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Metabolomic screening and star pattern recognition by urinary amino acid profile analysis from bladder cancer patients

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Abstract

Metabolomic analysis of urinary amino acids (AAs) from patients with bladder cancer was performed by gas chromatography–mass spectrometry. The β-aminoisobutyric acid (P < 0.05) and pyroglutamic acid (P < 0.03) levels among 21 AAs had relatively low P-values in the cancer group. The distorted star pattern of the cancer group was different from the heneicosagonal shape of the control mean. The present metabolomic screening combined with star pattern recognition method as clinical monitoring tool might be useful for the visual discrimination of bladder cancer group from normal group.

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Fig. 1

Abbreviations

AAs:

Amino acids

GC:

Gas chromatography

MS:

Mass spectrometry

SIM:

Selected ion monitoring

EOC/TBDMS:

Ethoxycarbonyl/tert-butyldimethylsilyl

AD:

Alzheimer’s disease

IS:

Internal standard

ECF:

Ethyl chloroformate

MTBSTFA:

N-Methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide

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Acknowledgements

This work was supported by the ERC program of MOST/KOSEF (grant # R11-2000-075-01004-0 (2007)) and National Research Foundation of Korea Grant funded by the Korean Government (KRF-2008-314-E00183). This research was performed with an Agilent 6890 N GC interfaced to an Agilent 5975B MSD in Cooperative Center for Research Facilities of Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.

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Correspondence to Man-Jeong Paik.

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J.-W. Kim and G. Lee contributed equally to this work.

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Kim, JW., Lee, G., Moon, SM. et al. Metabolomic screening and star pattern recognition by urinary amino acid profile analysis from bladder cancer patients. Metabolomics 6, 202–206 (2010). https://doi.org/10.1007/s11306-010-0199-6

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