PM (2003) Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189(1):41–54.

    Article  CAS  Google Scholar 

  22. Anton SD, Moehl K, Donahoo WT, Marosi K, Lee SA, Mainous AG III, Leeuwenburgh C, Mattson MP (2018) Flipping the metabolic switch: understanding and applying the health benefits of fasting. Obesity 26(2):254–268.

    Article  Google Scholar 

  23. Tinsley GM, La Bounty PM (2015) Effects of intermittent fasting on body composition and clinical health markers in humans. Nutr Rev 73(10):661–674.

    Article  Google Scholar 

  24. de Azevedo FR, Ikeoka D, Caramelli B (2013) Effects of intermittent fasting on metabolism in men. Revista da Associação Médica Brasileira (English Edition) 59(2):167–173.

    Article  Google Scholar 

  25. Mattson MP, Wan R (2005) Beneficial effects of intermittent fasting and caloric restriction on the cardiovascular and cerebrovascular systems. J Nutr Biochem 16(3):129–137.

    Article  CAS  Google Scholar 

  26. Boardley D, Fahlman M, Topp R, Morgan AL, McNevin N (2007) The impact of exercise training on blood lipids in older adults. Am J Geriatr Cardiol 16(1):30–35.

    Article  Google Scholar 

  27. Delwing-de Lima D, Ulbricht ASSF, Werlang-Coelho C, Delwing-Dal Magro D, Joaquim VHA, Salamaia EM, de Quevedo SR, Desordi L (2018) Effects of two aerobic exercise training protocols on parameters of oxidative stress in the blood and liver of obese rats. J Physiol Sci 68(5):699–706.

    Article  CAS  Google Scholar 

  28. Harvie M, Howell A (2017) Potential benefits and harms of intermittent energy restriction and intermittent fasting amongst obese, overweight and normal weight subjects—a narrative review of human and animal evidence. Behav Sci 7(1):4.

    Article  Google Scholar 

  29. Cerqueira FM, Cunha FMd, Silva CCCd, Chausse B, Romano RL, Garcia CCM, Colepicolo P, Medeiros MHG, Kowaltowski AJ (2011) Long-term intermittent feeding, but not caloric restriction, leads to redox imbalance, insulin receptor nitration, and glucose intolerance. Free Radic Bio Med 51(7):1454–1460.

    Article  CAS  Google Scholar 

  30. Chausse B, Vieira-Lara MA, Sanchez AB, Medeiros MH, Kowaltowski AJ (2015) Intermittent fasting results in tissue-specific changes in bioenergetics and redox state. PLoS ONE 10(3):e0120413.

    Article  CAS  Google Scholar 

  31. Machado MV, Vieira AB, da Conceição FG, Nascimento AR, da Nóbrega ACL, Tibirica E (2017) Exercise training dose differentially alters muscle and heart capillary density and metabolic functions in an obese rat with metabolic syndrome. Exp Physiol 102(12):1716–1728.

    Article  CAS  Google Scholar 

  32. Nurmasitoh T, Utami SY, Kusumawardani E, Najmuddin AA, Fidianingsih I (2018) Intermittent fasting decreases oxidative stress parameters in Wistar rats (Rattus norvegicus). Univ Med 37(1):31–38.

    Article  CAS  Google Scholar 

  33. Racil G, Ounis OB, Hammouda O, Kallel A, Zouhal H, Chamari K, Amri M (2013) Effects of high vs. moderate exercise intensity during interval training on lipids and adiponectin levels in obese young females. Eur J Appl Physiol 113(10):2531–2540.

    Article  CAS  Google Scholar 

  34. Herron KL, Vega-Lopez S, Conde K, Ramjiganesh T, Roy S, Shachter NS, Fernandez ML (2002) Pre-menopausal women, classified as hypo-or hyper-responders, do not alter their LDL/HDL ratio following a high dietary cholesterol challenge. J Am Coll Nutr 21(3):250–258.

