Characterization of ankylosing spondylitis and rheumatoid arthritis using 1H NMR-based metabolomics of human fecal extracts

Abstract

Introduction

The differences in fecal metabolome between ankylosing spondylitis (AS)/rheumatoid arthritis (RA) patients and healthy individuals could be the reason for an autoimmune disorder.

Objectives

The study explored the fecal metabolome difference between AS/RA patients and healthy controls to clarify human immune disturbance.

Methods

Fecal samples from 109 individuals (healthy controls 34, AS 40, and RA 35) were analyzed by 1H NMR spectroscopy. Data were analyzed with principal component analysis (PCA) and orthogonal projection to latent structure discriminant (OPLS-DA) analysis.

Results

Significant differences in the fecal metabolic profiles could distinguish AS/RA patients from healthy controls but could not distinguish between AS and RA patients. The significantly decreased metabolites in AS/RA patients were butyrate, propionate, methionine, and hypoxanthine. Significantly increased metabolites in AS/RA patients were taurine, methanol, fumarate, and tryptophan.

Conclusion

The metabolome variations in feces indicated AS and RA were two homologous diseases that could not be distinguished by 1H NMR metabolomics.

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Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (No. 81302936) and the Natural Science Foundation of Zhejiang Province (No. Q13H270006).

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Correspondence to Cheng-ping Wen.

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Research involving human rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional, national research committee and with the 1964 Helsinki Declaration and its later amendments.

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The authors declare no conflicts of interest.

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Informed consent was obtained from all individual participants included in the study.

Additional information

Tie-juan Shao and Zhi-xing He have contributed equally to this work.

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Shao, T., He, Z., Xie, Z. et al. Characterization of ankylosing spondylitis and rheumatoid arthritis using 1H NMR-based metabolomics of human fecal extracts. Metabolomics 12, 70 (2016). https://doi.org/10.1007/s11306-016-1000-2

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Keywords

  • Metabolomics
  • Faces
  • Ankylosing spondylitis
  • Rheumatoid arthritis
  • NMR
  • Autoimmune disorder