Metabolite profiling of developing Camelina sativa seeds
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Camelina sativa is a Brassicaceae with interesting agronomic potential and is considered an alternative oilseed crop. Currently, Camelina is grown mainly for its seed, which shows a high oil content with an unusual fatty acid profile particularly rich in polyunsaturated fatty acids. Camelina seeds contain other potentially valuable compounds and their composition is now relatively well described. However, little information is available on the accumulation dynamics of these compounds during seed development.
Our aim is to describe the dynamics of metabolites accumulation during C. sativa seed development.
After purification by HPLC, the fractions were analyzed by LC–MS and NMR to characterize new compounds. The dynamic of metabolites accumulation during seed development was monitored during 15, 25 and 35 days after flowering, and metabolic profilings were performed by LC–MS and GC–MS.
This study describes for the first time two compounds (quercetin-5b-O-sinapyl-2″-O-apiosyl-3-O-rutinoside and epicatechin-7-O-glucose) that have not previously been identified in the seeds of C. sativa. We also show the accumulation kinetics of various metabolites involved in seed development. These investigations highlight a major reorganization of the metabolome with a depletion of the content of most primary metabolites and a high accumulation of most fatty acids, glucosinolates, flavonoids and sinapic acid derivatives.
This study resulted in the metabolic profile of C. sativa during seed development and enabled to identify two novel compounds in Camelina seeds.
KeywordsCamelina sativa Seed development NMR LC/MS GC/MS
This work was performed, in partnership with the SAS PIVERT, within the framework of the French Institute for Energy Transition (“Institut pour la Transition Energétique”—ITE) P.I.V.E.R.T. (www.institut-pivert.com < http://www.institut-pivert.com>) selected as an Investment for the Future (“Investissements d’Avenir”). This work was supported, as part of Investments for the Future, by the French Government under the reference ANR-001-01. The European Regional Development Fund (equipment acquired) is gratefully acknowledged.
Compliance with ethical standards
Conflict of interest
All the authors declare that they have no conflict of interest.
This article does not contain any studies with human participants performed by any of the authors.
- Berhow, M. A., Vaughn, S. F., Moser, B. R., Belenli, D., & Polat, U. (2014). Evaluating the phytochemical potential of Camelina: An emerging new crop of old world origin. In R. Jetter (Ed.), Recent Advances in Phytochemistry (pp. 142–148). Cham: Springer.Google Scholar
- Cui, C.-B., Tezuka, Y., Kikuchi, T., Nakano, H., Tamaoki, T., & Park, J.-H. (1992). Constituents of a fern, Davallia mariesii MOORE. IV. Isolation and structures of a novel norcarotane sesquiterperne glycoside, a chromone glucuronide, and two epicatechin glycosides. Chemical and Pharmaceutical Bulletin, 40, 2035–2040.CrossRefGoogle Scholar
- Fathiazad, F., Delazar, A., Amiri, R., & Sarker, S. (2006). Extraction of flavonoids and quantification of rutin from waste tobacco leaves. Iranian Journal of Pharmaceutical Research, 3, 222–227.Google Scholar
- Gräwe, W., & Strack, D. (1986). Partial purification and some properties of l-sinapoylglucose: choline cinapoyltransferase (“sinapine synthase”) from seeds of Raphanus sativus L. and Sinapis alba L. Zeitschrift für Naturforschung C, 41, 28–33.Google Scholar
- Guvenalp, Z., Kilic, N., Kazaz, C., Kaya, Y., & Demirezer, O. (2006). Chemical constituents of Galium tortumense. Turkish Journal of Chemistry, 30, 515–523.Google Scholar
- Marek, R., De Groot, A., Dommisse, R., Lemière, G., & Potacek, M. (1997). (+)#-Catechin: Benzoyl protection of OH groups and NMR study of products. Chemical Papers, 51, 107–110.Google Scholar
- Waraich, E. A., Ahmed, Z., Ahmad, R., Saifullah, M. Y. A., Naeem, M. S., & Rengel, Z. (2013). Camelina sativa, a climate proof crop, has high nutritive value and multiple-uses: A review. Australian Journal of Crops Science, 7, 1551–1559.Google Scholar