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Principal component and hierarchical clustering analysis of metabolites in destructive weeds; polygonaceous plants

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Abstract

Comprehensive analysis of metabolites using capillary electrophoresis–mass spectrometry was carried out in harmful weeds belonging to Polygonaceae. A principal component analysis revealed clear distinctions among eight Rumex species and Fallopia japonica. Hierarchical clustering data showed that respective metabolites can be grouped due to species differences. There was a positive relationship between oxalate and citrate, oxalate and ascorbate, and oxalate and glutamine. The amount of oxalate per leaf fresh weight was not affected by increased concentrations of exogenously supplied nutrients from Hoagland’s formulation in one of the most destructive weeds R. obtusifolius. The oxalate accumulation in this plant is independent of external nutrient level, where nutrient-rich environments apparently stimulate internal constituents such as amino acids and other metabolites.

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References

  • Beerling, D. J., Bailey, J. P., & Conolly, A. P. (1994). Fallopia japonica (Houtt.) Ronse Decraene (Reynoutria japonica Houtt.; Polygonum cuspidatum Sieb. & Zucc.). Journal of Ecology, 82, 959–979.

    Article  Google Scholar 

  • Davoine, C., Le Deunff, E., Ledger, N., Avice, J. C., Billard, J. P., Dumas, B., et al. (2001). Specific and constitutive expression of oxalate oxidase during the ageing of leaf sheaths of ryegrass stubble. Plant, Cell and Environment, 24, 1033–1043.

    Article  CAS  Google Scholar 

  • Dickie, C. W., Hamann, M. H., Carroll, W. D., & Chow, F. (1978). Oxalate (Rumex venosus) poisoning in cattle. Journal of the American Veterinary Medical Association, 173, 73–74.

    CAS  PubMed  Google Scholar 

  • Foyer, C. H., Parry, M., & Noctor, G. (2003). Markers and signals associated with nitrogen assimilation in higher plants. Journal of Experimental Botany, 54, 585–593.

    Article  CAS  PubMed  Google Scholar 

  • Frye, A. S. L., & Kron, K. A. (2003). rbcL phylogeny and character evolution in Polygonaceae. Systematic Botany, 28, 326–332.

    Google Scholar 

  • Hashida, S. N., Takahashi, H., Kawai-Yamada, M., & Uchimiya, H. (2007). Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltrasferase (AtNMNAT) is required for pollen tube growth. Plant Journal, 49, 694–703.

    Article  CAS  PubMed  Google Scholar 

  • Holm, L. G., Plucknett, D. L., Pancho, J. V., & Herberger, J. P. (1977). Rumex crispus and Rumex obtusifolius. In L. G. Holm (Ed.), The world’s worst weeds: Distribution and biology (pp. 401–408). Honolulu: University Press of Hawaii.

    Google Scholar 

  • Le Deunff, E., Davoine, C., Le Dantec, C., Billard, J. P., & Huault, C. (2004). Oxidative burst and expression of germin/oxo genes during wounding of ryegrass leaf blades: Comparison with senescence of leaf sheaths. Plant Journal, 38, 421–431.

    Article  CAS  PubMed  Google Scholar 

  • Nashiki, M., Sawada, H., Harashima, N., & Sato, K. (1988). Studies on weed management in pastures. III. Decrease in the population density of orchardgrass in a sward infested with broadleaf-dock. Weed Research, Japan, 33, 253–259.

    Google Scholar 

  • Noctor, G., & Foyer, C. H. (1998). Ascorbate and Glutathione: Keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 249–279.

    Article  CAS  PubMed  Google Scholar 

  • Novitskaya, L., Trevanion, S., Driscoll, S. D., Foyer, C. H., & Noctor, G. (2002). How does photorespiration modulate leaf amino acid contents? A dual approach through modeling and metabolite analysis. Plant, Cell and Environment, 25, 821–836.

    Article  CAS  Google Scholar 

  • Okazaki, K., Oka, N., Shinano, T., Osaki, M., & Takebe, M. (2008). Differences in the metabolite profiles of spinach (Spinacia oleracea L.) leaf in different concentrations of nitrate in the culture solution. Plant and Cell Physiology, 49, 170–177.

