Tree Genetics & Genomes

, 14:80 | Cite as

Genome-wide characterization of protein phosphatase 2C genes in Populus euphratica and their expression profiling under multiple abiotic stresses

  • Jianbo Li
  • Huixia Jia
  • Jin Zhang
  • Jingsuan Sun
  • Yongan Zhang
  • Mengzhu Lu
  • Xuebing XinEmail author
  • Jianjun HuEmail author
Original Article
Part of the following topical collections:
  1. Gene Expression


The protein phosphatase 2Cs (PP2Cs) have been demonstrated to act as negative modulators of protein kinase and to participate in stress signal transduction, as well as plant growth and productivity processes. Populus euphratica is so extraordinarily adaptable to abiotic stresses that it is regarded as a potential model plant for exploring resistance mechanisms of woody plants. To gain insight into the functional characteristics of PP2C genes in P. euphratica, 117 non-redundant PeuPP2C-encoding genes were identified from the whole genome. These members were classified into 13 groups (A–M), each of which was relatively conserved in gene structure and protein domain. A total of 39 paralogous pairs were found to be generated by whole genome duplication events, and Ka/Ks analysis indicated that these paralogous pairs had evolved mainly from purifying selection. The cis-acting elements and expression patterns showed that all the PeuPP2Cs were involved in response to single or multiple stresses including drought, salinity, heat, cold, and ABA. Taken together, our results summarized the genome-wide characterization of PeuPP2Cs and their expression profiling across different tissues and under multiple abiotic stresses in P. euphratica. These data provide a foundation to further investigate potential function of PeuPP2Cs in conferring tolerance to various stresses in P. euphratica.


Populus euphratica Protein phosphatase 2C (PP2C) Phylogenetic analysis Expression profiling Abiotic stress 



This work was supported by National Nonprofit Institute Research Grant of the Chinese Academy of Forestry (CAFYBB2018ZY001-9), (CAFYBB2017ZY008), and the National Natural Science Foundation of China (31570669) to JH.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interests.

Data archiving statement

All identified PP2C gene sequences of Populus euphratica were deposited into the NCBI database ( The accession numbers are listed in Table S2.

Supplementary material

11295_2018_1291_Fig7_ESM.png (57 kb)
Fig. S1

The expression levels of reporter genes for each condition tested. (PNG 56 kb)

11295_2018_1291_MOESM1_ESM.tiff (783 kb)
High Resolution Image (TIFF 782 kb)
11295_2018_1291_Fig8_ESM.png (587 kb)
Fig. S2

The number of each cis-acting element in the promoter region of each PeuPP2C gene. (PNG 587 kb)

11295_2018_1291_MOESM2_ESM.tiff (5.6 mb)
High Resolution Image (TIFF 5785 kb)
11295_2018_1291_MOESM3_ESM.xlsx (15 kb)
Table S1 Primers used in qRT-PCR analysis of the PeuPP2C genes. (XLSX 15 kb)
11295_2018_1291_MOESM4_ESM.xlsx (18 kb)
Table S2 The PP2C gene family identified in P. euphratica. (XLSX 18 kb)
11295_2018_1291_MOESM5_ESM.xlsx (102 kb)
Table S3 The complete coding sequences and the corresponding amino acid sequences of PP2C genes in P. euphratica. (XLSX 102 kb)
11295_2018_1291_MOESM6_ESM.xlsx (10 kb)
Table S4 The annotation of the cis-elements. (XLSX 10 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jianbo Li
    • 1
    • 2
  • Huixia Jia
    • 1
    • 3
  • Jin Zhang
    • 1
    • 3
  • Jingsuan Sun
    • 2
  • Yongan Zhang
    • 2
  • Mengzhu Lu
    • 1
    • 3
  • Xuebing Xin
    • 2
    Email author
  • Jianjun Hu
    • 1
    • 3
    Email author
  1. 1.State Key Laboratory of Tree Genetics and BreedingChinese Academy of ForestryBeijingChina
  2. 2.Experimental Center of Forestry in North ChinaChinese Academy of ForestryBeijingChina
  3. 3.Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of ForestryChinese Academy of ForestryBeijingChina

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