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Metabolomics

, Volume 11, Issue 4, pp 838–850 | Cite as

Comprehensive VOC profiling of an apple germplasm collection by PTR-ToF-MS

  • Brian FarnetiEmail author
  • Iuliia Khomenko
  • Luca Cappellin
  • Valentina Ting
  • Andrea Romano
  • Franco Biasioli
  • Guglielmo Costa
  • Fabrizio Costa
Original Article

Abstract

Fruit quality is generally represented by several components, among which aroma plays a fundamental role in determining the overall appreciation. To generate a comprehensive data inventory of aroma compounds in apple, a large collection represented by 190 apple accessions was characterized by a proton transfer reaction-time of flight-mass spectrometry (PTR-ToF-MS) instrument, a valid alternative to a gas chromatography-mass spectrometry (GS-MS) apparatus. The analytical performance of this instrument allowed to profile volatile organic compound (VOC) spectra of a portion of apple fruit flesh in a short time and efficient manner. Based on the VOC composition, the collection resulted grouped into six main clusters, mainly determined by ester and alcohols. These two VOC categories were also further exploited for the definition of an Alcohols/Esters index, which can be considered as a novel fruit quality descriptor useful for a further and more exhaustive characterization of several apple accessions. The distribution of these compounds and the possible further use of these information are discussed.

Keywords

Malus x domestica Borkh VOCs Esters Alcohols Aroma PTR-ToF-MS 

Notes

Acknowledgments

This work was supported by the Agroalimentare e Ricerca project (AGER Grant No. 2010–2119). Authors wish to thank Pierluigi Magnago and his team for the maintenance of the apple collection, and Marco Fontanari for his support in fruit sampling.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Compliance with Ethical Requirements

This article does not contain any studies with human or animal subjects.

Supplementary material

11306_2014_744_MOESM1_ESM.pptx (182 kb)
Supplementary Fig. 1. VOC production of two apple cultivars, ‘Golden Delicious’ and ‘Fuji’. In panel “a” it is depicted the difference in volatile production (esters, alcohols, carbonyl, and other compounds) between harvest and after two months of cold storage of intact apples for both cultivars, respectively. In panel “b” it is instead reported the same comparison described for panel “a” but performed on cut fruits. Each volatile chemical class, measured by PTR-ToF-MS, and expressed in ppbv, is reported in the legend
11306_2014_744_MOESM2_ESM.pdf (600 kb)
Supplementary Fig. 2. High resolution heat map and two-dimensional hierarchical dendrograms of VOCs patterns of 190 apple accessions assessed by PTR-ToF-MS
11306_2014_744_MOESM3_ESM.pdf (23 kb)
Supplementary Fig. 3. Pearson correlations of the PTR-ToF-MS masses detected among the 190 apple accessions
11306_2014_744_MOESM4_ESM.pdf (69 kb)
Supplementary Fig. 4. High resolution bar chart of average values, plus standard deviation, of volatile content of apple accessions belonging to the six clusters determined by Ward’s cluster analysis
11306_2014_744_MOESM5_ESM.pdf (196 kb)
Supplementary Fig. 5. VOC profile comparison of healthy and watercore affected apples of cvs. “Jolly” and “Seriana”
11306_2014_744_MOESM6_ESM.pptx (57 kb)
Supplementary Fig. 6. PCA score plot of volatile compounds assessed by PTR-ToF-MS on apple accessions measured during the first (white circle) and on the second (filled triangle) year
11306_2014_744_MOESM7_ESM.pptx (55 kb)
Supplementary Fig. 7. Correlation chart of the first two principal components (panels a, b) and of the index of absorbance difference (IAD, panel c) of 12 apple cultivars (showing a correlation lower than 90 %) assessed by PTR-ToF-MS and DA-meter during two the harvesting seasons
11306_2014_744_MOESM8_ESM.pptx (59 kb)
Supplementary Fig. 8. Correlation between the variation in ripening (ΔIAD, year 1 and 2) with the PC1 (a) and PC2 (b) values
11306_2014_744_MOESM9_ESM.pdf (222 kb)
Supplementary Fig. 9. High resolution bar chart of the values of the AE factor (total alcohol content over the total ester content) of the 190 apple accessions
11306_2014_744_MOESM10_ESM.pdf (85 kb)
Supplementary Table 1. Subdivision of 190 apple accession assessed by PTR-MS into 6 cluster. Cultivar underlined are those assessed for the years consecutively
11306_2014_744_MOESM11_ESM.pdf (162 kb)
Supplementary Table 2. Variance analysis of each detected mass (threshold of 25 ppbv) for the six apple clusters
11306_2014_744_MOESM12_ESM.pptx (42 kb)
Supplementary Table 3. Table of the percentages of volatiles statistically different (P < 0.01) between each clusters based on pairwise ANOVA analysis

