Comparison and confirmation of SNP-bud burst associations in European beech populations in Germany

Original Article
Part of the following topical collections:
  1. Complex Traits

Abstract

European beech (Fagus sylvatica L.) is one of the most important forest tree species in Central Europe. Climate change scenarios predict an increase in annual mean temperatures that may cause earlier bud burst in spring, potentially leading to an increased late frost-risk. Despite the ecologic and economic importance of beech, knowledge about the molecular basis of bud burst is still scarce in this species. Here, an association analysis was used to detect SNPs that are significantly associated with beech bud burst. A translocation experiment was established with progenies of six different beech populations from three widely separated regions in Northeast, Central, and Southwest Germany. In total, 600 individuals of the translocation experiment were genotyped using a set of 46 SNPs located in bud burst candidate genes. The association analysis revealed seven SNPs significantly associated with bud burst, each SNP explaining only a few percent of the observed phenotypic variation. Since the same SNP set was used in a previous association analysis with European beech, we were able to compare and confirm significant associations between SNPs and bud burst in distinct beech populations growing in different environments.

Keywords

European beech Bud burst Association Climate change 

Notes

Acknowledgements

We thank the managers of the three Exploratories, Kirsten Reichel-Jung, Swen Renner, Katrin Hartwich, Sonja Gockel, Kerstin Wiesner, and Martin Gorke for their work in maintaining the plot and project infrastructure, Christiane Fischer and Simone Pfeiffer for giving support through the central office, Michael Owonibi for managing the central data base, and Markus Fischer, Eduard Linsenmair, Dominik Hessenmöller, Jens Nieschulze, Daniel Prati, Ingo Schöning, François Buscot, Ernst-Detlef Schulze, Wolfgang W. Weisser, and the late Elisabeth Kalko for their role in setting up the Biodiversity Exploratories project. The work has been funded by the DFG Priority Program 1374 “Infrastructure-Biodiversity-Exploratories” (Fi 569/12-2). Field work permits were issued by the responsible state environmental offices of Baden-Württemberg, Thüringen, and Brandenburg (according to § 72 BbgNatSchG).

We thank everyone who helped us with field and/or lab work, in particular Christine Radler, Alexandra Dolynska, Gerold Dinkel, Marco Winkler, Melanie Schmitt, Laura Cuervo, Natalie Breidenbach, Kristina Schröter, and Rodica Pena.

We greatly appreciate the comments by three anonymous reviewers, who helped to improve the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Data archiving statement

Genotypic (SNP/SSR) and phenotypic data were submitted to the TreeGenes Database (http://dendrome.ucdavis.edu/treegenes). The accession number is TGDR062.

Supplementary material

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© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Forest Genetics and Forest Tree BreedingBüsgen Institute, Faculty for Forest Sciences and Forest Ecology, Georg-August University GöttingenGöttingenGermany
  2. 2.University of KasselKasselGermany

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