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Therapeutic effects and modulatory mechanism of Alpiniae oxyphyllae Fructus in chronic intermittent hypoxia induced enuresis in rats

  • Pediatrics • Original Article
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Abstract

Objective

The objective of this study was to explore the effect of Alpiniae oxyphyllae Fructus (AOF) on a rat model of chronic intermittent hypoxia (CIH)–induced enuresis. Findings of this study may help identify therapeutic targets in children with nocturnal enuresis (NE).

Methods

Female rats were randomly divided into a control group (saline gavage, 4 weeks of normal air), CIH group (saline gavage, 4 weeks of CIH), and AOF group (AOF gavage, 4 weeks of CIH). The variables measured in this study included water intake, urine output, bladder leak point pressure (BLPP), malondialdehyde (MDA) levels, and superoxide dismutase (SOD) activity. The expression levels of the purinergic P2X3 receptor, muscarinic M3 receptor, and ß3-adrenergic receptor (ß3-AR) in the bladder were also measured. The bladder was subjected to haematoxylin and eosin (HE) and Weigert staining, and histological changes were observed under a light microscope to evaluate the morphological changes in the bladder in each group.

Results

Compared with the control group, urine output was increased, and the BLPP was decreased in the CIH group, but AOF administration decreased urine output and increased BLPP. In addition, the serum MDA level increased and the SOD activity decreased in the CIH group compared with the control group. Administration of AOF decreased the MDA level and increased the SOD activity. Additionally, compared with the control group, HE and Weigert staining in the CIH group showed that the bladder detrusor muscle bundles were disordered and loose, some muscle bundles were broken, the content of collagen fibres in the gap was reduced, and the gap was significantly widened. However, following the administration of AOF, the bladder detrusor muscle bundles were neatly arranged, and the content of collagen fibres in the gap was increased. Furthermore, compared with the control group, the purinergic P2X3 receptor and muscarinic M3 receptor were expressed at higher levels, and ß3-AR was expressed at lower levels in the CIH group, but AOF administration decreased the expression of the purinergic P2X3 receptor and muscarinic M3 receptor and increased the expression of the ß3-AR.

Conclusions

AOF improves enuresis by inhibiting oxidative stress and regulating the expression of the purinergic P2X3 receptor, muscarinic M3 receptor, and ß3 adrenergic receptor.

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Funding

This study was funded by the Natural Science Foundation of China (grant number 81870075) and the Zhejiang Province Natural Science Funding Project (grant numberLY17H010003).

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Author notes

  1. Miao-Shang Su and Li Xu contributed equally to this paper and should be considered joint first authors.

Authors and Affiliations

  1. Department of Pediatric Respiratory Medicine and Sleep Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, People’s Republic of China

    Miao-Shang Su, Li Xu, Shu-Ge Gu, Nan Huang, Xi-Kai Ren, Xiao-Hong Cai & Chang-Chong Li

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  1. Miao-Shang Su
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Contributions

S-MS designed the study and edited the manuscript. XL performed the experiments, analysed data, and wrote the manuscript. G-SG, HN and R-XK performed the experiments. C-XH supervised the study and assisted with the compound test simulation cabin. L-CC supervised the study and edited the manuscript. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Chang-Chong Li.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Ethical approval was obtained from the Laboratory Animal Ethics Committee of Wenzhou Medical University (ID Number: wydw2017-0087).

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Comments

Nocturnal enuresis is a significant problem with relatively few effective remedies. In this study, the effects of a traditional Chinese medicine on bladder function were shown to be associated with helpful changes in the bladders of female rats. Perhaps not ready for Prime Time but perhaps sometime?

Ian Wilcox

NSW, Australia

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Su, MS., Xu, L., Gu, SG. et al. Therapeutic effects and modulatory mechanism of Alpiniae oxyphyllae Fructus in chronic intermittent hypoxia induced enuresis in rats. Sleep Breath 24, 329–337 (2020). https://doi.org/10.1007/s11325-019-01983-4

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  • DOI: https://doi.org/10.1007/s11325-019-01983-4

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