References

  1. Abbott, J. A. (1999). Quality measurement of fruits and vegetables. Postharvest Biology and Technology, 15(3), 207–225.CrossRefGoogle Scholar
  2. Argenta, L., Fan, X., & Mattheis, J. (2002). Impact of watercore on gas permeance and incidence of internal disorders in ‘Fuji’ apples. Postharvest Biology and Technology, 24(2), 113–122.CrossRefGoogle Scholar
  3. Arvisenet, G., Billy, L., Poinot, P., Vigneau, E., Bertrand, D., & Prost, C. (2008). Effect of apple particle state on the release of volatile compounds in a new artificial mouth device. Journal of Agricultural and Food Chemistry, 56(9), 3245–3253.PubMedCrossRefGoogle Scholar
  4. Bader, G. D., & Hogue, C. W. (2003). An automated method for finding molecular complexes in large protein interaction networks. BMC Bioinformatics, 4, 2.PubMedCentralPubMedCrossRefGoogle Scholar
  5. Batt, P. J. (2006). Fulfilling customer needs in agribusiness supply chains. Acta Horticulturae, 699, 83–89.Google Scholar
  6. Berger, R. G. (1991). In H. Maarse (Ed.), Volatile compounds in food and beverages (pp. 283–297). New York: Marcel Dekker.Google Scholar
  7. Biasioli, F., Yeretzian, C., Märk, T. D., Dewulf, J., & Van Langenhove, H. (2011). Direct-injection mass spectrometry adds the time dimension to (B)VOC analysis. Trends in Analytical Chemistry, 30(7), 1003–1017.CrossRefGoogle Scholar
  8. Bourne, M. (2002). Food texture and viscosity: Concept and measurement (2nd ed.). San Diego: Academic Press.Google Scholar
  9. Brückner, B., & Wyllie, S. G. (2008). Fruit and vegetable flavour: recent advances and future prospects. Abington: Woodhead Publishing in Food Science, Technology and Nutrition, pp. 11–16 (ISBN: 978-1-84569-183-7).Google Scholar
  10. Cappellin, L., Biasioli, F., Granitto, P. M., Schuhfried, E., Soukoulis, C., Costa, F., et al. (2011a). On data analysis in PTR-TOF-MS: From raw spectra to data mining. Sensors and Actuators B, 155(1), 183–190.CrossRefGoogle Scholar
  11. Cappellin, L., Biasioli, F., Schuhfried, E., Soukoulis, C., Märk, T. D., & Gasperi, F. (2011b). Extending the dynamic range of proton transfer reaction time-of-flight mass spectrometers by a novel dead time correction. Rapid Communications in Mass Spectrometry: RCM, 25(1), 179–183.PubMedCrossRefGoogle Scholar
  12. Cappellin, L., Karl, T., Probst, M., Ismailova, O., Winkler, P. M., Soukoulis, C., et al. (2012a). On quantitative determination of volatile organic compound concentrations using proton transfer reaction time-of-flight mass spectrometry. Environmental Science and Technology, 46(4), 2283–2290.PubMedCrossRefGoogle Scholar
  13. Cappellin, L., Soukoulis, C., Aprea, E., Granitto, P., Dallabetta, N., Costa, F., et al. (2012b). PTR-ToF-MS and data mining methods: a new tool for fruit metabolomics. Metabolomics, 8(5), 761–770.CrossRefGoogle Scholar
  14. Cline, M. S., Smoot, M., Cerami, E., Kuchinsky, A., Landys, N., Workman, C., et al. (2007). Integration of biological networks and gene expression data using Cytoscape. Nature Protocols, 2(10), 2366–2382.PubMedCentralPubMedCrossRefGoogle Scholar
  15. Contreras, C., & Beaudry, R. (2013). Lipoxygenase-associated apple volatiles and their relationship with aroma perception during ripening. Postharvest Biology and Technology, 82, 28–38.CrossRefGoogle Scholar
