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FDG-PET/CT of Head and Neck Squamous Cell Carcinoma in a Rat Model

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Abstract

Purpose

The aim of this study was to demonstrate the ability to use human clinical positron emission tomography/computed tomography (PET/CT) to detect and investigate head and neck cancers chemically induced by 4-nitroquinoline-1-oxide (4-NQO) in a rat model.

Study design

The study design was prospective animal research.

Procedures

A head and neck squamous cell carcinoma was established in 20 immunocompetent rats, who drank a 4-NQO solution during 16 weeks. 2-Deoxy-2-[F-18]fluoro-d-glucose (FDG)-PET/CT was performed for five of them, 34 weeks after the start of the experiment to characterize the tumors. A day following the FDG-PET/CT, rats were euthanized and pathological features were evaluated by hematoxylin–eosin staining.

Results

All rats had head and neck tumor at various locations at 34 weeks. Among the five rats selected for having FDG-PET/CT, the clinical examination detected exophytic tumors grown in the oral cavity for three of them (one on the inferior lip, one on the hard palate, and one on the internal side of the cheek). FDG-PET/CT confirmed the presence of those tumors and detected ones located on the base of tongue for three of them. Tumor extensions were characterized and tumor metabolic volumes were measured. The smallest lesion detected measured 3 × 3 × 4 mm. Pathologic examination using hematoxylin–eosin staining confirmed squamous cell carcinoma.

Conclusions

This study demonstrated that FDG-PET/CT is a feasible examination to detect occult primary tumors in rat models. It is useful to follow tumor progression and evaluate therapeutics efficacy.

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Authors and Affiliations

  1. Department of E.N.T., University Hospital Center, 2 Avenue Martin Luther-King, 87000, Limoges, France

    Karine Aubry & Jean Pierre Bessède

  2. Department of Radiation Oncology, Ninth People’s Hospital, School of Medicine, Jiao Tong University, Shanghai, China

    Ziyang Shao

  3. Department of Nuclear Medicine, University Hospital Center, Limoges, France

    Jacques Monteil

  4. Department of Pathology, University Hospital Center, Limoges, France

    François Paraf

  5. Biochemistry and Molecular Biology, University Hospital Center, Limoges, France

    Michel Rigaud

Authors
  1. Karine Aubry
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  2. Ziyang Shao
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  3. Jacques Monteil
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  4. François Paraf
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  5. Jean Pierre Bessède
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  6. Michel Rigaud
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Corresponding author

Correspondence to Karine Aubry.

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Aubry, K., Shao, Z., Monteil, J. et al. FDG-PET/CT of Head and Neck Squamous Cell Carcinoma in a Rat Model. Mol Imaging Biol 11, 88–93 (2009). https://doi.org/10.1007/s11307-008-0183-y

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  • DOI: https://doi.org/10.1007/s11307-008-0183-y

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