Advertisement

Sleep and Breathing

, Volume 17, Issue 2, pp 867–871 | Cite as

Antioxidant vitamins prevent oxidative and carbonyl stress in an animal model of obstructive sleep apnea

  • Peter CelecEmail author
  • Ingrid Jurkovičová
  • Roman Buchta
  • Ivan Bartík
  • Roman Gardlík
  • Roland Pálffy
  • Imrich Mucska
  • Július Hodosy
Short Communication

Abstract

Purpose

The aim of our study was to analyze the effects of an antioxidant treatment on markers of oxidative and carbonyl stress in a rat model of obstructive sleep apnea.

Methods

Wistar rats were randomized into six groups—according to gender and intervention—sham, intermittent hypoxia, and intermittent hypoxia with treatment by vitamins C and E. Rats underwent tracheostomy. The tracheal cannula was closed for 12 s every minute for 1 h to simulate obstructive sleep apnea-related intermittent hypoxia. In the treatment group, rats received vitamin C and E 24 h prior to surgery.

Results

The intervention had a significant effect on advanced oxidation protein products (p = 0.008) and advanced glycation end products–specific fluorescence (p = 0.006) but no effect on malondialdehyde. Oxidation and glycation protein products were higher in intermittent hypoxia groups than in sham and in treated groups.

Conclusions

Antioxidants alleviate oxidative and carbonyl stress in an experimental model of obstructive sleep apnea. Future studies will show whether such treatment has any clinical value regarding cardiovascular complications of sleep apnea syndrome, preferably in patients with low compliance to continuous positive airway pressure.

Keywords

Sleep apnea Oxidative stress Carbonyl stress Antioxidative therapy AGEs 

Notes

Conflicts of interest

None

Disclosure of financial support

This publication is the result of the project implementation: “TRANSMED 2” ITMS: 26240120030 supported by the Research and Development Operational Program funded by the ERDF.

