Abstract
Pomegranate (Punica granatum L.) germplasm collections are increasingly appreciated as a repository for the genetic improvement of species, and their genetic analysis is an essential prerequisite for their utilization in pomegranate breeding. Sufficient markers are a prerequisite for genetic analysis. In this study, we identified 36,792 simple sequence repeat (SSR) markers in the pomegranate genome. Dinucleotide repeats are the most abundant SSRs, and of the mono-, di-, tri-, and tetranucleotide repeats, A/T, AT/AT, AAT/ATT, and AAAT/ATTT were the most dominant motif type. There were only 1.80% SSR motifs located in the coding regions, while most (98.20%) SSR motifs were located in noncoding regions. We confirmed the reliability of 50 SSR markers by PCR amplification, from which 11 highly polymorphic SSR markers were selected to study the genetic diversity of 218 pomegranate accessions. A total of 63 alleles was detected for the 11 SSR primers, ranging from 2 (PG152) to 9 (PG093, PG080) across the pomegranate accessions. The polymorphism information content values of each SSR primer pair ranged from 0.22 to 0.70, with a mean value of 0.45. Based on population structure analysis, these accessions were clustered into three main populations and 42 accessions were chosen as the core set. The availability of these SSR markers and the clustering result for the pomegranate germplasm provide new important information for pomegranate genetics and breeding programs.
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Acknowledgments
We are thankful for Professor Pan Yongbao (Agricultural Research Services, United States Department of Agriculture) for the help in polishing the language.
Funding
This research was funded by the Project of the Key Laboratory of Anhui Academy of Agricultural Sciences (2019YL013) and the Natural Science Foundation of Anhui Province (1708085MC85).
Data archiving statementThis research contains no data that requires submission to a public database. The 36,792 SSR motifs found in this study are listed in Supplementary Table S3.
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Communicated by G. G. Vendramin
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Liu, C., Li, J. & Qin, G. Genome-wide distribution of simple sequence repeats in pomegranate and their application to the analysis of genetic diversity. Tree Genetics & Genomes 16, 36 (2020). https://doi.org/10.1007/s11295-020-1428-4
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DOI: https://doi.org/10.1007/s11295-020-1428-4