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Quantitative Evaluation of 2-Deoxy-2[F-18]fluoro-d-glucose-Positron Emission Tomography Imaging on the Woodchuck Model of Hepatocellular Carcinoma with Histological Correlation



The Eastern woodchuck (Marmota monax) is considered as a naturally occurring animal model of hepatocellular carcinoma (HCC). The performance of 2-deoxy-2-[F-18]fluoro-d-glucose (FDG) for imaging HCC on the woodchuck using Positron emission tomography (PET) was investigated in this study.


Dynamic FDG-PET scans were performed on five woodchucks with HCC and one healthy woodchuck before removal and processing of the liver tissues for histology. The parameters of a two-tissue compartment model with dual input were estimated using weighted least squares (WLS).


Ten HCCs were confirmed histologically. Six HCCs had a tumor-to-liver standardized uptake value (SUV) ratio ≤1.15, a k 4 / k 3 ratio similar to that in hepatic tissues and were well-differentiated. Four HCCs had a tumor-to-liver SUV ratio >1.15, a lower k 4 / k 3 ratio than the hepatic tissues and were moderately differentiated.


Increased FDG uptake was observed in HCCs that were the least differentiated and correlated with a lower k 4 / k 3 ratio.

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We gratefully thank Joseph Molter, Gautam Desai, Jordan Dale, Anthony Megellini, and Maxwell Kotlarchyk for their kind support. We also acknowledge the laboratory animal contributions of Marmotech Inc. in the studies. This work was supported in part by the National Institute of Health (NIH CA095307).

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Correspondence to Zhenghong Lee.

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Salem, N., MacLennan, G.T., Kuang, Y. et al. Quantitative Evaluation of 2-Deoxy-2[F-18]fluoro-d-glucose-Positron Emission Tomography Imaging on the Woodchuck Model of Hepatocellular Carcinoma with Histological Correlation. Mol Imaging Biol 9, 135–143 (2007).

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Key words

  • Hepatocellular carcinoma
  • Woodchuck hepatitis virus
  • Kinetic modeling
  • Marmota monax