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Expression profile of long non-coding RNAs in rat models of OSA-induced cardiovascular disease: new insight into pathogenesis

  • Sleep Breathing Physiology and Disorders • Original Article
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Abstract

Purpose

Long non-coding RNAs (lncRNAs) are a recently identified class of regulatory molecules involved in the regulation of numerous biological processes, but their functions in a rat model of chronic intermittent hypoxia (CIH) remain largely unknown. Therefore, for further investigation, we aimed to explore lncRNA expression profiles and reveal their potential functional roles in rat models of CIH.

Methods

We used a well-established CIH rat model and conducted lncRNA microarray experiments on the heart samples of rats with CIH and under normoxia control. Differentially expressed lncRNAs and mRNAs were identified via fold-change filtering and verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatics analyses were applied to reveal the potential roles of key lncRNAs. Co-expression analysis was conducted to determine the transcriptional regulatory relationship of lncRNAs and mRNAs between the two groups.

Results

Our data indicated that 157 lncRNAs and 319 mRNAs were upregulated, while 132 lncRNAs and 428 mRNAs were downregulated in the rat model of CIH compared with sham control. Pathway analyses showed that 31 pathways involved in upregulated transcripts and 28 pathways involved in downregulated transcripts. Co-expression networks were also constructed to explore the potential roles of differentially expressed lncRNAs on mRNAs. LncRNAs, namely, XR_596701, XR_344474, XR_600374, ENSRNOT00000065561, XR_590196, and XR_597099, were validated by the use of qRT-PCR.

Conclusions

The present study first revealed lncRNAs expression profiles in a rat model of CIH, providing new insight into the pathogenesis of obstructive sleep apnea-induced cardiovascular disease.

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Funding

This study is funded by the National Natural Science Foundation of China (grant number: 81370182 and 81870074), Quanzhou Science and Technology Projects (grant number: 2018N007S), and Startup Fund for Scientific Research, Fujian Medical University (grant number: 2017XQ1102).

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Authors and Affiliations

  1. Department of Respiratory Disease, The First Affiliated Hospital of Fujian Medical University, No.20 Chazhong Road, Fuzhou, 350005, Taijiang District, China

    Qingshi Chen, Guofu Lin, Jiefeng Huang, Gongping Chen, Xiaoyun Huang & Qichang Lin

  2. The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China

    Qingshi Chen

  3. Laboratory of Respiratory Disease of Fujian Medical University, Fuzhou, China

    Guofu Lin, Jiefeng Huang, Gongping Chen, Xiaoyun Huang & Qichang Lin

  4. Fujian Provincial Sleep-Disordered Breathing Clinic Center, Fuzhou, China

    Guofu Lin, Jiefeng Huang, Gongping Chen, Xiaoyun Huang & Qichang Lin

Authors
  1. Qingshi Chen
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  2. Guofu Lin
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  3. Jiefeng Huang
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  4. Gongping Chen
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  5. Xiaoyun Huang
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Correspondence to Qichang Lin.

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All applicable guidelines for the care and use of animals were followed.

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Chen, Q., Lin, G., Huang, J. et al. Expression profile of long non-coding RNAs in rat models of OSA-induced cardiovascular disease: new insight into pathogenesis. Sleep Breath 23, 795–804 (2019). https://doi.org/10.1007/s11325-018-1753-0

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  • DOI: https://doi.org/10.1007/s11325-018-1753-0

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