    Article  CAS  PubMed  Google Scholar 

  • Panciera, R. J., Martin, T., Burrows, G. E., Taylor, D. S., & Rice, L. E. (1990). Acute oxalate poisoning attributable to ingestion of curly dock (Rumex crispus) in sheep. Journal of the American Veterinary Medical Association, 196, 1981–1984.

    CAS  PubMed  Google Scholar 

  • Reig, R., Sanz, P., Blanche, C., Fontarnau, R., Dominguez, A., & Corbella, J. (1990). Fatal poisoning by Rumex crispus (curled dock): Pathological findings and application of scanning electron microscopy. Veterinary and Human Toxicology, 32, 468–470.

    CAS  PubMed  Google Scholar 

  • Sato, S., Soga, T., Nishioka, T., & Tomita, M. (2004). Simultaneous determination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection. Plant Journal, 40, 151–163.

    Article  CAS  PubMed  Google Scholar 

  • Siener, R., Hönow, R., Seidler, A., Voss, S., & Hesse, A. (2006). Oxalate contents of species of the Polygonaceae, Amaranthaceae and Chenopodiaceae families. Food Chemistry, 98, 220–224.

    Article  CAS  Google Scholar 

  • Spoerke, D. G., & Smolinke, S. C. (1990). Oxalates. In D. G. Spoerke & S. C. Smolinke (Eds.), Toxicity of houseplants (pp. 29–32). Florida: CRC press.

    Google Scholar 

  • Takahashi, H., Hayashi, M., Goto, F., Sato, S., Soga, T., Nishioka, T., et al. (2006a). Evaluation of metabolic alteration in transgenic rice overexpressing dihydrofavonol-4-reductase. Annals of Botany, 98, 819–825.

    Article  CAS  PubMed  Google Scholar 

  • Takahashi, H., Watanabe, A., Tanaka, A., Hashida, S., Kawai-Yamada, M., Sonoike, K., et al. (2006b). Chloroplast NAD kinase is essential for energy transduction though the xanthophylls cycle in photosynthesis. Plant and Cell Physiology, 47, 1678–1682.

    Article  CAS  PubMed  Google Scholar 

  • Tian, H., Jiang, L., Liu, E., Zhang, J., Liu, F., & Peng, X. (2008). Dependence of nitrate-induced oxalate accumulation on nitrate reduction in rice leaves. Physiologia Plantarum, 133, 180–189.

    Article  CAS  PubMed  Google Scholar 

  • Zaller, J. G. (2006). Sheep grazing vs cutting: Regeneration and soil nutrient exploitation of the grassland weed Rumex obtusifolius. BioControl, 51, 837–850.

    Article  Google Scholar 

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Acknowledgements

We thank to Dr. Masaki Tateno, Dr. Satoshi Kobayashi, Dr. Mitsunori Hayashi, Dr. Takuji Nakamura and Dr. Jun Ishii for providing materials and helpful advice. This research was supported by a grant from the MEXT, Japan, CREST, JST, Japan, and the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).

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Authors and Affiliations

  1. Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan

    Atsuko Miyagi, Kentaro Takahara, Takayuki Hirabayashi, Yoshiki Nishimura, Takafumi Tezuka, Maki Kawai-Yamada & Hirofumi Uchimiya

  2. Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan

    Hideyuki Takahashi & Hirofumi Uchimiya

  3. Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Yoshiki Nishimura

  4. School of Health and Human Life, Nagoya Bunri University, Inazawa, 492-8520, Japan

    Takafumi Tezuka

  5. Department of Environmental Science and Human Engineering, Saitama University, 225 Shimo-Okubo, Sakura-Ku, Saitama, 338-0825, Japan

    Maki Kawai-Yamada

  6. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 4-1-8, Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Maki Kawai-Yamada

Authors
  1. Atsuko Miyagi
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  2. Hideyuki Takahashi
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  3. Kentaro Takahara
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  4. Takayuki Hirabayashi
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  5. Yoshiki Nishimura
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  6. Takafumi Tezuka
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  7. Maki Kawai-Yamada
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  8. Hirofumi Uchimiya
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Correspondence to Hirofumi Uchimiya.

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Miyagi, A., Takahashi, H., Takahara, K. et al. Principal component and hierarchical clustering analysis of metabolites in destructive weeds; polygonaceous plants. Metabolomics 6, 146–155 (2010). https://doi.org/10.1007/s11306-009-0186-y

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  • DOI: https://doi.org/10.1007/s11306-009-0186-y

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