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Tree Genetics & Genomes

, 12:108 | Cite as

Polygenic inheritance of resistance to Cacopsylla pyri in a Pyrus communis × P. ussuriensis progeny is explained by three QTLs involving an epistatic interaction

  • Perchepied L.
  • Guérif P.
  • Ravon E.
  • Denancé C.
  • Laurens F.
  • Robert P.
  • Bouvier L.
  • Lespinasse Y.
  • Durel C.E.
Original Article
Part of the following topical collections:
  1. Complex Traits

Abstract

Pear psylla (Cacopsylla pyri) causes severe damage on European pear cultivars, resulting in high yield losses. Its control has become difficult since it developed resistance to a wide range of pesticides, while the number of authorized molecules for pest control has decreased. Identifying pear psylla resistance factors should help breeding new resistant pear cultivars. We analyzed the quantitative resistance to psylla inherited from the genotype NY 10355 derived from Pyrus ussuriensis. Quantitative trait locus (QTL) analysis was carried out after counting the number of nymphs and estimating the nymphal development rate using a free-choice test performed on a large segregating progeny. We mapped two new loci for pear psylla resistance on linkage groups LG01 and LG04 of NY 10355 and confirmed the QTL previously detected on LG17. A strong epistatic interaction between the two QTLs detected on LG01 and LG17 appeared to be a major factor controlling the psylla infestation in the genotype NY 10355.

Keywords

Epistasis QTLs Pear Psylla Interspecific 

Notes

Acknowledgments

We greatly thank Rémi Guisnel, André Belouin, Bernard Petit, François Lebreton, Maryline Bruneau, Roland Robic, and Yves Rabineau (UMR1345 IRHS, Angers, France) for their precious help in the scoring of psylla nymphs. We thank Pierre-Olivier Fongueuse for taking care of the psylla. We thank Hélène Muranty for her help in the statistical analyses. We thank Charles Poncet and Laurence Hibrand-Saint Oyant for their genotyping technical assistance delivered by the platforms GENTYANE of Clermont-Ferrand-Theix (http://gentyane.clermont.inra.fr/) and ANAN of the SFR QUASAV (Structure Fédérative de Recherche 4207), respectively. We also thank the UE Horti experimental unit and the team in charge of the greenhouse management (INEM platform) for taking care of the plant material.

Compliance with ethical standards

Data archiving statement

Phenotypic, markers, and QTL data will be made publicly available through the Genome Database for Rosaceae (www.rosaceae.org).

Supplementary material

11295_2016_1072_MOESM1_ESM.pdf (36 kb)
ESM 1 (PDF 35 kb)
11295_2016_1072_MOESM2_ESM.pdf (10 kb)
ESM 2 (PDF 9 kb)
11295_2016_1072_MOESM3_ESM.pdf (11 kb)
ESM 3 (PDF 10 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Perchepied L.
    • 1
  • Guérif P.
    • 1
  • Ravon E.
    • 1
  • Denancé C.
    • 1
  • Laurens F.
    • 1
  • Robert P.
    • 1
  • Bouvier L.
    • 1
  • Lespinasse Y.
    • 1
  • Durel C.E.
    • 1
  1. 1.IRHS, INRA, AGROCAMPUS-OuestUniversité d’Angers, SFR 4207 QUASAVBeaucouzé cedexFrance

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