Molecular Imaging in Synthetic Biology, and Synthetic Biology in Molecular Imaging
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Biomedical synthetic biology is an emerging field in which cells are engineered at the genetic level to carry out novel functions with relevance to biomedical and industrial applications. This approach promises new treatments, imaging tools, and diagnostics for diseases ranging from gastrointestinal inflammatory syndromes to cancer, diabetes, and neurodegeneration. As these cellular technologies undergo pre-clinical and clinical development, it is becoming essential to monitor their location and function in vivo, necessitating appropriate molecular imaging strategies, and therefore, we have created an interest group within the World Molecular Imaging Society focusing on synthetic biology and reporter gene technologies. Here, we highlight recent advances in biomedical synthetic biology, including bacterial therapy, immunotherapy, and regenerative medicine. We then discuss emerging molecular imaging approaches to facilitate in vivo applications, focusing on reporter genes for noninvasive modalities such as magnetic resonance, ultrasound, photoacoustic imaging, bioluminescence, and radionuclear imaging. Because reporter genes can be incorporated directly into engineered genetic circuits, they are particularly well suited to imaging synthetic biological constructs, and developing them provides opportunities for creative molecular and genetic engineering.
Key wordsSynthetic biology Imaging Reporter genes
We thank the members of the Shapiro and Gilad labs and the founding members of the Synthetic Biology and Reporter Genes (SyBRG) interest group of the World Molecular Imaging Society for their contributions to this field and the ideas presented in this article. In addition to the authors, founding members of SyBRG include Christopher Contag, Michal Neeman, Roger Tsien, David Piwnica-Worms, Michael Lin, Daniel Turnbull, Stuart Foster, Michael McMahon, Jeff Bulte, Brian Rutt, Vladimir Ponomarev, Erik Shapiro, Alan Jasanoff, Jeffrey Cirillo, Vasilis Ntziachristos, Jianghong Rao, Moriel Vandsburger, Gil Westmeyer, Brian Chow, and Il Minn. We also note with regret that, due to space limitations, we were not able to cite all the relevant work in this field and instead reference a smaller number of examples.
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Conflict of Interest
The authors declare that they have no conflict of interest.
- 1.Cameron DE, Bashor CJ, Collins JJ (2014) A brief history of synthetic biology. Nat Rev MicrobiolGoogle Scholar
- 4.Slomovic S, Pardee K, Collins JJ (2015) Synthetic biology devices for in vitro and in vivo diagnostics. Proceedings of the National Academy of Sciences 112:14429–14435Google Scholar
- 12.Kotula JW, Kerns SJ, Shaket LA, et al. (2014) Programmable bacteria detect and record an environmental signal in the mammalian gut. Proceedings of the National Academy of Sciences 111:4838–4843Google Scholar
- 50.Patrick PS, Rodrigues TB, Kettunen MI, Lyons SK, Neves AA, Brindle KM (2015) Development of Timd2 as a reporter gene for MRI. Magnetic resonance in medicineGoogle Scholar
- 54.Schilling F, Ros S, Hu D-E, et al. (2016) MRI measurements of reporter-mediated increases in transmembrane water exchange enable detection of a gene reporter. Nat Biotech advance online publicationGoogle Scholar
- 57.Bar-Shir A, Bulte JW, Gilad AA (2015) Molecular Engineering of Nonmetallic Biosensors for CEST MRI. ACS Chem BiolGoogle Scholar
- 70.Jiang Y, Sigmund F, Reber J, et al. (2015) Violacein as a genetically-controlled, enzymatically amplified and photobleaching-resistant chromophore for optoacoustic bacterial imaging. Scientific reports 5Google Scholar
- 74.Deckers R, Quesson B, Arsaut J, Eimer S, Couillaud F, Moonen CT (2009) Image-guided, noninvasive, spatiotemporal control of gene expression. Proceedings of the National Academy of Sciences 106:1175–1180Google Scholar