Revisiting the provenance delineation of a widespread shrub, Frangula alnus—the role of spatial, temporal and environmental patterns
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Including population genetic aspects into the selection of planting material within the framework of conservation and restoration measures is of vital importance for the long-term persistence of populations. This is especially true facing climate change since genetic diversity and the spread of potentially beneficial alleles are important for the adaptability of populations. Therefore, knowledge about genetic variability within and between populations is a critical aspect when determining provenance regions. In our study, we investigated the population genetic structure of a widespread, insect-pollinated and mainly bird-dispersed shrub species, Frangula alnus, on the basis of seven microsatellites and two chloroplast DNA markers throughout Germany. The aim was to determine the spatial, temporal and ecological processes genetically structuring populations to critically revise existing provenance regions. Therefore, we conducted analyses on different spatial scales (country-wide, regional and local) using the two different marker sets in addition to environmental variables. We detected distinct patterns on all spatial scales which indicated influences of historic recolonization processes, regional differences of seed dispersal across the landscape, as well as small-scale spatial genetic structures attributable to local dispersal processes. No relation of underlying environmental gradients such as temperature or precipitation and genetic patterns was found. We conclude that different aspects of historic and more recent gene flow shape population genetic structures and that a thorough analysis on a variety of spatial, temporal and environmental scales is necessary to appropriately select planting material for conservation and restoration measures. Correspondingly, management advice regarding provenance delineations will be provided.
KeywordsSeed zone Microsatellite cpDNA Genetic diversity Differentiation Management
We are very grateful to all the forest rangers, colleagues and friends who supported this study by collecting leaf material for the genetic analysis throughout Germany. Many thanks go to Christina Mengel and Sascha Liepelt for their support and helpful advices regarding the laboratory work. Moreover, we would like to thank two anonymous reviewers for their helpful comments. This project was funded by the program “Forschung für die Praxis” by the Hesse State Ministry of Higher Education, Research and the Arts.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
Data archiving statement
Genotyping data of all collected samples for the seven microsatellites as well as cpDNA data of the corresponding number of samples are available from the Dryad Digital Repository: http://dx.doi.org/10.5061/dryad.215sn.
- Barsch F, Heym A, Nehring S (2012) Leitfaden zur Verwendung gebietseigener Gehölze. https://www.bfn.de/fileadmin/BfN/recht/Dokumente/leitfaden_gehoelze_.pdf. Accessed 25 July 2015
- Bucci G, Gonzalez-Martinez SC, Le Provost G, Plomion C, Ribeiro MM, Sebastiani F, Alia R, Vendramin GG (2007) Range-wide phylogeography and gene zones in Pinus pinaster Ait. revealed by chloroplast microsatellite markers. Mol Ecol 16(10):2137–2153. doi: 10.1111/j.1365-294X.2007.03275.x CrossRefPubMedGoogle Scholar
- Cornille A, Giraud T, Bellard C, Tellier A, Le Cam B, Smulders MJM, Kleinschmit J, Roldan-Ruiz I, Gladieux P (2013) Postglacial recolonization history of the European crabapple (Malus sylvestris Mill.), a wild contributor to the domesticated apple. Mol Ecol 22(8):2249–2263. doi: 10.1111/mec.12231 CrossRefPubMedGoogle Scholar
- De Kort H, Mergeay J, Vander Mijnsbrugge K, Decocq G, Maccherini S, Kehlet Bruun HH, Honnay O, Vandepitte K, Bugmann H (2014) An evaluation of seed zone delineation using phenotypic and population genomic data on black alder Alnus glutinosa. J Appl Ecol 51(5):1218–1227. doi: 10.1111/1365-2664.12305 CrossRefGoogle Scholar
- De Kort H, Vandepitte K, Mergeay J, Mijnsbrugge KV, Honnay O (2015) The population genomic signature of environmental selection in the widespread insect-pollinated tree species Frangula alnus at different geographical scales. Heredity 115(5):415–425. doi: 10.1038/hdy.2015.41 CrossRefPubMedPubMedCentralGoogle Scholar
- GeoBasis-DE/BKG (2015) Digitales geländemodell gitterweite 200m. Bundesamt für Kartographie und Geodäsie. Dataset: http://www.geodatenzentrum.de/geodaten/gdz_rahmen.gdz_div?gdz_spr=deu&gdz_akt_zeile=5&gdz_anz_zeile=1&gdz_unt_zeile=3&gdz_user_id=0
- Hampe A, Petit RJ (2005) Conserving biodiversity under climate change: the rear edge matters. Ecol Lett 8(5):461–467. doi: 10.1111/j.1461-0248.2005.00739.x
- Heuertz M, Fineschi S, Anzidei M, Pastorelli R, Salvini D, Paule L, Frascaria-Lacoste N, Hardy OJ, Vekemans X, Vendramin GG (2004) Chloroplast DNA variation and postglacial recolonization of common ash (Fraxinus excelsior L.) in Europe. Mol Ecol 13(11):3437–3452. doi: 10.1111/j.1365-294X.2004.02333.x CrossRefPubMedGoogle Scholar
- Kremer A, Ronce O, Robledo-Arnuncio JJ, Guillaume F, Bohrer G, Nathan R, Bridle JR, Gomulkiewicz R, Klein EK, Ritland K, Kuparinen A, Gerber S, Schueler S (2012) Long-distance gene flow and adaptation of forest trees to rapid climate change. Ecol Lett 15(4):378–392. doi: 10.1111/j.1461-0248.2012.01746.x CrossRefPubMedPubMedCentralGoogle Scholar
- Lavabre JE, Gilarranz LJ, Fortuna MA, Bascompte J (2016) How does the functional diversity of frugivorous birds shape the spatial pattern of seed dispersal?: a case study in a relict plant species. Phil Trans R Soc B 371(1694):20150280. doi: 10.1098/rstb.2015.0280 CrossRefPubMedPubMedCentralGoogle Scholar
- Oksanen JF, Blanchet G, Kindt R, Legendre P, Minchin PR, O’Hara RB, Simpson GL, Solymos P, Stevens MHH, Wagner H (2015) vegan: Community Ecology Package. R package version 2.3–1. http://CRAN.R-project.org/package=vegan. Accessed 12 November 2015
- Petit R, Aguinagalde I, de Beaulieu JL, Bittkau C, Brewer S, Cheddadi R, Ennos R, Fineschi S, Grivet D, Lascoux M, Mohanty A, Müller-Starck G, Demesure-Musch B, Palmé A, Martín JP, Rendell S, Vendramin GG (2003) Glacial refugia: hotspots but not melting pots of genetic diversity. Science 300(5625):1563–1565. doi: 10.1126/science.1083264 CrossRefPubMedGoogle Scholar
- R Development Core Team (2014) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. URL http://www.R-project.org
- Reiker J, Schulz B, Wissemann V, Gemeinholzer B (2015) Does origin always matter? Evaluating the influence of nonlocal seed provenances for ecological restoration purposes in a widespread and outcrossing plant species. Ecol Evol 5(23):5642–5651. doi: 10.1002/ece3.1817 CrossRefPubMedPubMedCentralGoogle Scholar
- Zecchin B, Caudullo G, Rigo DD (2016) Frangula alnus in Europe: distribution, habitat, usage and threats. In: San-Miguel-Ayanz J, de Rigo D, Caudullo G, Houston Durrant T, Mauri A (eds) European Atlas of Forest Tree Species. Publication Ofice EU, Luxembourg, p e019ee2+ https://w3id.org/mtv/FISE-Comm/v01/e019ee2. Accessed 25.01.2017Google Scholar