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Dynamic Monitoring of Apoptosis in Chemotherapies with Multiple Fluorescence Reporters

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Abstract

Purpose

The aim of the study is to dynamically and non-invasively monitor the temporal relationship among caspase-3, BID, and cytochrome c in chemotherapy.

Procedures

ASTC-a-1 cells expressing the corresponding fluorescence reporters were treated with Taxol or cisplatin and imaged using FRET and fluorescence overlapping technique. Western blot was performed to validate the fluorescence analysis.

Results

In fluorescence imaging analysis, Taxol-induced apoptosis showed caspase-3 activation (13 h 50 min) was prior to BID cleavage (15 h 10 min) and subsequent significant cytochrome c release (17 ~ 18 h 20 min), whereas the cisplatin-induced apoptosis showed BID cleavage (5 h 40 min) and significant cytochrome c release (7 ~ 8 h 20 min) were prior to caspase-3 activation (14 h 20 min). Western blot further validated the results above.

Conclusions

The new approach successfully reveals the difference in temporal signaling apoptosis events between Taxol and cisplatin. It may help us come to a better understanding of the detailed mechanisms in chemotherapeutic-agents-induced apoptosis.

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Acknowledgements

This research is supported in part by the National Natural Science Foundation of China (30470494; 30627003) and the Natural Science Foundation of Guangdong Province (7117865).

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Authors and Affiliations

  1. MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, 510631, China

    Yunlong Li, Da Xing & Qun Chen

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  1. Yunlong Li
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  2. Da Xing
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  3. Qun Chen
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Corresponding author

Correspondence to Da Xing.

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Li, Y., Xing, D. & Chen, Q. Dynamic Monitoring of Apoptosis in Chemotherapies with Multiple Fluorescence Reporters. Mol Imaging Biol 11, 213–222 (2009). https://doi.org/10.1007/s11307-008-0195-7

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  • DOI: https://doi.org/10.1007/s11307-008-0195-7

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