Genome-wide characterization of the basic leucine zipper transcription factors in Camellia sinensis
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The basic leucine zipper (bZIP) transcription factor family contains a large number of genes and has been found in many plant species. Previous studies have reported that bZIP family genes play important roles in the regulation of plant development and biotic/abiotic stress responses. However, a comprehensive investigation of this transcription factor family in tea plant (Camellia sinensis) has been lacking. In this study, a total of 61 bZIP genes were identified from the tea plant whole genome. The phylogenetic analysis classified these bZIP genes into 11 groups. The gene structures and composition patterns of the conserved motifs of bZIP genes were also characterized, which was consistent with the phylogenetic result. The gene expression analysis revealed that multiple bZIPs could be involved in response to drought, cold, or tea geometrid (Ectropis oblique) feeding. Our expression profile also indicated that many identified bZIPs were possibly involved in different developmental stages in tea plant. This study has provided an extensive overview of the tea plant bZIP transcription factor family and their possible roles in stress-responses.
KeywordsbZIP transcription factors Camellia sinensis Genome-wide Drought stress Cold stress Ectropis oblique feeding
This work was supported by the National Natural Science Foundation of China (31171608), the Special Innovative Province Construction in Anhui Province (15czs08032, 16czs08004), and the China Postdoctoral Science Foundation Grant (2016M601998) to Y. H. The authors thank Shi-Qi Zhao and Jun-Lan Wu (State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University) for fruitful discussions.
C. L. W. gave rise to and designed this project. Y. H. carried out the experiments and wrote the manuscript. A. L. W. and Y. X. H. performed the experiments. Y. H. and A. L. W. analyzed the data. F. D. L. contributed analysis tools.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Data archiving statement
In our analysis, 61 sequences of TEACsbZIPs have been found based on the assembled WGS dataset of Camellia sinensis. RNA-seq data of the eight tea plant tissues was produced to explore the global transcription profile of all identified TEACsbZIP genes.
All the nucleotide sequences of TEACsbZIPs will be submitted to GenBank (National Center for Biotechnology Information) and RNA-seq data of the eight tissues will be submitted to NCBI’s Transcriptome Shotgun Assembly Sequence Database (TSA). The accession numbers will be supplied once available.
- Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc Ser B Methodol 57:289–300Google Scholar
- ITC (2013) Annual bulletin of statistics 2013. ITC, London, pp 48–53Google Scholar
- Jin JP, Zhang H, Kong L, Gao G, Luo JC (2014) PlantTFDB 3.0: a portal for the functional and evolutionary study of plant transcription factors. Nucleic Acids Research, gkt1016. 42: D1182-D1187Google Scholar
- Kim HS, Delaney TP (2002) Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica. Plant J 32:151-163Google Scholar
- Matiolli CC, Tomaz JP, Duarte GT, Prado FM, Del Bem LE, Silveira AB, Gauer L, Correa LG, Drumond RD, Viana AJ, Di Mascio P, Meyer C, Vincentz M (2011) The Arabidopsis bZIP gene AtbZIP63 is a sensitive integrator of transient abscisic acid and glucose signals. Plant Physiol 157:692e705CrossRefGoogle Scholar
- Maung PP, He Q (2013) Determination of flavonoids and antioxidant activity in laphet laboratory process and tea (Camellia sinensis) products between China and Myanmar. International. J Eng Sci Technol 5:533–537Google Scholar
- Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30: 2725–2729Google Scholar
- Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K (2000) Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci 97:11632–11637CrossRefPubMedPubMedCentralGoogle Scholar