  16. Cossins, E.A.(1978) Ethanol metabolism in plants. In D. D. Hook & R. M. M. Crawford (Eds.), Plant Life in Anaerobic Environments (pp. 169–202). Ann Arbor: Science Publishers.Google Scholar
  17. Costa, F., Cappellin, L., Longhi, S., Guerra, W., Magnano, P., Porro, D., et al. (2011). Assessment of apple (Malus x domestica Borkh.) fruit texture by a combined acoustic-mechanical profiling strategy. Postharvest Biology and Technology, 61(1), 21–28.CrossRefGoogle Scholar
  18. Dart, J.A., & Newman, S. M. (2005). Watercore of Apples. Primefacts 49 NSW Departmentof Primary Industries, pp. 2 (ISSN 1832-6668).Google Scholar
  19. De Jager, A., & de Putter, H. (1999). Preharvest factors and postharvest quality decline of apples. Acta Horticulturae, 485, 103–110.Google Scholar
  20. De Roos, K. B. (2003). Effect of texture and microstructure on flavour retention and release. International Dairy Journal, 13(8), 593–605.CrossRefGoogle Scholar
  21. Dewulf, J., Langenhove, H. V., & Wittmann, G. (2002). Analysis of volatile organic compounds using gas chromatography. Trends in Analytical Chemistry, 21(9–10), 637–646.CrossRefGoogle Scholar
  22. Dimick, P., & Hoskin, J. (1983). Review of apple flavour—State of the art. Critical Reviews in Food Science and Nutrition, 18(4), 387–409.PubMedCrossRefGoogle Scholar
  23. Dixon, J., & Hewett, E. W. (2000). Factors affecting apple aroma/flavour volatile concentration: A review. New Zealand Journal of Crop and Horticultural Science, 28(3), 155–173.CrossRefGoogle Scholar
  24. Dunemann, F., Ulrich, D., Malysheva-Otto, L., Weber, W. E., Longhi, S., Velascom, R., et al. (2012). Functional allelic diversity of the apple alcohol acyl-transferase gene MdAAT1 associated with fruit ester volatile contents in apple cultivars. Molecular Breeding, 29(3), 609–625.CrossRefGoogle Scholar
  25. Farneti, B., Schouten, R. E., Qian, T., Dieleman, J. A., Tijskens, L. M. M., & Woltering, E. J. (2013). Greenhouse climate control affects postharvest tomato quality. Postharvest Biology and Technology, 86, 354–361.CrossRefGoogle Scholar
  26. Fellman, J. K., Rudell, D. R., Mattinson, D. S., & Mattheis, J. P. (2003). Relationship of harvest maturity to flavor regeneration after CA storage of “Delicious” apples. Postharvest Biology and Technology, 27(1), 39–51.CrossRefGoogle Scholar
  27. Ferguson, I., Volz, R., & Woolf, A. (1999). Preharvest factors affecting physiological disorders of fruit. Postharvest Biology and Technology, 15(3), 255–262.CrossRefGoogle Scholar
  28. Fidler, J. C. (1968). The metabolism of acetaldehyde by plant tissues. Journal of Experimental Botany, 19(1), 41–51.CrossRefGoogle Scholar
  29. Fuhrmann, E., & Grosch, W. (2002). Character impact odorants of the apple cultivars Elstar and Cox Orange. Nahrung/Food, 46(3), 187–193.CrossRefGoogle Scholar
  30. Gilliver, P. J., & Nursten, H. E. (1976). The source of the acyl moiety in the biosynthesis of volatile banana esters. Journal of the Science of Food and Agriculture, 27(2), 152–158.CrossRefGoogle Scholar
  31. Giovannoni, J. J. (2001). Molecular biology of fruit maturation and ripening. Annual Review of Plant Physiology and Plant Molecular Biology, 52, 725–749.PubMedCrossRefGoogle Scholar
