Tree Genetics & Genomes

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Genome-wide survey and analysis of the TIFY gene family and its potential role in anthocyanin synthesis in Chinese sand pear (Pyrus pyrifolia)

  • Yunjing Ma
  • Shashan Shu
  • Songling Bai
  • Ruiyan Tao
  • Minjie Qian
  • Yuanwen Teng
Original Article
  • 131 Downloads
Part of the following topical collections:
  1. Genome Biology

Abstract

The TIFY family is an important gene family which can be found only in plant and involved in different biologic processes. Jasmonic acid (JA) promotes anthocyanin accumulation in fruit. To explore the role of PpTIFY genes in anthocyanin biosynthesis of Chinese sand pear (Pyrus pyrifolia Nakai), we first cloned 21 PpTIFY genes from the pear genome and further identified 11 PpJAZ genes. The sequence similarity among the PpTIFY genes was relatively low, which indicated that PpTIFY genes in higher plants differentiated early during land plant evolution and have experienced considerable mutation. Transcripts of PpTIFY genes were detected in all organs and tissues of pear analyzed. The spatial and temporal expression patterns indicated that PpTIFY genes were associated with anthocyanin accumulation and JA signaling. The expression levels of PpTIFY genes were highest in leaves, whereas during fruit maturation, the expression level dramatically decreased. Furthermore, PpTIFY was induced after JA and light treatment in conjunction with anthocyanin accumulation in the peel of red fruit of Chinese sand pear. Genome-wide identification and characterization of pear PpTIFY genes will be helpful for further functional analysis of this gene family and cultivar improvement in pears.

Keywords

Chinese sand pear Anthocyanin PpTIFY Bioinformatics analysis Gene expression 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31471852) and the Earmarked Fund for China Agriculture Research System (CARS-28).

Compliance with ethical standards

Conflict of interest

The authors declare that they no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Supplementary material

11295_2018_1240_MOESM1_ESM.docx (6.6 mb)
ESM 1 (DOCX 6776 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yunjing Ma
    • 1
    • 2
    • 3
  • Shashan Shu
    • 1
    • 2
    • 3
  • Songling Bai
    • 1
    • 2
    • 3
  • Ruiyan Tao
    • 1
    • 2
    • 3
  • Minjie Qian
    • 1
    • 2
    • 3
  • Yuanwen Teng
    • 1
    • 2
    • 3
  1. 1.Department of HorticultureZhejiang UniversityZhejiangPeople’s Republic of China
  2. 2.The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture of ChinaZhejiangPeople’s Republic of China
  3. 3.Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural PlantsZhejiangPeople’s Republic of China

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