Abstract
Fourier transform infrared spectroscopy was used to characterise highland and lowland populations of Polygonum minus Huds. grown in different controlled environments. A thermal perturbation technique of two-dimensional correlation infrared spectroscopy (2D-IR) correlation spectra was applied to establish differences between the populations. The absorption peaks at 3,480 cm−1 (hydroxyl group), 2,927 cm−1 (methyl group), 1,623 cm−1 (carbonyl group), and 1,068 cm−1 (C–O group) were particularly powerful in separating the populations. These peaks, which indicate the presence of carbohydrate, terpenes, amide and flavonoids were more intense for the highland populations than lowland populations, and increased in environments with a higher temperature. Wavenumbers (1,634, 669 cm−1) and (1,634, 1,555 cm−1) in the 2D-IR correlation spectra provided fingerprint signals to differentiate plants grown at different temperatures. This study demonstrates that IR fingerprinting, which combines mid-IR spectra and 2D-IR correlation spectra, can directly discriminate different populations of P. minus and the effects of temperature.
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Acknowledgments
This research is supported by the Genomics and Molecular Biology Initiative of the Malaysia Genome Institute, Ministry of Science, Technology and Innovation (07-05-MGI-GMB 004), Research University Grant under the Arus Perdana (UKM-AP-BPB-14-2009) and Fundamental Research Grant Scheme (UKM-RB-06-FRGS0102-2009). The authors would like to thank the reviewers and Prof. Dr. Michael Burrell from University of Sheffield for their constructive comments throughout the preparation of this manuscript.
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Khairudin, K., Sukiran, N.A., Goh, HH. et al. Direct discrimination of different plant populations and study on temperature effects by Fourier transform infrared spectroscopy. Metabolomics 10, 203–211 (2014). https://doi.org/10.1007/s11306-013-0570-5
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DOI: https://doi.org/10.1007/s11306-013-0570-5