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Field-based artificial crossings indicate partial compatibility of reciprocal crosses between Pinus sylvestris and Pinus mugo and unexpected chloroplast DNA inheritance

  • Andrej Kormutak
  • Martin Galgoci
  • Peter Manka
  • Martina Koubova
  • Martin Jopcik
  • Denisa Sukenikova
  • Peter Bolecek
  • Dusan Gőmőry
Original Article
Part of the following topical collections:
  1. Hybridization

Abstract

Crossability relationships between Scots pine (Pinus sylvestris L.) and mountain dwarf pine (Pinus mugo Turra) was studied, using artificial pollination approach. Partial compatibility of the reciprocal crossings of these species was proved experimentally, validating the idea of a spontaneous formation of their hybrid swarms under natural conditions. The hybrids were validated using organellar DNA markers and nuclear DNA microsatellites. Based on the percentage of filled seeds, the interspecific crossings were less efficient than the intraspecific cross-pollinations of P. sylvestris and P. mugo individuals. Both species were found to intercross readily with individuals of their putative hybrid swarm, P. mugo exhibiting a higher hybridological affinity towards putatively hybrid individuals than P. sylvestris. Validation of the hybrids confirmed the paternal inheritance of chloroplast DNA (cpDNA) in the combination P. sylvestris × P. mugo only. Surprisingly, in the reciprocal crossing P. mugo × P. sylvestris, maternal inheritance of cpDNA was revealed. Obtained results offer a new insight into the direction and intensity of gene flow within the hybrid swarms of Scots pine and mountain dwarf pine.

Keywords

Pinus sylvestris L. Pinus mugo Turra Hybridization DNA 

Notes

Acknowledgements

The authors are grateful to the anonymous reviewers for their valuable and inspiring comments which helped to improve the quality of the manuscript. The contribution of the associated editor in finalizing the manuscript is appreciated either. The study was funded by the COST European Cooperation in Science and Technology, Brussels, project COST Action FP 1403, VEGA Grant Agency, project no. 2/0063/17, and the Operational Programme Research and Development project ITMS 26220220192.

Data archiving statement

No data were provided for public database.

Supplementary material

11295_2017_1152_MOESM1_ESM.docx (20 kb)
Table S1 (DOCX 20 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Andrej Kormutak
    • 1
    • 2
  • Martin Galgoci
    • 1
  • Peter Manka
    • 3
  • Martina Koubova
    • 1
  • Martin Jopcik
    • 1
  • Denisa Sukenikova
    • 2
  • Peter Bolecek
    • 2
  • Dusan Gőmőry
    • 4
  1. 1.Plant Science and Biodiversity Center, Slovak Academy of Sciences, Institute of Plant Genetics and BiotechnologyNitraSlovak Republic
  2. 2.Faculty of Natural SciencesConstantine Philosopher UniversityNitraSlovak Republic
  3. 3.Department of State Administration of Nature ProtectionMinistry of EnvironmentBratislavaSlovak Republic
  4. 4.Faculty of ForestryTechnical University in ZvolenZvolenSlovak Republic

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