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MUC1 Aptamer-Based Near-Infrared Fluorescence Probes for Tumor Imaging

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Abstract

Purpose

DNA aptamer (APT) is able to bind to Mucin 1 (MUC1) specifically. The possibility of APT acting as a moiety to construct tumor-targeting probes was investigated.

Procedures

A near-infrared (NIR) fluorescent dye (MPA) and polyethylene glycol (PEG) were conjugated to APT to form APT-MPA and APT-PEG-MPA. The successful synthesis of the two probes was characterized via thin layer chromatography (TLC) and optical spectra. The tumor-targeting efficacy of the probes was evaluated in detail at cell level and animal level, respectively.

Results

The results indicated that MPA and PEG were successfully coupled with APT. APT-based probes were mediated by Mucin 1 into tumor cells, and PEG-modified probe exhibited higher cell affinity.

Conclusions

The aptamer-based NIR fluorescent probes are promising candidates for tumor imaging and diagnosis.

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Acknowledgments

The authors are grateful to Natural Science Foundation Committee of China (NSFC 61335007, 81371684, 81220108012, 81000666, 81171395, and 81328012), the Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University (no. SKLNMZZYQ201403), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions for their financial support.

Conflict of Interest

The authors declare that they have no conflicts of interest

Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009, China

    Haiyan Chen, Juan Zhao, Min Zhang, Yuxiang Ma & Yueqing Gu

  2. School of Pharmacy, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009, China

    Haibo Yang

Authors
  1. Haiyan Chen
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  2. Juan Zhao
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  3. Min Zhang
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  4. Haibo Yang
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  5. Yuxiang Ma
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  6. Yueqing Gu
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Corresponding author

Correspondence to Yueqing Gu.

Additional information

Haiyan Chen, Juan Zhao, and Min Zhang contributed equally to this work.

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Chen, H., Zhao, J., Zhang, M. et al. MUC1 Aptamer-Based Near-Infrared Fluorescence Probes for Tumor Imaging. Mol Imaging Biol 17, 38–48 (2015). https://doi.org/10.1007/s11307-014-0763-y

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  • DOI: https://doi.org/10.1007/s11307-014-0763-y

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