References

  1. 1.
    Jurkovicova I, Celec P (2004) Sleep apnea syndrome and its complications. Acta Med Austriaca 31:45–50PubMedGoogle Scholar
  2. 2.
    Jordan W, Cohrs S, Degner D, Meier A, Rodenbeck A, Mayer G, Pilz J, Ruther E, Kornhuber J, Bleich S (2006) Evaluation of oxidative stress measurements in obstructive sleep apnea syndrome. J Neural Transm 113:239–254PubMedCrossRefGoogle Scholar
  3. 3.
    Barcelo A, Barbe F, de la Pena M, Vila M, Perez G, Pierola J, Duran J, Agusti AG (2006) Antioxidant status in patients with sleep apnoea and impact of continuous positive airway pressure treatment. Eur Respir J 27:756–760PubMedCrossRefGoogle Scholar
  4. 4.
    Miyata T, Ishikawa N, van Ypersele de Strihou C (2003) Carbonyl stress and diabetic complications. Clin Chem Lab Med 41:1150–1158PubMedCrossRefGoogle Scholar
  5. 5.
    Volna J, Kemlink D, Kalousova M, Vavrova J, Majerova V, Mestek O, Svarcova J, Sonka K, Zima T (2011) Biochemical oxidative stress-related markers in patients with obstructive sleep apnea. Med Sci Monit 17:CR491–CR497PubMedCrossRefGoogle Scholar
  6. 6.
    Lam JCM, Tan KCB, Lai AYK, Lam DCL, Ip MSM (2012) Increased serum levels of advanced glycation end-products is associated with severity of sleep disordered breathing but not insulin sensitivity in non-diabetic men with obstructive sleep apnoea. Sleep Med 13:15–20PubMedCrossRefGoogle Scholar
  7. 7.
    Williams AL, Chen L, Scharf SM (2010) Effects of allopurinol on cardiac function and oxidant stress in chronic intermittent hypoxia. Sleep Breath 14:51–57PubMedCrossRefGoogle Scholar
  8. 8.
    Li CG, Lu JM, Zhang BX (2012) Development of a novel chronic intermittent hypoxia chamber. Sleep Breath 16:177–179PubMedCrossRefGoogle Scholar
  9. 9.
    Cui L, Wang JH, Wang M, Huang M, Wang CY, Xia H, Xu JG, Li MX, Wang S (2011) Injection of l-glutamate into the insular cortex produces sleep apnea and serotonin reduction in rats. Sleep Breath. doi: 10.1007/s11325-011-0586-x
  10. 10.
    Farre R, Nacher M, Serrano-Mollar A, Galdiz JB, Alvarez FJ, Navajas D, Montserrat JM (2007) Rat model of chronic recurrent airway obstructions to study the sleep apnea syndrome. Sleep 30:930–933PubMedGoogle Scholar
  11. 11.
    Celec P, Hodosy J, Behuliak M, Palffy R, Gardlik R, Halcak L, Mucska I (2012) Oxidative and carbonyl stress in patients with obstructive sleep apnea treated with continuous positive airway pressure. Sleep Breath 16:393–398PubMedCrossRefGoogle Scholar
  12. 12.
    Lavie L (2003) Obstructive sleep apnoea syndrome—an oxidative stress disorder. Sleep Med Rev 7:35–51PubMedCrossRefGoogle Scholar
  13. 13.
    Lee SD, Ju G, Choi JA, Kim JW, Yoon IY (2012) The association of oxidative stress with central obesity in obstructive sleep apnea. Sleep Breath 16:511–517PubMedCrossRefGoogle Scholar
  14. 14.
    Katalinic V, Modun D, Music I, Boban M (2005) Gender differences in antioxidant capacity of rat tissues determined by 2,2'-azinobis (3-ethylbenzothiazoline 6-sulfonate; ABTS) and ferric reducing antioxidant power (FRAP) assays. Comp Biochem Physiol C Toxicol Pharmacol 140:47–52PubMedCrossRefGoogle Scholar
  15. 15.
    Wali SO, Bahammam AS, Massaeli H, Pierce GN, Iliskovic N, Singal PK, Kryger MH (1998) Susceptibility of LDL to oxidative stress in obstructive sleep apnea. Sleep 21:290–296PubMedGoogle Scholar
  16. 16.
    Lavie L, Vishnevsky A, Lavie P (2004) Evidence for lipid peroxidation in obstructive sleep apnea. Sleep 27:123–128PubMedGoogle Scholar
  17. 17.
    Tan KC, Chow WS, Lam JC, Lam B, Bucala R, Betteridge J, Ip MS (2006) Advanced glycation endproducts in nondiabetic patients with obstructive sleep apnea. Sleep 29:329–333PubMedGoogle Scholar
  18. 18.
    Wang X, Desai K, Juurlink BHJ, de Champlain J, Wu L (2006) Gender-related differences in advanced glycation endproducts, oxidative stress markers and nitric oxide synthases in rats. Kidney Int 69:281–287PubMedCrossRefGoogle Scholar
  19. 19.
    Devi SA, Vani R, Subramanyam MV, Reddy SS, Jeevaratnam K (2007) Intermittent hypobaric hypoxia-induced oxidative stress in rat erythrocytes: protective effects of vitamin E, vitamin C, and carnitine. Cell Biochem Funct 25:221–231PubMedCrossRefGoogle Scholar
  20. 20.
    Plantinga Y, Ghiadoni L, Magagna A, Giannarelli C, Franzoni F, Taddei S, Salvetti A (2007) Supplementation with vitamins C and E improves arterial stiffness and endothelial function in essential hypertensive patients. Am J Hypertens 20:392–397PubMedCrossRefGoogle Scholar
  21. 21.
    Celec P, Jani P, Smrekova L, Mrlian A, Kudela M, Hodosy JD, Boor P, Kristova V, Jakubovsky J, Jezova D, Halcak L, Bozek P, Slamova J, Ulicna O, Hojsik D, Jurkovicova I (2003) Effects of anabolic steroids and antioxidant vitamins on ethanol-induced tissue injury. Life Sci 74:419–434PubMedCrossRefGoogle Scholar
  22. 22.
    Yokoe T, Minoguchi K, Matsuo H, Oda N, Minoguchi H, Yoshino G, Hirano T, Adachi M (2003) Elevated levels of C-reactive protein and interleukin-6 in patients with obstructive sleep apnea syndrome are decreased by nasal continuous positive airway pressure. Circulation 107:1129–1134PubMedCrossRefGoogle Scholar
  23. 23.
    Dorkova Z, Petrasova D, Molcanyiova A, Popovnakova M, Tkacova R (2008) Effects of continuous positive airway pressure on cardiovascular risk profile in patients with severe obstructive sleep apnea and metabolic syndrome. Chest 134:686–692PubMedCrossRefGoogle Scholar
  24. 24.
    Alzoghaibi MA, Bahammam AS (2012) The effect of one night of continuous positive airway pressure therapy on oxidative stress and antioxidant defense in hypertensive patients with severe obstructive sleep apnea. Sleep Breath 16:499–504PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Peter Celec
    • 1
    • 2
    • 3
    Email author
  • Ingrid Jurkovičová
    • 4
  • Roman Buchta
    • 1
  • Ivan Bartík
    • 1
  • Roman Gardlík
    • 1
  • Roland Pálffy
    • 1
  • Imrich Mucska
    • 4
  • Július Hodosy
    • 1
    • 4
    • 5
  1. 1.Institute of Molecular BiomedicineComenius UniversityBratislavaSlovak Republic
  2. 2.Institute of PathophysiologyComenius UniversityBratislavaSlovakia
  3. 3.Department of Molecular BiologyComenius UniversityBratislavaSlovakia
  4. 4.University HospitalBratislavaSlovakia
  5. 5.Institute of PhysiologyComenius UniversityBratislavaSlovakia

Personalised recommendations