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Purinergic system ecto-enzymes participate in the thromboregulation of patients with indeterminate form of Chagas disease

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Abstract

Chagas disease (CD) is a chronic and endemic illness caused by the parasite Trypanosoma cruzi. Microvascular disturbances play an important role in the progress of the disease. The purinergic signaling system participates in regulatory functions, such as immunomodulation, neuroprotection, and thromboregulation. This study aimed to investigate the activities of purinergic system ecto-enzymes present on the platelet surface and the platelet aggregation profile from patients with indeterminate form of Chagas disease (IFCD). Thirty patients diagnosed with IFCD and 30 healthy subjects were selected. Ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-nucleotide pyrophosphatase/phosphodiesterase (E-NPP), ecto-5′-nucleotidase (E-5′-NT) and ecto-adenosine deaminase (E-ADA) activities were measured in platelets isolated from these individuals as well as the platelet aggregation. Results demonstrated an increase of 21 % in the E-NPP activity and 30 % in the E-5′-NT activity in IFCD group (P < 0.05); however, a decrease of 34 % in the E-ADA activity was determined in the same group (P < 0.001). A significant decrease of 12.7 % and 12.8 % in the platelet aggregation of IFCD group in two different concentrations of ADP (5 and 10 μM) was observed, respectively (P < 0.05). Increased E-NPP and E-5-NT activities as well as decreased E-ADA activity in platelets of patients with IFCD contributed to decrease platelet aggregation, suggesting that the purinergic system is involved in the thromboregulation process in these patients, since adenosine (the final product of ATP hydrolysis) has cardioprotective and vasodilator effects that prevent the clinical progress of the disease.

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Acknowledgements

This study was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundo de Incentivo a Pesquisa (FIPE/UFSM), Brazil.

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Author declares there are no actual or potential conflicts of interest.

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Authors and Affiliations

  1. Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Viviane do Carmo Gonçalves Souza, Karine B. Schlemmer, Cristiano B. Noal, Jeandre A. S. Jaques, Luiz Felipe D. Carli & Daniela B. R. Leal

  2. Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Maria B. Moretto

  3. Departamento de Química, Laboratório de Enzimologia Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Margarete D. Bagatini, Victor C. Pimentel, Cláudio A. M. Leal & Maria R. C. Schetinger

  4. Laboratório de Análises Clínicas, Hospital Universitário de Santa Maria, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Juliana Fleck

  5. Departamento de Microbiologia e Parasitologia/CCS/UFSM, Universidade Federal de Santa Maria (UFSM), Prédio 20—Sala 4102, Santa Maria, Brazil

    Daniela B. R. Leal

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  1. Viviane do Carmo Gonçalves Souza
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  2. Karine B. Schlemmer
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  3. Cristiano B. Noal
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  4. Jeandre A. S. Jaques
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  5. Margarete D. Bagatini
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  6. Victor C. Pimentel
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  7. Luiz Felipe D. Carli
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  8. Cláudio A. M. Leal
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  9. Juliana Fleck
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  10. Maria B. Moretto
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  11. Maria R. C. Schetinger
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  12. Daniela B. R. Leal
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Correspondence to Daniela B. R. Leal.

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Souza, V.d.C.G., Schlemmer, K.B., Noal, C.B. et al. Purinergic system ecto-enzymes participate in the thromboregulation of patients with indeterminate form of Chagas disease. Purinergic Signalling 8, 753–762 (2012). https://doi.org/10.1007/s11302-012-9322-7

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