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ALDH2 genes are negatively correlated with natural deastringency in Chinese PCNA persimmon (Diospyros kaki Thunb.)

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Abstract

Chinese pollination-constant and non-astringent persimmon (C-PCNA) has important application values in the genetic improvement of PCNA for its trait of natural deastringency controlled by a single dominant gene. However, the key genes and the regulatory networks are still not fully understood. The process of C-PCNA natural deastringency may be associated with the acetaldehyde-mediated coagulation of soluble tannins, but the functions of ALDH2 genes related to the metabolism of acetaldehyde are not clear. In this work, three types of persimmon cultivars, ‘Eshi 1’ and ‘Luotian Tianshi’ (C-PCNA type), ‘Youhou’ (J-PCNA type), and ‘Mopanshi’ (non-PCNA type), were sampled. Two members of ALDH2 family genes, DkALDH2a and DkALDH2b, were isolated from ‘Eshi 1’ persimmon fruit. Gene expression patterns indicated that they may be involved in “coagulation effect”, which leads to natural deastringency in C-PCNA persimmon fruit. Transient expression in ‘Eshi 1’ leaves further demonstrated that their expression can reduce the consumption of soluble tannins and inhibit the astringency removal process. Therefore, DkALDH2a and DkALDH2b are negatively correlated with natural deastringency in C-PCNA persimmon.

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Acknowledgments

This research was supported by the Natural Science Foundation of China (31471861) and Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains (2015TD01) as well as Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization (2017BX07).

Data archiving statement

The cDNA sequences of DkALDH2a and DkALDH2b genes have been submitted to GenBank, and the accession numbers are MG189594 and MG189595, respectively.

Author information

Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan, Hubei, 430070, China

    Jun-Chi Xu, Jin-Ge Ding, Rong-Li Mo, Li-Qing Xu, Qing-Lin Zhang & Zheng-Rong Luo

  2. Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, Hubei, 438000, China

    Jun-Chi Xu, Jian-Ping Gan & Zheng-Rong Luo

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  1. Jun-Chi Xu
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  2. Jin-Ge Ding
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  3. Jian-Ping Gan
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  4. Rong-Li Mo
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  5. Li-Qing Xu
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Correspondence to Zheng-Rong Luo.

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Additional information

Communicated by A. M. Dandekar

Electronic supplementary material

Fig. S1

Photograph of three types of persimmon fruits at different developmental stages. Scale bar = 5 cm (GIF 170 kb)

High resolution (TIFF 30422 kb)

Fig. S2

Protein sequences of ALDH2a alignment between ‘Luotian Tianshi’ and ‘Eshi 1’ (GIF 17 kb)

Fig. S3

Protein sequences of ALDH2b alignment between ‘Luotian Tianshi’ and ‘Eshi 1’ (GIF 18 kb)

Table S1

Sequences of the primers used for qRT-PCR analysis (DOCX 13 kb)

Table S2

Sequences of the primers used for DkALDH2a and DkALDH2b gene amplification by RACE (DOCX 13 kb)

Table S3

Sequences of the primers used for DkALDH2a and DkALDH2b gene ORFs amplification (DOCX 13 kb)

Table S4

Sequences of the primers used for DkALDH2a and DkALDH2b gene silencing fragments amplification (DOCX 13 kb)

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Xu, JC., Ding, JG., Gan, JP. et al. ALDH2 genes are negatively correlated with natural deastringency in Chinese PCNA persimmon (Diospyros kaki Thunb.). Tree Genetics & Genomes 13, 122 (2017). https://doi.org/10.1007/s11295-017-1207-z

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  • DOI: https://doi.org/10.1007/s11295-017-1207-z

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