    Article  Google Scholar 

  35. Tofighi A, Alizadeh R, Tolouei Azar J (2017) The effect of eight weeks high intensity interval raining (HIIT) on serum amounts of FGF21 and irisin in sedentary obese women. J Urm Univ Med Sci 28(7):453–466

    Google Scholar 

  36. Patterson RE, Laughlin GA, LaCroix AZ, Hartman SJ, Natarajan L, Senger CM, Martínez ME, Villaseñor A, Sears DD, Marinac CR (2015) Intermittent fasting and human metabolic health. J Acad Nutr Diet 115(8):1203–1212.

    Article  Google Scholar 

  37. Zhang J-j, Huang Y-j, Ke B, Liu L-p, Shangguan J-j, Meng J, Qin J (2015) Effect of alternate-day fasting therapy combined with Linggui Zhugan Decoction on hepatic oxidative stress in hyperlipidemic rat. Chin J Integr Med 3(1):6.

    Article  Google Scholar 

  38. Bhutani S, Klempel MC, Kroeger CM, Trepanowski JF, Varady KA (2013) Alternate day fasting and endurance exercise combine to reduce body weight and favorably alter plasma lipids in obese humans. Obesity 21(7):1370–1379.

    Article  CAS  Google Scholar 

  39. Plavsic L, Knezevic OM, Sovtic A, Minic P, Vukovic R, Mazibrada I, Stanojlovic O, Hrncic D, Rasic-Markovic A, Macut D (2019) Effects of high-intensity interval training and nutrition advice on cardiometabolic markers and aerobic fitness in adolescent girls with obesity. Appl Physiol Nutr Metab.

    Article  Google Scholar 

  40. Leon AS, Sanchez OA (2001) Response of blood lipids to exercise training alone or combined with dietary intervention. Med Sci Sports Exerc 33(6):S502–S515.

    Article  CAS  Google Scholar 

  41. Kelley GA, Kelley KS, Roberts S, Haskell W (2012) Combined effects of aerobic exercise and diet on lipids and lipoproteins in overweight and obese adults: a meta-analysis. J Obes.

    Article  Google Scholar 

  42. Kelley GA, Kelley KS, Roberts S, Haskell W (2012) Comparison of aerobic exercise, diet or both on lipids and lipoproteins in adults: a meta-analysis of randomized controlled trials. Clin Nutr 31(2):156–167.

    Article  CAS  Google Scholar 

  43. Real-Hohn A, Navegantes C, Ramos K, Ramos-Filho D, Cahuê F, Galina A, Salerno VP (2018) The synergism of high-intensity intermittent exercise and every-other-day intermittent fasting regimen on energy metabolism adaptations includes hexokinase activity and mitochondrial efficiency. PLoS ONE 13(12):e0202784.

    Article  CAS  Google Scholar 

  44. Ramez M, Nasirinezhad F, Rajabi H, Aboutaleb N, Naderi N (2019) Short-term exercise training increases plasma levels of klotho and total antioxidant capacity in male Wistar rats. J Shahrekord Univ Med Sci 21(1):25–30.

    Article  Google Scholar 

  45. Emami A-M, Homaee HM, Azarbayjani MA (2016) Effects of high intensity interval training and curcumin supplement on glutathione peroxidase (GPX) activity and malondialdehyde (MDA) concentration of the liver in STZ induced diabetic rats. Iran J Diabetes Obes 8(3):129–134

    Google Scholar 

  46. Walsh ME, Shi Y, Van Remmen H (2014) The effects of dietary restriction on oxidative stress in rodents. Free Radic Biol Med 66:88–99.

    Article  CAS  Google Scholar 

  47. Aydin C, Ince E, Koparan S, Cangul IT, Naziroglu M, Ak F (2007) Protective effects of long term dietary restriction on swimming exercise-induced oxidative stress in the liver, heart and kidney of rat. Cell biochem & funct 25:129-137.

    Article  CAS  Google Scholar 

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Correspondence to Ali Samadi.

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All steps of the study were performed in accordance with “Guiding Principles for the Care and Use of Research Animals” approved by the Ethical Committee of Baqiyatallah University of Medical Sciences with a code number of IR.BMSU.REC.1398.022.

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Abbasi, B., Samadi, A. & Bazgir, B. The combined effect of high-intensity interval training and intermittent fasting on lipid profile and peroxidation in Wistar rats under high-fat diet. Sport Sci Health 16, 645–652 (2020).

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