Abstract
Grapevine cultivars are planted in worldwide viticulture and are asexually propagated. Horticultural clones are asexually derived from a single individual, and clonal variation can only occur through mutations. Molecular markers are an important tool for the differentiation and identification of clones and mutations. For breeding purposes and clonal selection, knowledge upon the variability of a given clone is essential. The aim of this study was to assess amplified fragment length polymorphism (AFLP) markers for classifying mutations in 86 Riesling clones of Vitis vinifera and to enhance our understanding on the dynamic of grapevine clones analysed by AFLP fingerprints. AFLP markers detected 135 polymorphic bands of a total amount of 305 bands. AFLP markers detected two different types of mutations: single-event mutations, only detected once in one clone, displaying the variation of the grape genome and specific loci mutations where the mutation could be found frequently in the set of clones and therefore stand for the stability of grapevine genome. A general grouping of clones according to age, sub-clonal lineage or origin could not be determined by the set of AFLP markers employed.
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Acknowledgements
We thank Roswitha Mammerler and Nathalie Bellutti for their help in DNA extraction and genotyping and all the donors of Riesling clones who contributed to the clone collection used in the present study.
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Communicated by R. Velasco
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Anhalt, U.C.M., Crespo Martínez, S., Rühl, E. et al. Dynamic grapevine clones—an AFLP-marker study of the Vitis vinifera cultivar Riesling comprising 86 clones. Tree Genetics & Genomes 7, 739–746 (2011). https://doi.org/10.1007/s11295-011-0370-x
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DOI: https://doi.org/10.1007/s11295-011-0370-x