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Metabonomic studies on potential plasma biomarkers in rats exposed to ionizing radiation and the protective effects of Hong Shan Capsule

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Abstract

An ultra performance liquid chromatography coupled to mass spectrometry-based metabonomic approach, combined with pattern recognition methods including PCA, PLS-DA, RF and heatmap, has been developed to characterize the global serum metabolic profile associated with ionizing radiation (IR). As the VIP-value threshold cutoff of the metabolites was set to 2, metabolites above this threshold were filtered out as potential target biomarkers. Nineteen distinct potential biomarkers in rat plasma were identified. To further elucidate the pathophysiology of IR, related metabolic pathways have been studied. It was found that IR was closely related to disturbed fatty acid metabolism, taurine and hypotaurine metabolism, sphingolipid metabolism, purine metabolism, pyrimidine metabolism, phospholipid catabolism, tryptophan metabolism, phenylalanine metabolism, and bile acid metabolism. With the presented metabonomic method, we systematically analyzed the protective effects of Traditional Chinese Medicine Hong Shan Capsule (HSC). The results demonstrated that HSC administration could provide satisfactory effects on IR through partially regulating the perturbed metabolic pathways.

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Acknowledgments

The authors appreciate the financial support from the National Major Projects of China (No. 2010ZXG0900X-005).

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Authors and Affiliations

  1. School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai, 200433, People’s Republic of China

    Yue Liu, Ze-Bin Lin, Guang-Guo Tan, Zi-Yang Lou, Jun-Ping Zhang, Zhan-Ying Hong & Yi-Feng Chai

  2. The Naval Medical Research Institute, Shanghai, 200433, People’s Republic of China

    Zhi-Yong Chu

  3. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, 200433, People’s Republic of China

    Yue Liu, Guang-Guo Tan, Zhan-Ying Hong & Yi-Feng Chai

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  1. Yue Liu
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  2. Ze-Bin Lin
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Liu, Y., Lin, ZB., Tan, GG. et al. Metabonomic studies on potential plasma biomarkers in rats exposed to ionizing radiation and the protective effects of Hong Shan Capsule. Metabolomics 9, 1082–1095 (2013). https://doi.org/10.1007/s11306-013-0529-6

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