Metabolomic and elemental profiling of melon fruit quality as affected by genotype and environment
- 1.7k Downloads
Melon (Cucumis melo L.) is a global crop in terms of economic importance and nutritional quality. The aim of this study was to explore the variability in metabolite and elemental composition of several commercial varieties of melon in various environmental conditions. Volatile and non-volatile metabolites as well as mineral elements were profiled in the flesh of mature fruit, employing a range of complementary analytical technologies. More than 1,000 metabolite signatures and 19 mineral elements were determined. Data analyses revealed variations related to factors such as variety, growing season, contrasting agricultural management practices (greenhouse vs. field with or without fruit thinning) and planting date. Two hundred and ninety-one analytes discriminated two contrasting varieties, one from the var. inodorous group and the other from the var. cantaloupensis group. Two hundred and eighty analytes discriminated a short shelf-life from a mid-shelf-life variety within the var. cantaloupensis group. Three hundred and twenty-seven analytes discriminated two seasons, and two hundred and fifty-two analytes discriminated two contrasting agricultural management practices. The affected compound families greatly depended on the factor studied. The compositional variability of identified or partially identified compounds was used to study metabolite and mineral element co-regulation using correlation networks. The results confirm that metabolome and mineral element profiling are useful diagnostic tools to characterize the quality of fruits cultivated under commercial conditions. They can also provide knowledge on fruit metabolism and the mechanisms of plant response to environmental modifications, thereby paving the way for metabolomics-guided improvement of cultural practices for better fruit quality.
KeywordsCucumis melo Fruit quality 1H-NMR MS Metabolomics Mineral elements
- Carrari, F., Baxter, C., Usadel, B., et al. (2006). Integrated analysis of metabolite and transcript levels reveals the metabolic shifts that underlie tomato fruit development and highlight regulatory aspects of metabolic network behavior. Plant Physiology, 142, 1380–1396.PubMedCrossRefGoogle Scholar
- De Vos, R. C. H., Hall, R., & Moing, A. (2011). Metabolomics of a model fruit: tomato. In R. Hall (Ed.), Biology of plant metabolomics (pp. 109–155). Oxford: Wiley-Blackwell Ltd.Google Scholar
- Dufault, R. J., Korkmaz, A., Ward, B. K., & Hassell, R. L. (2006). Planting date and cultivar affect melon quality and productivity. HortScience, 41, 1559–1564.Google Scholar
- Fan, T. W. M. (1996). Metabolite profiling by one- and two-dimensional NMR analysis of complex mixtures. Progress in Nuclear Magnetic Resonance Spectroscopy, 28, 161–219.Google Scholar
- Fish, W. W. & Bruton, B. D. (2010). Quantification of l-citrulline and other physiologic amino acids in watermelon and selected cucurbits. In Cucurbitacae 2010, Charleston, SC (pp. 152–154).Google Scholar
- Gibon, Y., Rolin, D., Deborde, C., Bernillon, S. & Moing, A. (2012). New opportunities in metabolomics and biochemical phenotyping for plant systems biology. In U. Roessner (Ed.), Metabolomics: InTech. http://www.intechopen.com/articles/show/title/new-opportunities-in-metabolomics-and-biochemical-phenotyping-for-plant-systems-biology.
- Hansen, T. H., Laursen, K. H., Persson, D. P., et al. (2009). Micro-scaled high-throughput digestion of plant tissue samples for multi-elemental analysis. Plant Methods, 5. doi: 10.1186/1746-4811-1185-1112.
- Lester, G. E. (2005). Whole plant applied potassium: effects on cantaloupe fruit sugar content and related human wellness compounds. In Proceedings of the fifth international postharvest symposium (pp. 487–492) Verona, Italy, 6–11 June, 2004. Leuven: International Society for Horticultural Science (ISHS).Google Scholar
- Lester, G. (2006). Consumer preference quality attributes of melon fruits. In Proceedings of the IVth international conference on managing quality in chains MQUIC 2006: Integrated view on fruits and vegetables quality (Vol. 1, pp. 175–181), Bangkok, Thailand, 7–10 August 2006.Google Scholar
- Lester, G. E., & Crosby, K. M. (2002). Ascorbic acid, folic acid, and potassium content in postharvest green-flesh honeydew muskmelons: influence of cultivar, fruit size, soil type, and year. Journal of the American Society for Horticultural Science, 127, 843–847.Google Scholar
- Lester, G. E., & Grusak, M. A. (1999). Postharvest application of calcium and magnesium to honeydew and netted muskmelons: effects on tissue ion concentrations, quality, and senescence. Journal of the American Society for Horticultural Science, 124, 545–552.Google Scholar
- Poiroux-Gonord, F., Bidel, L. P. R., Fanciullino, A. L., et al. (2010). Health benefits of vitamins and secondary metabolites of fruits and vegetables and prospects to increase their concentrations by agronomic approaches. Journal of Agricultural and Food Chemistry, 58, 12065–12082.CrossRefGoogle Scholar
- Shannon, P., Markiel, A., Ozier, O., et al. 2002. Cytoscape: A software environment for integrated models of biomolecular interaction networks. In Proceedings of the 3rd international conference on systems biology, ICSB 2002 (pp. 2498–2504), Stockholm, Sweden.Google Scholar
- Stewart, D., Sheperd, L. V. T., Hall, R. D., & Fraser, P. D. (2011). Crops and tasty, nutritious food: How can metabolomics help? In R. D. Hall (Ed.), Biology of plant metabolomics (pp. 181–217). Chichester: Wiley-Blackwell.Google Scholar
- Taureilles-Saurel, C., Romieu, C. G., Robin, J.-P., & Flanzy, C. (1995). Grape (Vitis vinifera L.) malate dehydrogenase. II. Characterization of the major mitochondrial and cytosolic isoforms and their role in ripening. American Journal of Enology and Viticulture, 46, 29–36.Google Scholar
- Tikunov, Y., Laptenok, S., Hall, R., Bovy, A., & de Vos, R. (2012). MSClust: A tool for unsupervised mass spectra extraction of chromatography–mass spectrometry ion-wise aligned data. Metabolomics. doi: 10.1007/s11306-011-0368-2.
- Verhoeven, H. A., Jonker, H., de Vos, R. C. H., & Hall, R. D. (2012). Solid-phase micro-extraction (SPME) GC-MS analysis of natural volatile components in melon and rice. In N. G. Hardy & R. D. Hall (Eds.), Plant metabolomics methods. Ithaca: Humana Press.Google Scholar
- Wada, M. (1930). Über citrullin, eine neue aminosäure im presssaft der wassermelone. Citrullus vulgaris Schrad. Biochemische Zeitschrift, 224, 420.Google Scholar