Genome-wide survey and analysis of the TIFY gene family and its potential role in anthocyanin synthesis in Chinese sand pear (Pyrus pyrifolia)
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The TIFY family is an important gene family which can be found only in plant and involved in different biologic processes. Jasmonic acid (JA) promotes anthocyanin accumulation in fruit. To explore the role of PpTIFY genes in anthocyanin biosynthesis of Chinese sand pear (Pyrus pyrifolia Nakai), we first cloned 21 PpTIFY genes from the pear genome and further identified 11 PpJAZ genes. The sequence similarity among the PpTIFY genes was relatively low, which indicated that PpTIFY genes in higher plants differentiated early during land plant evolution and have experienced considerable mutation. Transcripts of PpTIFY genes were detected in all organs and tissues of pear analyzed. The spatial and temporal expression patterns indicated that PpTIFY genes were associated with anthocyanin accumulation and JA signaling. The expression levels of PpTIFY genes were highest in leaves, whereas during fruit maturation, the expression level dramatically decreased. Furthermore, PpTIFY was induced after JA and light treatment in conjunction with anthocyanin accumulation in the peel of red fruit of Chinese sand pear. Genome-wide identification and characterization of pear PpTIFY genes will be helpful for further functional analysis of this gene family and cultivar improvement in pears.
KeywordsChinese sand pear Anthocyanin PpTIFY Bioinformatics analysis Gene expression
This work was supported by the National Natural Science Foundation of China (Grant No. 31471852) and the Earmarked Fund for China Agriculture Research System (CARS-28).
Compliance with ethical standards
Conflict of interest
The authors declare that they no conflict of interest.
This article does not contain any studies with animals performed by any of the authors.
- Bai S, Sun Y, Qian M, Yang F, Ni J, Tao R, Li L, Shu Q, Zhang D, Teng Y (2017) Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation. Sci Rep 7:63. https://doi.org/10.1038/s41598-017-00069-z CrossRefPubMedPubMedCentralGoogle Scholar
- Cerrudo I, Keller MM, Cargnel MD, Demkura PV, de Wit M, Patitucci MS, Pierik R, Pieterse CMJ, Ballare CL (2012) Low red/far-red ratios reduce Arabidopsis resistance to Botrytis cinerea and jasmonate responses via a COI1-JAZ10-dependent, salicylic acid-independent mechanism. Plant Physiol 158:2042–2052. https://doi.org/10.1104/pp.112.193359 CrossRefPubMedPubMedCentralGoogle Scholar
- Chini A, Fonseca S, Fernández G, Adie B, Chico JM, Lorenzo O, García-Casado G, López-Vidriero I, Lozano FM, Ponce MR, Micol JL, Solano R (2007) The JAZ family of repressors is the missing link in jasmonate signalling. Nature 448:666–U664. https://doi.org/10.1038/nature06006 CrossRefPubMedGoogle Scholar
- Devoto A, Ellis C, Magusin A, Chang HS, Chilcott C, Zhu T, Turner JG (2005) Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions. Plant Mol Biol 58:497–513. https://doi.org/10.1007/s11103-005-7306-5 CrossRefPubMedGoogle Scholar
- Finn RD, Coggill P, Eberhardt RY, Eddy SR, Mistry J, Mitchell AL, Potter SC, Punta M, Qureshi M, Sangrador-Vegas A, Salazar GA, Tate J, Bateman A (2016) The Pfam protein families database: towards a more sustainable future. Nucleic Acids Res 44:D279–D285. https://doi.org/10.1093/nar/gkv1344 CrossRefPubMedGoogle Scholar
- Gutierrez L, Mongelard G, Floková K, Păcurar DI, Novák O, Staswick P, Kowalczyk M, Păcurar M, Demailly H, Geiss G, Bellini C (2012) Auxin controls Arabidopsis adventitious root initiation by regulating jasmonic acid homeostasis. Plant Cell 24:2515–2527. https://doi.org/10.1105/tpc.112.099119 CrossRefPubMedPubMedCentralGoogle Scholar
