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Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling

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Abstract

Introduction

Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber. Recently, there has been an increase in the prevalence of obesity in canines; however, the information regarding diet treatments for such a condition is inadequate.

Objectives

Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.

Methods

Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD). Fecal metabolites were analyzed using gas chromatography time-of-flight mass spectrometry and a gas chromatography-flame ionization detector. Multivariate analyses were used to analyze and visualize the obtained data.

Results

A total of 52 metabolites were detected in the canine feces. In addition, HFD group feces contained a significantly low level of C12:0. The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).

Conclusion

For the first time, targeted metabolic profiling in the canine feces in response to three diets was performed. This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines. Furthermore, Dodamssal rice may possibly be used not only for canines, but also to treat obesity in other animals and humans.

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Funding

This work was carried out with the support of the Incheon National University Research in 2016 and “Cooperative Research Program for Agriculture Science and Technology Development (Project No: PJ01283406)”, Rural Development Administration, Republic of Korea.

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Author notes

  1. Ye Jin Kim and Jae Geun Kim have contributed equally to this work.

Authors and Affiliations

  1. Division of Life Sciences, Incheon National University, Yeonsu-gu, Incheon, 22012, Republic of Korea

    Ye Jin Kim, Jae Geun Kim & Jae Kwang Kim

  2. College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Wan-Kyu Lee

  3. National Institute of Animal Science, Rural Development Administration, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea

    Kyoung Min So

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  1. Ye Jin Kim
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  2. Jae Geun Kim
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Contributions

KMS and JKK designed the experiments and analyzed the data. YJK and JGK wrote the manuscript and performed the experiments. W-KL analyzed the data.

Corresponding authors

Correspondence to Kyoung Min So or Jae Kwang Kim.

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The authors declare to have no financial and non-financial conflict of interest.

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We followed all applicable international, national, and institutional guidelines for the care and use of animals. All procedures performed in the studies that involved animals were in accordance with the ethical standards of the institution in which the studies were conducted.

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Kim, Y.J., Kim, J.G., Lee, WK. et al. Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling. Metabolomics 15, 21 (2019). https://doi.org/10.1007/s11306-019-1479-4

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