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Differential metabolic response of narrow leafed lupine (Lupinus angustifolius) leaves to infection with Colletotrichum lupini

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Abstract

Flavones and isoflavones are a major group of phenolic secondary metabolites which occur in leaves of narrow leafed lupine (Lupinus angustifolius) either as free aglycones or in a form of glycosides and malonyl-glycosides. Profiles of phenolic compounds in leaves of seedlings infected with anthracnose causing fungus Colletotrichum lupini were compared to those of healthy plants. A HPLC with diode array UV detector was used as the analytical method and identification of these secondary metabolites was confirmed with a HPLC/MSn instrument. Isomers of several target compounds differing in the glycosilation and/or malonylation pattern were detected in the studied samples. However, the application of standard HPLC with C18 columns resulted in the co-elution of several glyconjugates in single chromatographic peaks whereas for isoflavonoid aglycones complete resolution was achieved. Lupine plants grown in a greenhouse were either sprayed with the C. lupini spore suspension or the suspension was spotted on to wounded leaves. Profiles of the isoflavones were altered in result to infection with both methods. In particular, the concentration of isoflavone free aglycones detected in extracts from diseased plants was substantially increased in all of the studied samples. However, the pattern of these compounds depended on the age of lupine leaves as well as on the method of infection. Synthesis of luteone and 2′-hydroxygenistein was enhanced in the youngest leaves of plants sprayed with spores as well as in wound-infected leaves. Wighteone synthesis was induced mainly in older leaves of plants sprayed with the spore suspension.

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Acknowledgment

This work was supported by the Polish Ministry of Science (grant No 2 P06A 030 29). We are grateful to Mrs. E. Lewartowska (IPG PAS) for maintaining the fungal culture and her skilful help in infection of lupine plants.

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry PAS, Noskowskiego 12/14, 61-704, Poznań, Poland

    Dorota Muth & Maciej Stobiecki

  2. Institute of Plant Genetics PAS, Strzeszyńska 34, 60-479, Poznań, Poland

    Piotr Kachlicki, Paweł Krajewski & Marcin Przystalski

Authors
  1. Dorota Muth
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  2. Piotr Kachlicki
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  3. Paweł Krajewski
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  4. Marcin Przystalski
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  5. Maciej Stobiecki
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Corresponding author

Correspondence to Maciej Stobiecki.

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Figure 1s

MSn mass spectra of 12 flavonoid compounds identified in leaves of Lupinus angustifolius plants registered in positive and negative ion modes (PDF 219 kb)

Figure 2s

Average amounts of free isoflavone aglycones measured in leaves of control plants and plants sprayed with Colletotrichum lupini spore suspension (PDF 90 kb)

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Muth, D., Kachlicki, P., Krajewski, P. et al. Differential metabolic response of narrow leafed lupine (Lupinus angustifolius) leaves to infection with Colletotrichum lupini . Metabolomics 5, 354–362 (2009). https://doi.org/10.1007/s11306-009-0162-6

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  • DOI: https://doi.org/10.1007/s11306-009-0162-6

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