Abstract
Purpose
Mice can develop arterial damage and even atherosclerosis under intermittent hypoxia (IH); however, the specific mechanism of arterial damage induced by IH remains unclear. Hence, this research aimed to illustrate the underlying mechanism linking IH to arterial injury.
Materials and methods
The differential gene expression of the thoracic aorta under normoxia or IH mice was analyzed utilizing RNA sequencing. Furthermore, GO, KEGG pathway, and CIBERSORT analyses were carried out. For verification of the expression of candidate genes affected by IH, quantitative RT-qPCR (qRT-PCR) was conducted. Immunohistochemical (IHC) staining revealed immune cell infiltration in the thoracic aorta.
Results
The thickness of the intima-media of the mouse aorta was increased, and the fiber structure was disordered under IH. Transcriptomics analysis showed that in the aorta, 1137 upregulated genes and 707 downregulated genes were affected by IH, significantly related to the activation of the immune system and cell adhesion. Furthermore, B cell infiltration around the aorta was observed under IH.
Conclusions
IH might lead to structural changes in the aorta by activating the immune response and enhancing cell adhesion.
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Data availability
The datasets used during the present study are available from the corresponding author upon reasonable request.
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Funding
Hongliang Yi provided financial support in the form of the National Natural Science Foundation of China [grant numbers 81770988 and 81970869].
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Chong Xu, Haibo Ye, Jian Guan, and Hongliang Yi designed experiments. Chong Xu and Yupu Liu performed experiments and analyzed data. Xiangyu Cheng and Xiaoting Wang contributed to data collection and data interpretation. Chong Xu, Xiangyu Cheng, and Weijun Huang contributed to protocol development. Chong Xu, Jinhong Shen, and Hongliang Yi prepared the figures and wrote the manuscript.
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All procedures performed in studies involving animals were carried out following the Guide for the Care and Use of Laboratory Animals and were approved by the Animal Care and Use Committee of the Shanghai Jiao Tong University Affiliated Sixth People's Hospital.
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Xu, C., Cheng, X., Wang, X. et al. The immune response to arterial damage in a mouse model of intermittent hypoxia: a transcriptomics analysis. Sleep Breath 27, 2397–2406 (2023). https://doi.org/10.1007/s11325-023-02866-5
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DOI: https://doi.org/10.1007/s11325-023-02866-5