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PPARγ and ADRB3 polymorphisms analysis and Irisin expression in professional water polo players

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Abstract

Background

Response to exercise is regulated by environmental factors and genetic predisposition. PPARγ and ADRB3 genes are associated with body composition. Adipose and muscle tissues secrete adipokines and myokines involved in energy expenditure; among the others, adiponectin and Irisin were associated with benefits of physical activity.

Aims

Here we investigate two common variants in PPARγ and ADRB3 genes (Pro12Ala, Trp64Arg, respectively) and the expression of Irisin in 22 professional water polo (WP) players versus 40 controls.

Methods

Anthropometric and hormonal parameters were assessed; Pro12Ala and Trp64Arg SNPs were analyzed by direct-sequencing; adiponectin and Irisin expression was evaluated through western blotting.

Results

Irisin levels were similar between WP athletes and controls. The Pro12Ala polymorphism was present in two WP players and two controls while the Trp64Arg polymorphism only in one WP player; both polymorphisms were not related to biochemical parameters, as well as to Irisin and adiponectin expression.

Conclusions

Our study suggests that the influence of two PPARγ and ADRB3 common polymorphisms on Adiponectin and Irisin expression in WP athletes is limited, if it exists at all. Further studies are needed to clarify if the positive effects mediated by physical training may pass through the regulation of Irisin, adiponectin and genetic background.

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Acknowledgements

We thank Dr. Pino Porzio and Paolo De Crescenzo, coaches of the water polo players, for their kind cooperation and also Dr. Marco Pepe, who draw blood from the players.

Author information

Author notes

  1. Olga Scudiero and Ersilia Nigro equally contributed to the work.

Authors and Affiliations

  1. CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145, Naples, Italy

    Olga Scudiero, Ersilia Nigro, Ausilia Elce, Maria Ludovica Monaco & Aurora Daniele

  2. Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131, Naples, Italy

    Olga Scudiero

  3. Università Telematica Pegaso, Naples, Italy

    Ausilia Elce & Dino Sangiorgio

  4. Department of Medicine, Surgery and Dentistry, Università degli Studi di Salerno, Baronissi, Italy

    Viviana Izzo

  5. Dipartimento di Scienze Motorie e del Benessere, Università di Napoli “Parthenope”, via Medina 40, 80133, Naples, Italy

    Pasqualina Buono

  6. Dipartimento di Medicina e di Scienze della Salute “Vincenzo Tiberio”, Università degli Studi del Molise, Campobasso, Italy

    Giovanni Villone

  7. Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Università degli Studi della Campania “Luigi Vanvitelli”, via G. Vivaldi 42, 81100, Caserta, Italy

    Aurora Daniele

Authors
  1. Olga Scudiero
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  2. Ersilia Nigro
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  3. Ausilia Elce
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  4. Viviana Izzo
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  5. Maria Ludovica Monaco
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  7. Pasqualina Buono
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  8. Giovanni Villone
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  9. Aurora Daniele
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Corresponding author

Correspondence to Aurora Daniele.

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Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the Declaration of Helsinki. All procedures were approved by the University Research Ethics Committee.

Informed consent

Informed consent was obtained from all individual participants in this study.

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Scudiero, O., Nigro, E., Elce, A. et al. PPARγ and ADRB3 polymorphisms analysis and Irisin expression in professional water polo players. Sport Sci Health 13, 395–401 (2017). https://doi.org/10.1007/s11332-017-0371-8

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  • DOI: https://doi.org/10.1007/s11332-017-0371-8

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