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Identification and characterization of the EIN3/EIL gene family in Hevea brasiliensis

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Abstract

Ethylene-insensitive3/ethylene-insensitive3-like (EIN3/EIL) transcription factor is a key transcription factor involved in the ethylene signal transduction pathway in plants. Although the genome-wide analysis of this family has been carried out in certain plant species, little is known about EIN3/EIL genes in rubber tree (Hevea brasiliensis). In this study, we identified four EIN3/EIL genes (designated as HbEIN3-1 to HbEIN3-4) in the rubber tree genome annotation. Tissue-specific expression profiles showed that four HbEIN3s were expressed in at least one of the tested tissues. The four HbEIN3s responded to ethylene (ET) and jasmonic acid (JA) treatment, which suggested that HbEIN3s were involved in response to JA and ET signaling. HbEIN3s showed transactivation activity in yeast. HbEIN3-1 bound to the promoter of the rubber transferase gene, suggesting that HbEIN3-1 may be involved in the regulation of natural rubber biosynthesis. The N-terminal basic domain II was required for the HbEIN3-1 binding with the promoter of the rubber transferase gene.

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Abbreviations

3-AT:

3-Amino-1,2,4-triazole

DBD:

DNA-binding domain

EIN3/EIL:

Ethylene-insensitive3/ethylene-insensitive3-like

ET:

Ethylene

JA:

Jasmonate

NR:

Natural rubber

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Acknowledgments

This research was supported by National Natural Science Foundation of China (No. 31170634) and the Major Technology Project of Hainan (ZDZX2013023-1). Chinese Academy of Tropical Agricultural Sciences and Hainan University contributed equally to this work.

Data archiving statement

Nucleotide sequences were deposited with the GenBank. HbEIN3-1 (GenBank accession No. KR013139); HbEIN3-2 (GenBank accession No. KR013140); HbEIN3-3 (GenBank accession No. KR013141); HbEIN3-4 (GenBank accession No. KR013142).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Zi-Ping Yang, Hui-Liang Li, Dong Guo & Shi-Qing Peng

  2. College of Agriculture, Hainan University, Haikou, 570228, China

    Zi-Ping Yang & Shi-Qing Peng

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  1. Zi-Ping Yang
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  2. Hui-Liang Li
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  3. Dong Guo
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  4. Shi-Qing Peng
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Correspondence to Shi-Qing Peng.

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Communicated by W. Ratnam

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Yang, ZP., Li, HL., Guo, D. et al. Identification and characterization of the EIN3/EIL gene family in Hevea brasiliensis . Tree Genetics & Genomes 11, 51 (2015). https://doi.org/10.1007/s11295-015-0877-7

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  • DOI: https://doi.org/10.1007/s11295-015-0877-7

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