  32. Goff, S. A., & Klee, H. J. (2006). Plant volatile compounds: sensory cues for health and nutritional value? Science, 311(5762), 815–819.PubMedCrossRefGoogle Scholar
  33. Herremans, E., Melado-Herreros, A., Defraeye, T., Verlinden, B., Hertog, M., Verboven, P., et al. (2014). Comparison of X-ray CT and MRI of watercore disorder of different apple cultivars. Postharvest Biology and Technology, 87, 42–50.CrossRefGoogle Scholar
  34. Hewett, E. W. (2006). Progressive challenges in horticultural supply chains: Some future challenges. Acta Horticulturae, 712, 39–49.Google Scholar
  35. Holland, D., Larkov, O., Bar-Yaákov, I., Bar, E., Zax, A., & Brandeis, E. (2005). Developmental and varietal differences in volatiles ester formation and acetyl-CoA: Alcohol acetyl transferase activities in apple (Malus domestica Borkh.) fruit. Journal of Agricultural and Food Chemistry, 53(18), 7198–7203.PubMedCrossRefGoogle Scholar
  36. Jordan, A., Haidacher, S., Hanel, G., Hartungen, E., Mark, L., Seehauser, H., et al. (2009). A high resolution and high sensitivity proton-transfer-reaction time-of-flight mass spectrometer (PTR-TOF-MS). International Journal of Mass Spectrometry, 286(2–3), 122–128.CrossRefGoogle Scholar
  37. Kader, A. A. (2008). Perspective. Flavor quality of fruits and vegetables. Journal of the Science of Food and Agriculture, 88(11), 1863–1868.CrossRefGoogle Scholar
  38. Kasai, S., & Arakawa, O. (2010). Antioxidant levels in watercore tissue in ‘Fuji’ apples during storage. Postharvest Biology and Technology, 55(2), 103–107.CrossRefGoogle Scholar
  39. Klee, H. J. (2010). Improving the flavor of fresh fruits: Genomics, biochemistry, and biotechnology. New Phytopatologist, 187(1), 44–56.CrossRefGoogle Scholar
  40. Klee, H. J., & Giovannoni, J. J. (2011). Genetics and control of tomato fruit ripening and quality attributes. Annual Review of Genetics, 45, 41–59.PubMedCrossRefGoogle Scholar
  41. Lauri, P. É., Combe, F., & Brun, L. (2014). Regular bearing in the apple—Architectural basis for an early diagnosis on the young tree. Scientia Horticulturae, 174(22), 10–16.CrossRefGoogle Scholar
  42. Lindinger, W., Hansel, A., & Jordan, A. (1998). On-line monitoring of volatile organic compounds at pptv levels by means of proton- transfer-reaction mass spectrometry (PTR-MS)—Medical applications, food control and environmental research. International Journal of Mass Spectrometry and Ion Physics, 173(3), 191–241.CrossRefGoogle Scholar
  43. Melado-Herreros, A., Mu˜noz-García, M. A., Blanco, A., Val, J., Fernandez-Valle, M. E., & Barreiro, P. (2013). Assessment of watercore development in apples with MRI: Effect of fruit location in the canopy. Postharvest Biology and Technology, 86, 125–133.CrossRefGoogle Scholar
  44. Newcomb, R. D., Crowhurst, R. N., Gleave, A. P., Rikkerink, E. H. A., Allan, A. C., Beuning, L. L., et al. (2006). Analyses of expressed sequence tags from apple. Plant Physiology, 141(1), 147–166.PubMedCentralPubMedCrossRefGoogle Scholar
  45. Nijssen, L. M., van Ingen-Visscher, C. A., & Donders, J. J. H. (2011) VCF Volatile Compounds in Food: Database (Version 13.1). Zeist (The Netherlands).Google Scholar
  46. Nyasordzi, J., Friedman, H., Schmilovitch, Z., Ignat, T., Weksler, A., Rot, I., et al. (2013). Utilizing the IAD index to determine internal quality attributes of apples at harvest and after storage. Postharvest Biology and Technology, 77, 80–86.CrossRefGoogle Scholar
