Tree Genetics & Genomes

, 12:94

Transcriptome analysis of the male-to-hermaphrodite sex reversal induced by low temperature in papaya

Original Article
Part of the following topical collections:
  1. Gene Expression


Papaya (Carica papaya L.) is a trioecious plant species, producing three sex forms, male, female, and hermaphrodite. Although the major sex types are genetically determined, the phenotypic sex expression of papaya is influenced by environmental factors. We investigated differential gene expression analysis between the non-functional rudimentary pistils from normal male flowers and developed and functional pistils from the male-to-hermaphrodite sex reversal flowers induced by low temperature aiming to understanding the gene regulatory network that determinates the phenotypic sex expression in papaya. Our differential gene expression analysis revealed 1756 differentially expressed genes between normal male and male-to-hermaphrodite sex reversal flowers. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analysis showed transcription factors, flower development, histone H3-K9 methylation, and plant hormone signal transduction were among the most significantly enriched GO terms and KEGG pathways. Small RNA analysis was also performed on the pistils from normal male and the male-to-hermaphrodite sex reversal flowers. Our result showed the 24 nt small RNAs were the most abundant in the pistils from both normal and sex reversal flowers, followed by 21 nt small RNAs. We detected expression of 40 plant-conserved miRNAs and 14 papaya-specific miRNAs in the pistils from one or both normal and sex reversal flowers. Sixteen miRNAs exhibited high-expression level and ten of them showed differential expression between the normal male and the male-to-hermaphrodite sex reversal flowers. Our results suggested the male-to-hermaphrodite sex reversal was likely caused by silencing the gynoecium suppression function on the sex determination pathway through epigenetic modification.


Carica papaya Sex determination Sex reversal Differential gene expression Small RNA 

Supplementary material

11295_2016_1055_MOESM1_ESM.xlsx (14 kb)
ESM 1(XLSX 13 kb)
11295_2016_1055_MOESM2_ESM.xlsx (180 kb)
ESM 2(XLSX 179 kb)
11295_2016_1055_MOESM3_ESM.docx (48 kb)
ESM 3(DOCX 47 kb)
11295_2016_1055_MOESM4_ESM.xlsx (15 kb)
ESM 4(XLSX 15 kb)
11295_2016_1055_MOESM5_ESM.xlsx (19 kb)
ESM 5(XLSX 18 kb)
11295_2016_1055_MOESM6_ESM.xlsx (27 kb)
ESM 6(XLSX 26 kb)
11295_2016_1055_MOESM7_ESM.docx (18 kb)
ESM 7(DOCX 18 kb)
11295_2016_1055_MOESM8_ESM.xlsx (10 kb)
ESM 8(XLSX 10 kb)
11295_2016_1055_MOESM9_ESM.xlsx (11 kb)
ESM 9(XLSX 10 kb)
11295_2016_1055_MOESM10_ESM.xlsx (18 kb)
ESM 10(XLSX 18 kb)
11295_2016_1055_MOESM11_ESM.xlsx (14 kb)
ESM 11(XLSX 14 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Hai Lin
    • 1
  • Zhenyang Liao
    • 1
  • Lingmao Zhang
    • 1
  • Qingyi Yu
    • 1
    • 2
    • 3
  1. 1.Center for Genomics and Biotechnology, Fujian Provincial Key laboratory of Haixia applied plant systems biology, Haixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhouChina
  2. 2.Texas A&M AgriLife Research Center at DallasTexas A&M University SystemDallasUSA
  3. 3.Department of Plant Pathology & MicrobiologyTexas A&M UniversityCollege StationUSA

Personalised recommendations