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Sport Sciences for Health

, Volume 14, Issue 2, pp 387–392 | Cite as

Effect of carbohydrates versus carbohydrates plus proteins and antioxidants on oxidative stress and muscle damage induced by single bout resistance exercise

  • Daniel dos Santos Ferreira
  • Lydiane Tavares Toscano
  • Tayse Guedes Cabral
  • Gilberto Santos Cerqueira
  • Ana Carla Lima de França
  • Alexandre Sérgio Silva
Original Article
  • 86 Downloads

Abstract

Although nutritional supplementation is controversial in the literature, the ergogenic value attributed to carbohydrate supplementation is recognized by delaying fatigue in resistance exercises. The aim of this study was to investigate the effect of supplementation of a commercial carbohydrate compound plus protein and antioxidants (CPA) in muscle damage and oxidative stress induced by a single resistance training session. Ten healthy young subjects (24 ± 4 years; 23.2 ± 1 kg/m2; \( V{\text{O}}_{2\hbox{max} } \) 44.9 ± 10 ml/kg/min) performed three series of ten exercises until concentric failure, randomly ingesting water (WAT), isolated carbohydrates (CHO) or carbohydrates associated with proteins and antioxidants (CPA). Blood samples were taken before, immediately and 24 h after each exercise session for analysis of muscle damage markers, creatine kinase (CK) and malondialdehyde (MDA) oxidative stress. Blood glucose was measured before, during and after the end of the exercises. CHO or CPA resulted in significant increases in glucose of 24 and 9.5%, respectively, at post-exercise compared to pre-exercise values. WAT resulted in a significant post-exercise increase in CK (107.6 ± 34.9–227.4 ± 82.2 U/l; p = 0.02), while CHO promoted no significant increase (226 ± 90–318 ± 190 U/l; p = 0.02) and CPA did not promote a significant reduction (130 ± 125–121 ± 83 U/l). CHO or CPA did not affect the concentration of lipid peroxidation. This study revealed that ingestion of CHO or CPA during resistance exercise decreases muscle damage, but does not influence the lipidic peroxidation marker MDA.

Keywords

Oxidative stress Carbohydrates Proteins Resistance exercise 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

The study was approved by the ethics committee and they also state that the research reported in the paper was undertaken in compliance with the Helsinki Declaration and the International Principles governing research on animals.

Informed consent

Informed consent was obtained from all participants included in the study.

