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A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4

Abstract

MYB-bHLH-WD40 (MBW) ternary complexes composed of MYB domain proteins, basic helix-loop-helix (bHLH) transcription factors and WD40-repeat proteins are involved in the transcriptional regulation of anthocyanin and proanthocyanidin (PA) biosynthesis pathways in distinct plant species. PAs are the compounds responsible for the strong astringent taste of persimmon fruit. DkMYB2 and DkMYB4 (MYB type) and DkMYC1 (bHLH type) genes from persimmon have been postulated to regulate the biosynthesis of PAs in fruit. We have identified a WD40-repeat gene from persimmon (DkWDR1) coding for a protein highly similar to TTG1 from Arabidopsis thaliana and other WD40-repeat components of distinct MBW complexes. DkWDR1 expression was detected in different tissues and was essentially constant during fruit development. MYB-like proteins DkMYB2 and DkMYB4 interacted with both DkWDR1 and DkMYC1 in the yeast two-hybrid system. The sequence of DkWDR1 and the analysis of yeast two-hybrid interactions with known regulators of PA biosynthesis support the occurrence of MYB-bHLH-WD40 (MBW) complexes in persimmon, to regulate PA accumulation in fruit.

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Acknowledgments

This work was funded by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-FEDER (grant number RF2013-00043-C02-02) and the Ministry of Science and Innovation of Spain (grant number AGL2010-20595). FG-M and ALL were funded by a fellowship co-financed by the European Social Fund and the Instituto Valenciano de Investigaciones Agrarias (IVIA).

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Corresponding author

Correspondence to Gabino Ríos.

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Conflict of interest

The authors declare that they have no competing interests. In addition the research is not involving human participants or animals.

Data archiving statement

NCBI GenBank accession numbers are provided in Online Resource Table S3.

Additional information

Communicated by A.M. Dandekar

Electronic supplementary material

Below is the link to the electronic supplementary material.

Online Resource Table S1

Primers used in this study. Primers obtained or modified from Akagi et al. (2009b) are labelled with (*). (PDF 46 kb)

Online Resource Table S2

Stability index of gene expression. (PDF 27 kb)

Online Resource Table S3

Allele composition of DkWDR1 in ‘Hachiya’ cultivar. (PDF 32 kb)

Online Resource Fig. S1

Alignment of DkWDR1 alleles and deduced proteins. (PDF 56 kb)

Online Resource Fig. S2

Polymorphisms of DkWDR1 in the non-astringent variety ‘Hana Fuyu’ by sequencing of a pooled PCR product. (PDF 46 kb)

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Naval, M.d.M., Gil-Muñoz, F., Lloret, A. et al. A WD40-repeat protein from persimmon interacts with the regulators of proanthocyanidin biosynthesis DkMYB2 and DkMYB4. Tree Genetics & Genomes 12, 13 (2016). https://doi.org/10.1007/s11295-016-0969-z

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  • DOI: https://doi.org/10.1007/s11295-016-0969-z

Keywords

  • Diospyros kaki
  • Fruit astringency
  • Tannin
  • MBW complex
  • WD40 repeat
  • Protein interaction