- Jiang C, Gao X, Liao L, Harberd NP, Fu X (2007) Phosphate starvation root architecture and anthocyanin accumulation responses are modulated by the gibberellin-DELLA signaling pathway in Arabidopsis. Plant Physiol 145:1460–1470. https://doi.org/10.1104/pp.107.103788 CrossRefPubMedPubMedCentralGoogle Scholar
- Loreti E, Povero G, Novi G, Solfanelli C, Alpi A, Perata P (2008) Gibberellins, jasmonate and abscisic acid modulate the sucrose-induced expression of anthocyanin biosynthetic genes in Arabidopsis. New Phytol 179:1004–1016. https://doi.org/10.1111/j.1469-8137.2008.02511.x CrossRefPubMedGoogle Scholar
- Nishii A, Takemura M, Fujita H, Shikata M, Yokota A, Kohchi T (2000) Characterization of a novel gene encoding a putative single zinc-finger protein, ZIM, expressed during the reproductive phase in Arabidopsis thaliana. Biosci Biotechnol Biochem 64:1402–1409. https://doi.org/10.1271/bbb.64.1402 CrossRefPubMedGoogle Scholar
- Niu Q, Li J, Cai D, Qian M, Jia H, Bai S, Hussain S, Liu G, Teng Y, Zheng X (2016) Dormancy-associated MADS-box genes and microRNAs jointly control dormancy transition in pear (Pyrus pyrifolia white pear group) flower bud. J Exp Bot 67:239–257. https://doi.org/10.1093/jxb/erv454 CrossRefPubMedGoogle Scholar
- Qi TC, Song S, Ren Q, Wu D, Huang H, Chen Y, Fan M, Peng W, Ren C, Xie D (2011) The jasmonate-ZIM-domain proteins interact with the WD-repeat/bHLH/MYB complexes to regulate jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana. Plant Cell 23:1795–1814. https://doi.org/10.1105/tpc.111.083261 CrossRefPubMedPubMedCentralGoogle Scholar
- Qian MJ, Yu B, Li X, Sun YW, Zhang D, Teng YW (2014) Isolation and expression analysis of anthocyanin biosynthesis genes from the red Chinese sand pear, Pyrus pyrifolia Nakai cv. Mantianhong, in response to methyl jasmonate treatment and UV-B/Vis conditions. Plant Mol Biol Report 32:428–437. https://doi.org/10.1007/s11105-013-0652-6 CrossRefGoogle Scholar
- Ren CM, Han C, Peng W, Huang Y, Peng Z, Xiong X, Zhu Q, Gao B, Xie D (2009) A leaky mutation in DWARF4 reveals an antagonistic role of Brassinosteroid in the inhibition of root growth by jasmonate in Arabidopsis. Plant Physiol 151:1412–1420. https://doi.org/10.1104/pp.109.140202 CrossRefPubMedPubMedCentralGoogle Scholar
- Shan LL, Li X, Wang P, Cai C, Zhang B, Sun CD, Zhang WS, Xu CJ, Ferguson I, Chen KS (2008) Characterization of cDNAs associated with lignification and their expression profiles in loquat fruit with different lignin accumulation. Planta 227:1243–1254. https://doi.org/10.1007/s00425-008-0696-2 CrossRefPubMedGoogle Scholar
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739. https://doi.org/10.1093/molbev/msr121 CrossRefPubMedPubMedCentralGoogle Scholar
- Yang Y, Yao G, Yue W, Zhang S, Wu J (2015) Transcriptome profiling reveals differential gene expression in proanthocyanidin biosynthesis associated with red/green skin color mutant of pear (Pyrus communis L.) Front Plant Sci 6:795. https://doi.org/10.3389/fpls.2015.00795 PubMedPubMedCentralGoogle Scholar
- van Zanten M, Ritsema T, Polko JK, Leon-Reyes A, Voesenek LACJ, Millenaar FF, Pieterse CMJ, Peeters AJM (2012) Modulation of ethylene- and heat-controlled hyponastic leaf movement in Arabidopsis thaliana by the plant defence hormones jasmonate and salicylate. Planta 235:677–685. https://doi.org/10.1007/s00425-011-1528-3 CrossRefPubMedGoogle Scholar
- Zhang D, Yu B, Bai JH, Qian MJ, Shu Q, Su J, Teng YW (2012a) Effects of high temperatures on UV-B/visible irradiation induced postharvest anthocyanin accumulation in 'Yunhongli No. 1' (Pyrus pyrifolia Nakai) pears Sci Hortic 134:53–59. https://doi.org/10.1016/j.scienta.2011.10.025