References

  1. 1.
    Harger-Domitrovich SG, McClaughry AE, Gaskill SE, Ruby BC (2007) Exogenous carbohydrate spares muscle glycogen in men and women during 10 h of exercise. Med Sci Sports Exerc 39:2171–2179CrossRefPubMedGoogle Scholar
  2. 2.
    Patterson SD, Gray SC (2007) Carbohydrate gel supplementation and endurance performance during intermittent high-intensity shuttle running. Int J Sport Nutr Exerc Metab 20:445–455CrossRefGoogle Scholar
  3. 3.
    Betts JA, Williams C (2010) Short-term recovery from prolonged exercise: exploring the potential for protein ingestion to accentuate the benefits of carbohydrate supplements. Sports Med 40:941–959CrossRefPubMedGoogle Scholar
  4. 4.
    Jensen J, Rustad PI, Kolnes AJ, Lai YC (2011) The role of skeletal muscle glycogen breakdown for regulation of insulin sensitivity by exercise. Front Physiol 30:112Google Scholar
  5. 5.
    Roy BD, Tarnopolsky MA (1998) Influence of differing macronutrient intakes on muscle glycogen resynthesis after resistance exercise. J Appl Physiol 84:890–896CrossRefPubMedGoogle Scholar
  6. 6.
    Haff GG, Lehmkuhl MJ, McCoy LB, Stone MH (2003) Carbohydrate supplementation and resistance training. J Strength Cond Res 17:187–196PubMedGoogle Scholar
  7. 7.
    Chromiak JA, Smedley B, Carpenter W, Brown R, Koh YS, Lamberth JG (2004) Effect of a 10-week strength training program and recovery drink on body composition, muscular strength and endurance, and anaerobic power and capacity. Nutrition 20:420–427CrossRefPubMedGoogle Scholar
  8. 8.
    Schumm SR, Triplett NT, McBride JM, Dumke CL (2008) Hormonal response to carbohydrate supplementation at rest and after resistance exercise. Int J Sport Nutr Exerc Metab 18:260–280CrossRefPubMedGoogle Scholar
  9. 9.
    Quirino ELO, Gonçalves MCR, Oliveira CVC, Santos EP, Silva AS (2012) Influence of carbohydrate supplementation during resistance training on concentrations of the hormones cortisol and insulin. Sport Sci Health 7:93–97CrossRefGoogle Scholar
  10. 10.
    Tang JE, Manolakos JJ, Kujbida GW, Lysecki PJ, Moore DR, Phillips SM (2007) Minimal whey protein with carbohydrate stimulates muscle protein synthesis following resistance exercise in trained young men. Appl Physiol Nutr Metab 32:1132–1138CrossRefPubMedGoogle Scholar
  11. 11.
    Witard OC, Cocke TL, Ferrando AA, Wolfe RR, Tipton KD (2010) Increased net muscle protein balance in response to simultaneous and separate ingestion of carbohydrate and essential amino acids following resistance exercise. Appl Physiol Nutr Metab 39:329–339CrossRefGoogle Scholar
  12. 12.
    Bird SP, Tarpenning KM, Marino FE (2006) Independent and combined effects of liquid carbohydrate/essential amino acid ingestion on hormonal and muscular adaptations following resistance training in untrained men. Eur J Appl Physiol 97:225–238CrossRefPubMedGoogle Scholar
  13. 13.
    Kreider RB, Earnest CP, Lundberg J, Rasmussen C, Greenwood M, Cowan P, Almada AL (2007) Effects of ingesting protein with various forms of carbohydrate following resistance-exercise on substrate availability and markers of anabolism, catabolism, and immunity. J Int Soc Sports Nutr 4:18CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Baty JJ, Hwang H, Ding Z, Bernard JR, Wang B, Kwon B, Ivy JL (2007) The effect of a carbohydrate and protein supplement on resistance exercise performance, hormonal response, and muscle damage. J Strength Cond Res 21:321–329PubMedGoogle Scholar
  15. 15.
    Samadi A, Gaeini AA, Kordi MR, Rahimi M, Rahnama N, Bambaeichi EJ (2012) Effect of various ratios of carbohydrate–protein supplementation on resistance exercise-induced muscle damage. Sports Med Phys Fitness 52:151–157Google Scholar
  16. 16.
    Bird SP, Tarpenning KM, Marino FE (2006) Liquid carbohydrate/essential amino acid ingestion during a short-term bout of resistance exercise suppresses myofibrillar protein degradation. Metabolism 55:570–577CrossRefPubMedGoogle Scholar
  17. 17.
    Benedini S, Dozio E, Invernizzi PL, Vianello E, Banfi G, Terruzzi I, Luzi L, Corsi Romanelli MM (2017) Irisin: a potential link between physical exercise and metabolism—an observational study in differently trained subjects, from elite athletes to sedentary people. J Diabetes Res 1039161:1–7CrossRefGoogle Scholar
  18. 18.
    Kruse R, Vienberg SG, Vind BF, Andersen B, Højlund K (2017) Effects of insulin and exercise training on FGF21, its receptors and target genes in obesity and type 2 diabetes. Diabetologia 60(10):2042–2051CrossRefPubMedGoogle Scholar
  19. 19.
    Bloomer RJ, Goldfarb AH, McKenzie MJ, You T, Nguyen L (2004) Effects of antioxidant therapy in women exposed to eccentric exercise. Int J Sport Nutr Exerc Metab 14:377–388CrossRefPubMedGoogle Scholar
  20. 20.
    Bryer SC, Goldfarb AH (2006) Effect of high dose vitamin C supplementation on muscle soreness, damage, function, and oxidative stress to eccentric exercise. Int J Sport Nutr Exerc Metab 16:270–280CrossRefPubMedGoogle Scholar
  21. 21.
    Fleck SJ, Kramer JW (1999) Fundamentos do treinamento de força muscular. Artmed, Porto AlegreGoogle Scholar
  22. 22.
    Gibson RS (1990) Principles of nutritional assessment. Oxford University, New YorkGoogle Scholar
  23. 23.
    Delavier F (2006) Guia dos Movimentos de Musculação, 4ª edn. Manole, São PauloGoogle Scholar
  24. 24.
    Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358CrossRefPubMedGoogle Scholar
  25. 25.
    American College Sports American College of Sports Medicine (2003) Diretrizes do ACSM para os testes de esforço e sua prescrição, 6ª ed edn. Guanabara-Koogan, Rio de JaneiroGoogle Scholar
  26. 26.
    Willoughby DS, Stout JR, Wilborn CD (2007) Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength. Amino Acids 32:467–477CrossRefPubMedGoogle Scholar
  27. 27.
    Purdon C, Brousson M, Nyveen SL, Miles PDG, Halter JB, Vranic M, Marliss EB (1993) The roles of insulin and catecholamines in the glucoregulatory response during intense exercise and early recovery in insulin-dependent diabetic and control subjects. J Clin Endocrinol Metab 76:566–573PubMedGoogle Scholar
  28. 28.
    Benedini S, Longo S, Caumo A, Luzi L, Invernizzi PL (2012) Metabolic and hormonal responses to a single session of kumite (free non-contact fight) and kata (highly ritualized fight) in karate athletes. Sport Sci Health 8(2–3):81–85PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2018

Authors and Affiliations

  • Daniel dos Santos Ferreira
    • 1
  • Lydiane Tavares Toscano
    • 1
  • Tayse Guedes Cabral
    • 1
  • Gilberto Santos Cerqueira
    • 2
  • Ana Carla Lima de França
    • 3
  • Alexandre Sérgio Silva
    • 1
  1. 1.Physical Education, Laboratory of Physical Training Applied to Performance and Health, Department of Physical EducationFederal University of Paraíba (UFPB), Health Sciences Center (DEF)João PessoaBrazil
  2. 2.Post-graduation Program in Morphofunctional Sciences of the Federal University of CearaFortalezaBrazil
  3. 3.Physiotherapy, Laboratory of Physical Training Applied to Performance and Health, Department of Physical EducationFederal University of ParaibaJoao PessoaBrazil

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