  47. Pesis, E. (2005). The role of anaerobic metabolites, acetaldehyde and ethanol, in fruit ripening, enhancement of fruit quality and fruit deterioration. Postharvest Biology and Technology, 37(1), 1–19.CrossRefGoogle Scholar
  48. Rowan, D. D., Allen, J. M., Fielder, S., & Hunt, M. B. (1999). Biosynthesis of straight-chain ester volatiles in red delicious and Granny Smith apples using deuterium-labeled precursors. Journal of Agricultural and Food Chemistry, 47(7), 2553–2562.PubMedCrossRefGoogle Scholar
  49. Rowan, D. D., Lane, H. P., Allen, J. M., Fielder, S., & Hunt, M. B. (1996). Biosynthesis of 2-methylbutyl, 2-methyl-2-butenyl, and 2-methylbutanoate esters in Red Delicious and Granny Smith apples using deuterium-labelled substrates. Journal of Agricultural and Food Chemistry, 44(10), 3276–3285.CrossRefGoogle Scholar
  50. Schaffer, R. J., Friel, E. N., Souleyre, E. J. F., Bolitho, K., Thodey, K., Ledger, S., et al. (2007). A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiology, 144(4), 1899–1912.PubMedCentralPubMedCrossRefGoogle Scholar
  51. Soukoulis, C., Cappellin, L., Aprea, E., Costa, F., Viola, R., Märk, T. D., et al. (2013). PTR-ToF-MS, a novel, rapid, high sensitivity and non-invasive tool to monitor volatile compound release during fruit post-harvest storage: the case study of apple ripening. Food Bioprocess Technology, 6(10), 2831–2843.CrossRefGoogle Scholar
  52. Ting, J. L. V., Soukoulis, C., Silcock, P., Cappellin, L., Romano, A., Aprea, E., et al. (2012). In Vitro and In Vivo flavor release from intact and fresh-cut apple in relation with genetic, textural, and physicochemical parameters. Journal of Food Science, 77(11), 1226–1233.CrossRefGoogle Scholar
  53. Ulrich, D., & Dunemann, F. (2012). Towards the development of molecular markers for apple volatiles. Flavour and Fragrance Journal, 27(4), 286–289.CrossRefGoogle Scholar
  54. Ulrich, D., Hoberg, E., & Fischer, C. (2009). Diversity and dynamic of sensory related traits in different apple cultivars. Journal of Apply Botany and Food Quality, 83, 70–75.Google Scholar
  55. Zhu, Y., Rudell, D. R., & Mattheis, J. P. (2008). Characterization of cultivar differences in alcohol acyltransferase and 1-aminocyclopropane-1-carboxylate synthase gene expression and volatile ester emission during apple fruit maturation and ripening. Postharvest Biology and Technology, 49(3), 330–339.CrossRefGoogle Scholar
  56. Ziosi, V., Noferini, M., Fiori, G., Tadiello, A., Trainotti, L., Casadoro, G., et al. (2008). A new index based on vis spectroscopy to characterize the progression of ripening in peach fruit. Postharvest Biology and Technology, 49(3), 319–329.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Brian Farneti
    • 1
    Email author
  • Iuliia Khomenko
    • 2
  • Luca Cappellin
    • 2
  • Valentina Ting
    • 2
  • Andrea Romano
    • 2
  • Franco Biasioli
    • 2
  • Guglielmo Costa
    • 1
  • Fabrizio Costa
    • 2
  1. 1.Department of Agricultural SciencesBologna UniversityBolognaItaly
  2. 2.Research and Innovation CentreFondazione Edmund MachTrentoItaly

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