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Genotypic and metabolic approaches towards the segregation of Klebsiella pneumoniae strains producing different antibiotic resistant enzymes

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Abstract

Introduction

Genotype and metabolomic variation are important for bacterial survival and adaptation to environmental changes.

Objectives

In this study, we compared the relationship among Klebsiella pneumoniae strains based on their genotypic and metabolic profiles. In addition, we also evaluated the association of the relationship with beta-lactamase production.

Methods

A total of 53 K. pneumoniae strains isolated in 2013–2014 from a tertiary teaching hospital in Malaysia were subjected to antimicrobial susceptibility testing (AST) via disk diffusion method and beta-lactamase production confirmation. The bacterial strains were also typed genotypically and metabolically via REP-PCR and 1H-NMR spectroscopy respectively. The concordance of the matrices derived based on genotypic and metabolic characterization was measured based on Spearman’s rank correlation.

Results

Spearman’s correlation rank showed that there is a weak but significant negative correlation between the genetic fingerprints and metabolic profiles of K. pneumoniae. Specifically, K. pneumoniae strains were clustered into five major clusters based on REP-PCR where most of the carbapenem resistant K. pneumoniae (CRKP) strains made up the major cluster. In contrast, metabolic patterns of the three groups (i.e. CRKP, extended spectrum beta-lactamase producing K. pneumoniae (ESBL), susceptible) of K. pneumoniae were clearly differentiated on PLS-DA score plots derived from 1H-NMR spectroscopy.

Conclusion

Overall, this study showed that metabolomic profiling using 1H-NMR spectroscopy is able to discriminate K. pneumoniae strains based on their beta-lactamase production status.

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Funding

This work was supported by Fundamental Research Grant Scheme (FRGS) from the Ministry of Education, Malaysia (Grant Number: FP023-2014A), University of Malaya Research Grant (UMRG) from University of Malaya (Grant Number: RP014B-14HTM) and Postgraduate Research Grant (PPP) from University of Malaya (Grant Number: PG028-2014B).

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Authors and Affiliations

  1. Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Ye Mun Low, Kartini Abdul Jabar, Mohd Yasim Md Yusof & Cindy Shuan Ju Teh

  2. Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, 57000, Kuala Lumpur, Malaysia

    Ivan Kok Seng Yap & Chun Wie Chong

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  1. Ye Mun Low
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  2. Ivan Kok Seng Yap
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  3. Kartini Abdul Jabar
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  4. Mohd Yasim Md Yusof
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  5. Chun Wie Chong
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  6. Cindy Shuan Ju Teh
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Correspondence to Cindy Shuan Ju Teh.

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Conflict of interest

Ye Mun Low, Ivan Kok Seng Yap, Kartini Abdul Jabar, Mohd Yasim Md Yusof, Chun Wie Chong and Cindy Shuan Ju Teh declare that they have no conflict of interest.

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Supplementary material 1 (DOC 19 KB)

11306_2017_1201_MOESM2_ESM.tif

Supplementary Fig. 1 Mean growth profiles (mean ± standard deviation) of the three groups of K. pneumoniae; CRKP, ESBL and susceptible. (TIF 143 KB)

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Low, Y.M., Yap, I.K.S., Abdul Jabar, K. et al. Genotypic and metabolic approaches towards the segregation of Klebsiella pneumoniae strains producing different antibiotic resistant enzymes. Metabolomics 13, 65 (2017). https://doi.org/10.1007/s11306-017-1201-3

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